Subcellular Proteomics: Application to Elucidation of Flooding-Response Mechanisms in Soybean
Soybean, which is rich in protein and oil, is cultivated in several climatic zones; however, its growth is markedly decreased by flooding. Proteomics is a useful tool for understanding the flooding-response mechanism in soybean. Subcellular proteomics has the potential to elucidate localized cellula...
| Main Authors: | , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2018-02-01
|
| Series: | Proteomes |
| Subjects: | |
| Online Access: | http://www.mdpi.com/2227-7382/6/1/13 |
| _version_ | 1818035220850409472 |
|---|---|
| author | Setsuko Komatsu Akiko Hashiguchi |
| author_facet | Setsuko Komatsu Akiko Hashiguchi |
| author_sort | Setsuko Komatsu |
| collection | DOAJ |
| description | Soybean, which is rich in protein and oil, is cultivated in several climatic zones; however, its growth is markedly decreased by flooding. Proteomics is a useful tool for understanding the flooding-response mechanism in soybean. Subcellular proteomics has the potential to elucidate localized cellular responses and investigate communications among subcellular components during plant growth and during stress. Under flooding, proteins related to signaling, stress and the antioxidative system are increased in the plasma membrane; scavenging enzymes for reactive-oxygen species are suppressed in the cell wall; protein translation is suppressed through inhibition of proteins related to preribosome biogenesis and mRNA processing in the nucleus; levels of proteins involved in the electron transport chain are reduced in the mitochondrion; and levels of proteins related to protein folding are decreased in the endoplasmic reticulum. This review discusses the advantages of a gel-free/label-free proteomic technique and methods of plant subcellular purification. It also summarizes cellular events in soybean under flooding and discusses future prospects for generation of flooding-tolerant soybean. |
| first_indexed | 2024-12-10T06:51:36Z |
| format | Article |
| id | doaj.art-7757a890000544a29b352fd49ddb19e3 |
| institution | Directory Open Access Journal |
| issn | 2227-7382 |
| language | English |
| last_indexed | 2024-12-10T06:51:36Z |
| publishDate | 2018-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Proteomes |
| spelling | doaj.art-7757a890000544a29b352fd49ddb19e32022-12-22T01:58:32ZengMDPI AGProteomes2227-73822018-02-01611310.3390/proteomes6010013proteomes6010013Subcellular Proteomics: Application to Elucidation of Flooding-Response Mechanisms in SoybeanSetsuko Komatsu0Akiko Hashiguchi1Faculty of Life and Environmental Sciences, University of Tsukuba, Tsukuba 305-8572, JapanFaculty of Medicine, University of Tsukuba, Tsukuba 305-8577, JapanSoybean, which is rich in protein and oil, is cultivated in several climatic zones; however, its growth is markedly decreased by flooding. Proteomics is a useful tool for understanding the flooding-response mechanism in soybean. Subcellular proteomics has the potential to elucidate localized cellular responses and investigate communications among subcellular components during plant growth and during stress. Under flooding, proteins related to signaling, stress and the antioxidative system are increased in the plasma membrane; scavenging enzymes for reactive-oxygen species are suppressed in the cell wall; protein translation is suppressed through inhibition of proteins related to preribosome biogenesis and mRNA processing in the nucleus; levels of proteins involved in the electron transport chain are reduced in the mitochondrion; and levels of proteins related to protein folding are decreased in the endoplasmic reticulum. This review discusses the advantages of a gel-free/label-free proteomic technique and methods of plant subcellular purification. It also summarizes cellular events in soybean under flooding and discusses future prospects for generation of flooding-tolerant soybean.http://www.mdpi.com/2227-7382/6/1/13subcellular proteomicsflooding-response mechanismsoybean |
| spellingShingle | Setsuko Komatsu Akiko Hashiguchi Subcellular Proteomics: Application to Elucidation of Flooding-Response Mechanisms in Soybean Proteomes subcellular proteomics flooding-response mechanism soybean |
| title | Subcellular Proteomics: Application to Elucidation of Flooding-Response Mechanisms in Soybean |
| title_full | Subcellular Proteomics: Application to Elucidation of Flooding-Response Mechanisms in Soybean |
| title_fullStr | Subcellular Proteomics: Application to Elucidation of Flooding-Response Mechanisms in Soybean |
| title_full_unstemmed | Subcellular Proteomics: Application to Elucidation of Flooding-Response Mechanisms in Soybean |
| title_short | Subcellular Proteomics: Application to Elucidation of Flooding-Response Mechanisms in Soybean |
| title_sort | subcellular proteomics application to elucidation of flooding response mechanisms in soybean |
| topic | subcellular proteomics flooding-response mechanism soybean |
| url | http://www.mdpi.com/2227-7382/6/1/13 |
| work_keys_str_mv | AT setsukokomatsu subcellularproteomicsapplicationtoelucidationoffloodingresponsemechanismsinsoybean AT akikohashiguchi subcellularproteomicsapplicationtoelucidationoffloodingresponsemechanismsinsoybean |