Rapid identification of Trichoderma koningiopsis and Trichoderma longibrachiatum using sequence-characterized amplified region markers
Abstract Molecular markers enable the detection and classification of fungi isolated from their natural environments. To develop species-specific markers for detecting Trichoderma koningiopsis and T. longibrachiatum, the sequence-characterized amplified region technique, using 20 inter-simple sequen...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
| Published: |
SpringerOpen
2019-03-01
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| Series: | Egyptian Journal of Biological Pest Control |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s41938-019-0113-0 |
| _version_ | 1819276547409838080 |
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| author | Mohamed M. Hassan Mona A. Farid Ahmed Gaber |
| author_facet | Mohamed M. Hassan Mona A. Farid Ahmed Gaber |
| author_sort | Mohamed M. Hassan |
| collection | DOAJ |
| description | Abstract Molecular markers enable the detection and classification of fungi isolated from their natural environments. To develop species-specific markers for detecting Trichoderma koningiopsis and T. longibrachiatum, the sequence-characterized amplified region technique, using 20 inter-simple sequence repeat-polymerase chain reaction primers, was performed. The two specific markers for amplifying a single unique band consistent with T. koningiopsis and T. longibrachiatum, which were absent with other Trichoderma strains, were successfully identified. These fragments had no meaningful sequence homology with known sequences available in the National Center for Biotechnology Information and TrichOKEY databases. Compared with traditional identification techniques, these markers can facilitate more rapid and less complicated studies of Trichoderma population dynamics and evaluate their establishment after release into agricultural environments. |
| first_indexed | 2024-12-23T23:41:57Z |
| format | Article |
| id | doaj.art-775d026a4e4c41f48d474d0f602e9b2b |
| institution | Directory Open Access Journal |
| issn | 2536-9342 |
| language | English |
| last_indexed | 2024-12-23T23:41:57Z |
| publishDate | 2019-03-01 |
| publisher | SpringerOpen |
| record_format | Article |
| series | Egyptian Journal of Biological Pest Control |
| spelling | doaj.art-775d026a4e4c41f48d474d0f602e9b2b2022-12-21T17:25:38ZengSpringerOpenEgyptian Journal of Biological Pest Control2536-93422019-03-012911810.1186/s41938-019-0113-0Rapid identification of Trichoderma koningiopsis and Trichoderma longibrachiatum using sequence-characterized amplified region markersMohamed M. Hassan0Mona A. Farid1Ahmed Gaber2Department of Biology, Faculty of Science, Taif UniversityGenetics Department, Faculty of Agriculture, Kafrelsheikh UniversityDepartment of Biology, Faculty of Science, Taif UniversityAbstract Molecular markers enable the detection and classification of fungi isolated from their natural environments. To develop species-specific markers for detecting Trichoderma koningiopsis and T. longibrachiatum, the sequence-characterized amplified region technique, using 20 inter-simple sequence repeat-polymerase chain reaction primers, was performed. The two specific markers for amplifying a single unique band consistent with T. koningiopsis and T. longibrachiatum, which were absent with other Trichoderma strains, were successfully identified. These fragments had no meaningful sequence homology with known sequences available in the National Center for Biotechnology Information and TrichOKEY databases. Compared with traditional identification techniques, these markers can facilitate more rapid and less complicated studies of Trichoderma population dynamics and evaluate their establishment after release into agricultural environments.http://link.springer.com/article/10.1186/s41938-019-0113-0Trichoderma spp.Rapid identificationITS region sequenceISSR-PCRSCAR marker |
| spellingShingle | Mohamed M. Hassan Mona A. Farid Ahmed Gaber Rapid identification of Trichoderma koningiopsis and Trichoderma longibrachiatum using sequence-characterized amplified region markers Egyptian Journal of Biological Pest Control Trichoderma spp. Rapid identification ITS region sequence ISSR-PCR SCAR marker |
| title | Rapid identification of Trichoderma koningiopsis and Trichoderma longibrachiatum using sequence-characterized amplified region markers |
| title_full | Rapid identification of Trichoderma koningiopsis and Trichoderma longibrachiatum using sequence-characterized amplified region markers |
| title_fullStr | Rapid identification of Trichoderma koningiopsis and Trichoderma longibrachiatum using sequence-characterized amplified region markers |
| title_full_unstemmed | Rapid identification of Trichoderma koningiopsis and Trichoderma longibrachiatum using sequence-characterized amplified region markers |
| title_short | Rapid identification of Trichoderma koningiopsis and Trichoderma longibrachiatum using sequence-characterized amplified region markers |
| title_sort | rapid identification of trichoderma koningiopsis and trichoderma longibrachiatum using sequence characterized amplified region markers |
| topic | Trichoderma spp. Rapid identification ITS region sequence ISSR-PCR SCAR marker |
| url | http://link.springer.com/article/10.1186/s41938-019-0113-0 |
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