Luteolin sensitizes the antitumor effect of cisplatin in drug-resistant ovarian cancer via induction of apoptosis and inhibition of cell migration and invasion
Abstract Luteolin, a polyphenolic flavone, has been demonstrated to exert anti-tumor activity in various cancer types. Cisplatin drug resistance is a major obstacle in the management of ovarian cancer. In the present study, we investigated the chemo-sensitizing effect of luteolin in both cisplatin-r...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2018-11-01
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| Series: | Journal of Ovarian Research |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s13048-018-0468-y |
| _version_ | 1797968779062280192 |
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| author | Haixia Wang Youjun Luo Tiankui Qiao Zhaoxia Wu Zhonghua Huang |
| author_facet | Haixia Wang Youjun Luo Tiankui Qiao Zhaoxia Wu Zhonghua Huang |
| author_sort | Haixia Wang |
| collection | DOAJ |
| description | Abstract Luteolin, a polyphenolic flavone, has been demonstrated to exert anti-tumor activity in various cancer types. Cisplatin drug resistance is a major obstacle in the management of ovarian cancer. In the present study, we investigated the chemo-sensitizing effect of luteolin in both cisplatin-resistant ovarian cancer cell line and a mice xenotransplant model. In vitro, CCK-8 assay showed that luteolin inhibited cell proliferation in a dose-dependent manner, and luteolin enhanced anti-proliferation effect of cisplatin on cisplatin-resistant ovarian cancer CAOV3/DDP cells. Flow cytometry revealed that luteolin enhanced cell apoptosis in combination with cisplatin. Western blotting and qRT-PCR assay revealed that luteolin increased cisplatin-induced downregulation of Bcl-2 expression. In addition, wound-healing assay and Matrigel invasion assay showed that luteolin and cisplatin synergistically inhibited migration and invasion of CAOV3/DDP cells. Moreover, in vivo, luteolin enhanced cisplatin-induced reduction of tumor growth as well as induction of apoptosis. We suggest that luteolin in combination with cisplatin could potentially be used as a new regimen for the treatment of ovarian cancer. |
| first_indexed | 2024-04-11T02:52:02Z |
| format | Article |
| id | doaj.art-775b2821afd143db97f506207a82ed07 |
| institution | Directory Open Access Journal |
| issn | 1757-2215 |
| language | English |
| last_indexed | 2024-04-11T02:52:02Z |
| publishDate | 2018-11-01 |
| publisher | BMC |
| record_format | Article |
| series | Journal of Ovarian Research |
| spelling | doaj.art-775b2821afd143db97f506207a82ed072023-01-02T16:14:25ZengBMCJournal of Ovarian Research1757-22152018-11-0111111210.1186/s13048-018-0468-yLuteolin sensitizes the antitumor effect of cisplatin in drug-resistant ovarian cancer via induction of apoptosis and inhibition of cell migration and invasionHaixia Wang0Youjun Luo1Tiankui Qiao2Zhaoxia Wu3Zhonghua Huang4Department of Obstetrics and Gynecology, Jinshan branch of Shanghai Sixth People’s Hospital, Shanghai Jiaotong UniversityDepartment of Oncology, Jinshan Hospital, Fudan UniversityDepartment of Oncology, Jinshan Hospital, Fudan UniversityDepartment of Traditional Medicine, Jinshan branch of Shanghai Sixth People’s Hospital, Shanghai Jiaotong UniversityDepartment of Obstetrics and Gynecology, Jinshan branch of Shanghai Sixth People’s Hospital, Shanghai Jiaotong UniversityAbstract Luteolin, a polyphenolic flavone, has been demonstrated to exert anti-tumor activity in various cancer types. Cisplatin drug resistance is a major obstacle in the management of ovarian cancer. In the present study, we investigated the chemo-sensitizing effect of luteolin in both cisplatin-resistant ovarian cancer cell line and a mice xenotransplant model. In vitro, CCK-8 assay showed that luteolin inhibited cell proliferation in a dose-dependent manner, and luteolin enhanced anti-proliferation effect of cisplatin on cisplatin-resistant ovarian cancer CAOV3/DDP cells. Flow cytometry revealed that luteolin enhanced cell apoptosis in combination with cisplatin. Western blotting and qRT-PCR assay revealed that luteolin increased cisplatin-induced downregulation of Bcl-2 expression. In addition, wound-healing assay and Matrigel invasion assay showed that luteolin and cisplatin synergistically inhibited migration and invasion of CAOV3/DDP cells. Moreover, in vivo, luteolin enhanced cisplatin-induced reduction of tumor growth as well as induction of apoptosis. We suggest that luteolin in combination with cisplatin could potentially be used as a new regimen for the treatment of ovarian cancer.http://link.springer.com/article/10.1186/s13048-018-0468-yLuteolinCisplatin-resistant ovarian cancerApoptosisMigrationInvasion |
| spellingShingle | Haixia Wang Youjun Luo Tiankui Qiao Zhaoxia Wu Zhonghua Huang Luteolin sensitizes the antitumor effect of cisplatin in drug-resistant ovarian cancer via induction of apoptosis and inhibition of cell migration and invasion Journal of Ovarian Research Luteolin Cisplatin-resistant ovarian cancer Apoptosis Migration Invasion |
| title | Luteolin sensitizes the antitumor effect of cisplatin in drug-resistant ovarian cancer via induction of apoptosis and inhibition of cell migration and invasion |
| title_full | Luteolin sensitizes the antitumor effect of cisplatin in drug-resistant ovarian cancer via induction of apoptosis and inhibition of cell migration and invasion |
| title_fullStr | Luteolin sensitizes the antitumor effect of cisplatin in drug-resistant ovarian cancer via induction of apoptosis and inhibition of cell migration and invasion |
| title_full_unstemmed | Luteolin sensitizes the antitumor effect of cisplatin in drug-resistant ovarian cancer via induction of apoptosis and inhibition of cell migration and invasion |
| title_short | Luteolin sensitizes the antitumor effect of cisplatin in drug-resistant ovarian cancer via induction of apoptosis and inhibition of cell migration and invasion |
| title_sort | luteolin sensitizes the antitumor effect of cisplatin in drug resistant ovarian cancer via induction of apoptosis and inhibition of cell migration and invasion |
| topic | Luteolin Cisplatin-resistant ovarian cancer Apoptosis Migration Invasion |
| url | http://link.springer.com/article/10.1186/s13048-018-0468-y |
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