Marker-Assisted Introgression of Multiple Resistance Genes Confers Broad Spectrum Resistance against Bacterial Leaf Blight and Blast Diseases in PUTRA-1 Rice Variety
Bacterial leaf blight caused by <i>Xanthomonas oryzae</i> pv <i>oryzae</i> (<i>Xoo</i>) and blast caused by <i>Magnaporthe oryzae</i> are major diseases responsible for significant yield loss in rice production across all rice growing regions. Host pla...
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| Format: | Article |
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MDPI AG
2019-12-01
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| Series: | Agronomy |
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| Online Access: | https://www.mdpi.com/2073-4395/10/1/42 |
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| author | Samuel C. Chukwu Mohd Y. Rafii Shairul I. Ramlee Siti I. Ismail Yusuff Oladosu Kazeem Kolapo Ibrahim Musa Jamilu Halidu Isma’ila Muhammad Muideen Ahmed |
| author_facet | Samuel C. Chukwu Mohd Y. Rafii Shairul I. Ramlee Siti I. Ismail Yusuff Oladosu Kazeem Kolapo Ibrahim Musa Jamilu Halidu Isma’ila Muhammad Muideen Ahmed |
| author_sort | Samuel C. Chukwu |
| collection | DOAJ |
| description | Bacterial leaf blight caused by <i>Xanthomonas oryzae</i> pv <i>oryzae</i> (<i>Xoo</i>) and blast caused by <i>Magnaporthe oryzae</i> are major diseases responsible for significant yield loss in rice production across all rice growing regions. Host plant resistance has been advocated as a sustainable means of guarding against the diseases. This experiment was conducted with the aim to introgress multiple resistance genes against bacterial leaf blight and blast diseases through marker-assisted backcross breeding. Two dominant (<i>Xa4</i> and <i>Xa21</i>) and two recessive (<i>xa5</i> and <i>xa13</i>) <i>Xoo</i> resistance genes were introgressed into a high yielding Malaysian rice variety Putra-1 with genetic background of three blast resistance (<i>Piz</i>, <i>Pi2</i> and <i>Pi9</i>) genes. Eight polymorphic tightly linked functional and SSR markers were used for foreground selection of target genes. Seventy nine polymorphic SSR markers were used in background selection. The plants were challenged at initial stage of breeding and challenged again at BC<sub>2</sub>F<sub>2</sub> with the most virulent Malaysian pathotypes of <i>Xoo</i> (P7.7) and <i>Magnaporthe oryzae</i> (P7.2) to test their resistance. Results obtained from foreground marker segregation analysis at BC<sub>1</sub>F<sub>1</sub> and BC<sub>2</sub>F<sub>1</sub> showed that the marker polymorphism both fitted into the Mendel’s single gene segregation ratio of 1:1 for both <i>Xoo</i> and blast resistance. At BC<sub>2</sub>F<sub>2</sub>, results indicated that foreground marker polymorphism fitted into the expected Mendelian ratio of 1:2:1 for blast resistance only. Marker-assisted background selection revealed high percentage of recurrent parent genome recovery (95.9%). It was concluded that the inheritance of blast resistance in the introgressed lines was mainly due to single gene action while the inheritance of <i>Xoo</i> resistance was substantially due to single nuclear gene action. The incorporation of four bacterial leaf blight and three blast resistance genes (<i>Xa4 + xa5</i> + <i>xa13</i> + <i>Xa21; Pi9 + Pi2</i> + <i>Piz</i>) in the newly developed lines would provide for broad spectrum and durable resistance against the two major diseases studied. |
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| issn | 2073-4395 |
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| spelling | doaj.art-7750a8882fcb49aa9eb702f67125f1bc2022-12-21T21:30:12ZengMDPI AGAgronomy2073-43952019-12-011014210.3390/agronomy10010042agronomy10010042Marker-Assisted Introgression of Multiple Resistance Genes Confers Broad Spectrum Resistance against Bacterial Leaf Blight and Blast Diseases in PUTRA-1 Rice VarietySamuel C. Chukwu0Mohd Y. Rafii1Shairul I. Ramlee2Siti I. Ismail3Yusuff Oladosu4Kazeem Kolapo5Ibrahim Musa6Jamilu Halidu7Isma’ila Muhammad8Muideen Ahmed9Laboratory of Climate Smart Food Crop Production, Institute of Tropical Agriculture and Food Security, Universiti Putra Malaysia, UPM Serdang, Selangor 43400, MalaysiaLaboratory of Climate Smart Food Crop Production, Institute of Tropical Agriculture and Food Security, Universiti Putra Malaysia, UPM Serdang, Selangor 43400, MalaysiaDepartment of Crop Science, Faculty of Agriculture, Universiti Putra Malaysia, UPM Serdang, Selangor 43400, MalaysiaDepartment of Plant Protection, Faculty of Agriculture, Universiti Putra Malaysia, UPM Serdang, Selangor 43400, MalaysiaInstitute of Tropical Agriculture and Food Security, Universiti Putra