Zinc Oxide Nanoparticles Promote YAP/TAZ Nuclear Localization in Alveolar Epithelial Type II Cells
We investigated roles of Hippo signaling pathway components in alveolar type II cells (AECII) after zinc oxide nanoparticle (ZnONP) exposure. ZnONPs physicochemistry was characterized using field emission-scanning electron microscopy (FE-SEM) and energy-dispersive X-ray (EDX) microanalysis. ZnONP de...
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MDPI AG
2022-02-01
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| Series: | Atmosphere |
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| Online Access: | https://www.mdpi.com/2073-4433/13/2/334 |
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| author | Vincent Laiman Didik Setyo Heriyanto Yueh-Lun Lee Ching-Huang Lai Chih-Hong Pan Wei-Liang Chen Chung-Ching Wang Kai-Jen Chuang Jer-Hwa Chang Hsiao-Chi Chuang |
| author_facet | Vincent Laiman Didik Setyo Heriyanto Yueh-Lun Lee Ching-Huang Lai Chih-Hong Pan Wei-Liang Chen Chung-Ching Wang Kai-Jen Chuang Jer-Hwa Chang Hsiao-Chi Chuang |
| author_sort | Vincent Laiman |
| collection | DOAJ |
| description | We investigated roles of Hippo signaling pathway components in alveolar type II cells (AECII) after zinc oxide nanoparticle (ZnONP) exposure. ZnONPs physicochemistry was characterized using field emission-scanning electron microscopy (FE-SEM) and energy-dispersive X-ray (EDX) microanalysis. ZnONP deposition in human respiratory tract was estimated using multiple-path particle dosimetry (MPPD) model. MLE-12 AECII were cultured and exposed to 0, 1, and 5 μg/mL of ZnONPs for 24 h. Western blots were used to investigate signaling pathways associated with Yes-associated protein (YAP)/transcriptional co-activator with PDZ-binding motif (TAZ), cell adherens junctions, differentiation, and senescence. ZnONPs morphology was irregular, with Zn and O identified. Approximately 72% of inhaled ZnONPs were deposited in lungs, with 26% being deposited in alveolar regions. ZnONP exposure increased nuclear YAP expression and decreased cytoplasmic YAP expression by AECII. Adherens junction proteins, E-cadherin, α-catenin, and β-catenin, on AECII decreased after ZnONP exposure. ZnONP exposure of AECII increased alveolar type I (AECI) transition protein, LGALS3, and the AECI protein, T1α, while decreasing AECII SPC expression. ZnONP exposure induced Sirt1 and p53 senescence proteins by AECII. Our findings showed that inhalable ZnONPs can deposit in alveoli, which promotes YAP nuclear localization in AECII, resulting in decrease tight junctions, cell differentiation, and cell senescence. |
| first_indexed | 2024-03-09T22:37:48Z |
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| issn | 2073-4433 |
| language | English |
| last_indexed | 2024-03-09T22:37:48Z |
| publishDate | 2022-02-01 |
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| record_format | Article |
| series | Atmosphere |
| spelling | doaj.art-775b1a2a5e7841af954fa1c809e3da3e2023-11-23T18:46:00ZengMDPI AGAtmosphere2073-44332022-02-0113233410.3390/atmos13020334Zinc Oxide Nanoparticles Promote YAP/TAZ Nuclear Localization in Alveolar Epithelial Type II CellsVincent Laiman0Didik Setyo Heriyanto1Yueh-Lun Lee2Ching-Huang Lai3Chih-Hong Pan4Wei-Liang Chen5Chung-Ching Wang6Kai-Jen Chuang7Jer-Hwa Chang8Hsiao-Chi Chuang9International PhD Program in Medicine, College of Medicine, Taipei Medical University, Taipei 11031, TaiwanDepartment of Anatomical Pathology, Faculty of Medicine, Public Health and Nursing, Universitas Gadjah Mada—Dr. Sardjito Hospital, Yogyakarta 55281, IndonesiaDepartment of Microbiology and Immunology, School of