Improved Production of α-Amylase by <i>Aspergillus terreus</i> in Presence of Oxygen-Vector
<i>n</i>-Dodecane has been investigated as an oxygen-vector for improving α-amylase biosynthesis using the strain <i>Aspergillus terreus</i>. In aerobic microbial cultivation, continuous supply of oxygen is required especially due to its low solubility in the growth medium, i...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-06-01
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| Series: | Fermentation |
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| Online Access: | https://www.mdpi.com/2311-5637/8/6/271 |
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| author | Alexandra Cristina Blaga Dan Caşcaval Anca Irina Galaction |
| author_facet | Alexandra Cristina Blaga Dan Caşcaval Anca Irina Galaction |
| author_sort | Alexandra Cristina Blaga |
| collection | DOAJ |
| description | <i>n</i>-Dodecane has been investigated as an oxygen-vector for improving α-amylase biosynthesis using the strain <i>Aspergillus terreus</i>. In aerobic microbial cultivation, continuous supply of oxygen is required especially due to its low solubility in the growth medium, in particular at high viscosity, but the limitations of oxygen mass transfer in these systems can be overcome by the addition of water-insoluble compounds which possess a strong affinity for oxygen, namely oxygen-vectors. The use of <i>n</i>-dodecane (as an oxygen-vector) in the fermentation medium of <i>A. terreus</i> can significantly improve the bioprocess performance and enhance α-amylase production. Using 5% <i>n</i>-dodecane at 35 °C, an increase of 1.8–2 times in the enzymatic activity was recorded. In the oxygen-vector’s absence, the highest amount of biomass was obtained at 35 °C, while in the presence of 5% vol. <i>n</i>-dodecane, the amount of fungal biomass increased by approximately 70%, with a shift in optimum temperature to 40 °C, generating also an enzymatic activity increase of 2.30 times. Moreover, the oxygen-vector’s addition in the fermentation broth influenced the fungal morphological development in the form of larger pellets with a more compact structure compared to the system without <i>n</i>-dodecane, with a positive effect on the fermentation performance (higher α-amylase activity production). |
| first_indexed | 2024-03-09T23:50:43Z |
| format | Article |
| id | doaj.art-77539d95a9054cc5b555396a58b41c95 |
| institution | Directory Open Access Journal |
| issn | 2311-5637 |
| language | English |
| last_indexed | 2024-03-09T23:50:43Z |
| publishDate | 2022-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Fermentation |
| spelling | doaj.art-77539d95a9054cc5b555396a58b41c952023-11-23T16:34:59ZengMDPI AGFermentation2311-56372022-06-018627110.3390/fermentation8060271Improved Production of α-Amylase by <i>Aspergillus terreus</i> in Presence of Oxygen-VectorAlexandra Cristina Blaga0Dan Caşcaval1Anca Irina Galaction2Department of Organic, Biochemical and Food Engineering, Cristofor Simionescu Faculty of Chemical Engineering and Environmental Protection, Gheorghe Asachi Technical University Iasi, 700050 Iasi, RomaniaDepartment of Organic, Biochemical and Food Engineering, Cristofor Simionescu Faculty of Chemical Engineering and Environmental Protection, Gheorghe Asachi Technical University Iasi, 700050 Iasi, RomaniaDepartment of Biomedical Sciences, Faculty of Medical Bioengineering, Grigore T. Popa University of Medicine and Pharmacy, 700454 Iasi, Romania<i>n</i>-Dodecane has been investigated as an oxygen-vector for improving α-amylase biosynthesis using the strain <i>Aspergillus terreus</i>. In aerobic microbial cultivation, continuous supply of oxygen is required especially due to its low solubility in the growth medium, in particular at high viscosity, but the limitations of oxygen mass transfer in these systems can be overcome by the addition of water-insoluble compounds which possess a strong affinity for oxygen, namely oxygen-vectors. The use of <i>n</i>-dodecane (as an oxygen-vector) in the fermentation medium of <i>A. terreus</i> can significantly improve the bioprocess performance and enhance α-amylase production. Using 5% <i>n</i>-dodecane at 35 °C, an increase of 1.8–2 times in the enzymatic activity was recorded. In the oxygen-vector’s absence, the highest amount of biomass was obtained at 35 °C, while in the presence of 5% vol. <i>n</i>-dodecane, the amount of fungal biomass increased by approximately 70%, with a shift in optimum temperature to 40 °C, generating also an enzymatic activity increase of 2.30 times. Moreover, the oxygen-vector’s addition in the fermentation broth influenced the fungal morphological development in the form of larger pellets with a more compact structure compared to the system without <i>n</i>-dodecane, with a positive effect on the fermentation performance (higher α-amylase activity production).https://www.mdpi.com/2311-5637/8/6/271<i>Aspergillus terreus</i>α-amylaseenzymatic activityoxygen-vector |
| spellingShingle | Alexandra Cristina Blaga Dan Caşcaval Anca Irina Galaction Improved Production of α-Amylase by <i>Aspergillus terreus</i> in Presence of Oxygen-Vector Fermentation <i>Aspergillus terreus</i> α-amylase enzymatic activity oxygen-vector |
| title | Improved Production of α-Amylase by <i>Aspergillus terreus</i> in Presence of Oxygen-Vector |
| title_full | Improved Production of α-Amylase by <i>Aspergillus terreus</i> in Presence of Oxygen-Vector |
| title_fullStr | Improved Production of α-Amylase by <i>Aspergillus terreus</i> in Presence of Oxygen-Vector |
| title_full_unstemmed | Improved Production of α-Amylase by <i>Aspergillus terreus</i> in Presence of Oxygen-Vector |
| title_short | Improved Production of α-Amylase by <i>Aspergillus terreus</i> in Presence of Oxygen-Vector |
| title_sort | improved production of α amylase by i aspergillus terreus i in presence of oxygen vector |
| topic | <i>Aspergillus terreus</i> α-amylase enzymatic activity oxygen-vector |
| url | https://www.mdpi.com/2311-5637/8/6/271 |
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