Malaysia, UPM Serdang, Selangor 43400, MalaysiaInstitute of Tropical Agriculture and Food Security, Universiti Putra Malaysia, UPM Serdang, Selangor 43400, MalaysiaInstitute of Tropical Agriculture and Food Security, Universiti Putra Malaysia, UPM Serdang, Selangor 43400, MalaysiaInstitute of Tropical Agriculture and Food Security, Universiti Putra Malaysia, UPM Serdang, Selangor 43400, MalaysiaInstitute of Tropical Agriculture and Food Security, Universiti Putra Malaysia, UPM Serdang, Selangor 43400, MalaysiaInstitute of Tropical Agriculture and Food Security, Universiti Putra Malaysia, UPM Serdang, Selangor 43400, MalaysiaBacterial leaf blight caused by <i>Xanthomonas oryzae</i> pv <i>oryzae</i> (<i>Xoo</i>) and blast caused by <i>Magnaporthe oryzae</i> are major diseases responsible for significant yield loss in rice production across all rice growing regions. Host plant resistance has been advocated as a sustainable means of guarding against the diseases. This experiment was conducted with the aim to introgress multiple resistance genes against bacterial leaf blight and blast diseases through marker-assisted backcross breeding. Two dominant (<i>Xa4</i> and <i>Xa21</i>) and two recessive (<i>xa5</i> and <i>xa13</i>) <i>Xoo</i> resistance genes were introgressed into a high yielding Malaysian rice variety Putra-1 with genetic background of three blast resistance (<i>Piz</i>, <i>Pi2</i> and <i>Pi9</i>) genes. Eight polymorphic tightly linked functional and SSR markers were used for foreground selection of target genes. Seventy nine polymorphic SSR markers were used in background selection. The plants were challenged at initial stage of breeding and challenged again at BC<sub>2</sub>F<sub>2</sub> with the most virulent Malaysian pathotypes of <i>Xoo</i> (P7.7) and <i>Magnaporthe oryzae</i> (P7.2) to test their resistance. Results obtained from foreground marker segregation analysis at BC<sub>1</sub>F<sub>1</sub> and BC<sub>2</sub>F<sub>1</sub> showed that the marker polymorphism both fitted into the Mendel’s single gene segregation ratio of 1:1 for both <i>Xoo</i> and blast resistance. At BC<sub>2</sub>F<sub>2</sub>, results indicated that foreground marker polymorphism fitted into the expected Mendelian ratio of 1:2:1 for blast resistance only. Marker-assisted background selection revealed high percentage of recurrent parent genome recovery (95.9%). It was concluded that the inheritance of blast resistance in the introgressed lines was mainly due to single gene action while the inheritance of <i>Xoo</i> resistance was substantially due to single nuclear gene action. The incorporation of four bacterial leaf blight and three blast resistance genes (<i>Xa4 + xa5</i> + <i>xa13</i> + <i>Xa21; Pi9 + Pi2</i> + <i>Piz</i>) in the newly developed lines would provide for broad spectrum and durable resistance against the two major diseases studied.https://www.mdpi.com/2073-4395/10/1/42rice<i>xoo</i><i>magnaporthe oryzae</i>gene actionbackcrossingssr marker |
| spellingShingle | Samuel C. Chukwu Mohd Y. Rafii Shairul I. Ramlee Siti I. Ismail Yusuff Oladosu Kazeem Kolapo Ibrahim Musa Jamilu Halidu Isma’ila Muhammad Muideen Ahmed Marker-Assisted Introgression of Multiple Resistance Genes Confers Broad Spectrum Resistance against Bacterial Leaf Blight and Blast Diseases in PUTRA-1 Rice Variety Agronomy rice <i>xoo</i> <i>magnaporthe oryzae</i> gene action backcrossing ssr marker |
| title | Marker-Assisted Introgression of Multiple Resistance Genes Confers Broad Spectrum Resistance against Bacterial Leaf Blight and Blast Diseases in PUTRA-1 Rice Variety |
| title_full | Marker-Assisted Introgression of Multiple Resistance Genes Confers Broad Spectrum Resistance against Bacterial Leaf Blight and Blast Diseases in PUTRA-1 Rice Variety |
| title_fullStr | Marker-Assisted Introgression of Multiple Resistance Genes Confers Broad Spectrum Resistance against Bacterial Leaf Blight and Blast Diseases in PUTRA-1 Rice Variety |
| title_full_unstemmed | Marker-Assisted Introgression of Multiple Resistance Genes Confers Broad Spectrum Resistance against Bacterial Leaf Blight and Blast Diseases in PUTRA-1 Rice Variety |
| title_short | Marker-Assisted Introgression of Multiple Resistance Genes Confers Broad Spectrum Resistance against Bacterial Leaf Blight and Blast Diseases in PUTRA-1 Rice Variety |
| title_sort | marker assisted introgression of multiple resistance genes confers broad spectrum resistance against bacterial leaf blight and blast diseases in putra 1 rice variety |
| topic | rice <i>xoo</i> <i>magnaporthe oryzae</i> gene action backcrossing ssr marker |
| url | https://www.mdpi.com/2073-4395/10/1/42 |
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