Medicine, College of Medicine, Taipei Medical University, Taipei 11031, TaiwanSchool of Public Health, National Defense Medical Center, Taipei 11490, TaiwanSchool of Public Health, National Defense Medical Center, Taipei 11490, TaiwanDepartment of Family and Community Medicine, Division of Family Medicine, Tri-Service General Hospital, Taipei 11490, TaiwanDepartment of Family and Community Medicine, Division of Family Medicine, Tri-Service General Hospital, Taipei 11490, TaiwanSchool of Public Health, College of Public Health, Taipei Medical University, Taipei 11031, TaiwanSchool of Respiratory Therapy, College of Medicine, Taipei Medical University, Taipei 11031, TaiwanSchool of Respiratory Therapy, College of Medicine, Taipei Medical University, Taipei 11031, TaiwanWe investigated roles of Hippo signaling pathway components in alveolar type II cells (AECII) after zinc oxide nanoparticle (ZnONP) exposure. ZnONPs physicochemistry was characterized using field emission-scanning electron microscopy (FE-SEM) and energy-dispersive X-ray (EDX) microanalysis. ZnONP deposition in human respiratory tract was estimated using multiple-path particle dosimetry (MPPD) model. MLE-12 AECII were cultured and exposed to 0, 1, and 5 μg/mL of ZnONPs for 24 h. Western blots were used to investigate signaling pathways associated with Yes-associated protein (YAP)/transcriptional co-activator with PDZ-binding motif (TAZ), cell adherens junctions, differentiation, and senescence. ZnONPs morphology was irregular, with Zn and O identified. Approximately 72% of inhaled ZnONPs were deposited in lungs, with 26% being deposited in alveolar regions. ZnONP exposure increased nuclear YAP expression and decreased cytoplasmic YAP expression by AECII. Adherens junction proteins, E-cadherin, α-catenin, and β-catenin, on AECII decreased after ZnONP exposure. ZnONP exposure of AECII increased alveolar type I (AECI) transition protein, LGALS3, and the AECI protein, T1α, while decreasing AECII SPC expression. ZnONP exposure induced Sirt1 and p53 senescence proteins by AECII. Our findings showed that inhalable ZnONPs can deposit in alveoli, which promotes YAP nuclear localization in AECII, resulting in decrease tight junctions, cell differentiation, and cell senescence.https://www.mdpi.com/2073-4433/13/2/334Hippo pathwaylung depositionnanoparticlephysicochemistrysenescencewelding fumes |
| spellingShingle | Vincent Laiman Didik Setyo Heriyanto Yueh-Lun Lee Ching-Huang Lai Chih-Hong Pan Wei-Liang Chen Chung-Ching Wang Kai-Jen Chuang Jer-Hwa Chang Hsiao-Chi Chuang Zinc Oxide Nanoparticles Promote YAP/TAZ Nuclear Localization in Alveolar Epithelial Type II Cells Atmosphere Hippo pathway lung deposition nanoparticle physicochemistry senescence welding fumes |
| title | Zinc Oxide Nanoparticles Promote YAP/TAZ Nuclear Localization in Alveolar Epithelial Type II Cells |
| title_full | Zinc Oxide Nanoparticles Promote YAP/TAZ Nuclear Localization in Alveolar Epithelial Type II Cells |
| title_fullStr | Zinc Oxide Nanoparticles Promote YAP/TAZ Nuclear Localization in Alveolar Epithelial Type II Cells |
| title_full_unstemmed | Zinc Oxide Nanoparticles Promote YAP/TAZ Nuclear Localization in Alveolar Epithelial Type II Cells |
| title_short | Zinc Oxide Nanoparticles Promote YAP/TAZ Nuclear Localization in Alveolar Epithelial Type II Cells |
| title_sort | zinc oxide nanoparticles promote yap taz nuclear localization in alveolar epithelial type ii cells |
| topic | Hippo pathway lung deposition nanoparticle physicochemistry senescence welding fumes |
| url | https://www.mdpi.com/2073-4433/13/2/334 |
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