Combined postconditioning with ischemia and cyclosporine-A restore oxidative stress and histopathological changes in reperfusion injury of diabetic myocardium
Objective(s): Chronic diabetes impedes cardioprotection in reperfusion injury and hence protecting the diabetic heart would have important outcomes. In this study, we evaluated whether combined postconditioning with ischemia and cyclosporine-A can restore oxidative stress and histopathological chang...
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| Format: | Article |
| Language: | English |
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Mashhad University of Medical Sciences
2017-10-01
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| Series: | Iranian Journal of Basic Medical Sciences |
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| Online Access: | http://ijbms.mums.ac.ir/article_9444_8483e9444660ac4282d841c42735fd26.pdf |
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| author | Reza Badalzadeh Seyed Mahmoud Tabatabaei Mustafa Mohammadi Arash Khaki Dariush Mohammadnezhad |
| author_facet | Reza Badalzadeh Seyed Mahmoud Tabatabaei Mustafa Mohammadi Arash Khaki Dariush Mohammadnezhad |
| author_sort | Reza Badalzadeh |
| collection | DOAJ |
| description | Objective(s): Chronic diabetes impedes cardioprotection in reperfusion injury and hence protecting the diabetic heart would have important outcomes. In this study, we evaluated whether combined postconditioning with ischemia and cyclosporine-A can restore oxidative stress and histopathological changes in reperfusion injury of the diabetic myocardium. Materials and Methods: Streptozocin-induced diabetic rats’ hearts and nondiabetic controls in eight subgroups (with or without receiving ischemic-postconditioning (IPostC), cyclosporine-A, an inhibitor of mitochondrial permeability transition, or both of them) suffered from 30 min regional ischemia followed by 45 min reperfusion on an isolated-heart Langendorff system. The levels of lactate dehydrogenase (LDH) in the coronary effluent, and the levels of oxidative stress markers including 8-isoprostane, superoxide dismutase (SOD), glutathione peroxidase (GPX), and total antioxidant capacity (TAC) in myocardial supernatant prepared from the ischemic zone were measured using specific kits, spectrophotometrically. Histopathological studies were performed through the hematoxylin-eosin staining method. Results: Administration of IPostC and cyclosporine-A (alone or together) in nondiabetic hearts potentially reduced the severity of histological changes and level of LDH release as compared with untreated-controls (P0.1). However, the combined postconditioning with ischemia and CsA exerted significant protective effects in diabetic hearts (P |
| first_indexed | 2024-12-14T01:24:09Z |
| format | Article |
| id | doaj.art-775d3d92c23347238426ae5819eef8c9 |
| institution | Directory Open Access Journal |
| issn | 2008-3866 2008-3874 |
| language | English |
| last_indexed | 2024-12-14T01:24:09Z |
| publishDate | 2017-10-01 |
| publisher | Mashhad University of Medical Sciences |
| record_format | Article |
| series | Iranian Journal of Basic Medical Sciences |
| spelling | doaj.art-775d3d92c23347238426ae5819eef8c92022-12-21T23:22:16ZengMashhad University of Medical SciencesIranian Journal of Basic Medical Sciences2008-38662008-38742017-10-0120101079108710.22038/ijbms.2017.94449444Combined postconditioning with ischemia and cyclosporine-A restore oxidative stress and histopathological changes in reperfusion injury of diabetic myocardiumReza Badalzadeh0Seyed Mahmoud Tabatabaei1Mustafa Mohammadi2Arash Khaki3Dariush Mohammadnezhad4Molecular Medicine Research Center, Tabriz University of Medical Sciences, Tabriz, Iran|Drug Applied Research Center, Tabriz University of Medical Sciences, Tabriz, Iran|Cardiovascular Research Center, Tabriz University of Medical Sciences, Tabriz, IranDepartment of Physiology, Tabriz Branch, Islamic Azad University, Tabriz, IranMolecular Medicine Research Center, Tabriz University of Medical Sciences, Tabriz, IranDepartment of Pathology, Tabriz Branch, Islamic Azad University, Tabriz, IranMolecular Medicine Research Center, Tabriz University of Medical Sciences, Tabriz, IranObjective(s): Chronic diabetes impedes cardioprotection in reperfusion injury and hence protecting the diabetic heart would have important outcomes. In this study, we evaluated whether combined postconditioning with ischemia and cyclosporine-A can restore oxidative stress and histopathological changes in reperfusion injury of the diabetic myocardium. Materials and Methods: Streptozocin-induced diabetic rats’ hearts and nondiabetic controls in eight subgroups (with or without receiving ischemic-postconditioning (IPostC), cyclosporine-A, an inhibitor of mitochondrial permeability transition, or both of them) suffered from 30 min regional ischemia followed by 45 min reperfusion on an isolated-heart Langendorff system. The levels of lactate dehydrogenase (LDH) in the coronary effluent, and the levels of oxidative stress markers including 8-isoprostane, superoxide dismutase (SOD), glutathione peroxidase (GPX), and total antioxidant capacity (TAC) in myocardial supernatant prepared from the ischemic zone were measured using specific kits, spectrophotometrically. Histopathological studies were performed through the hematoxylin-eosin staining method. Results: Administration of IPostC and cyclosporine-A (alone or together) in nondiabetic hearts potentially reduced the severity of histological changes and level of LDH release as compared with untreated-controls (P0.1). However, the combined postconditioning with ischemia and CsA exerted significant protective effects in diabetic hearts (Phttp://ijbms.mums.ac.ir/article_9444_8483e9444660ac4282d841c42735fd26.pdfCyclosporine ADiabetesOxidative stressPostconditioningReperfusion injury |
| spellingShingle | Reza Badalzadeh Seyed Mahmoud Tabatabaei Mustafa Mohammadi Arash Khaki Dariush Mohammadnezhad Combined postconditioning with ischemia and cyclosporine-A restore oxidative stress and histopathological changes in reperfusion injury of diabetic myocardium Iranian Journal of Basic Medical Sciences Cyclosporine A Diabetes Oxidative stress Postconditioning Reperfusion injury |
| title | Combined postconditioning with ischemia and cyclosporine-A restore oxidative stress and histopathological changes in reperfusion injury of diabetic myocardium |
| title_full | Combined postconditioning with ischemia and cyclosporine-A restore oxidative stress and histopathological changes in reperfusion injury of diabetic myocardium |
| title_fullStr | Combined postconditioning with ischemia and cyclosporine-A restore oxidative stress and histopathological changes in reperfusion injury of diabetic myocardium |
| title_full_unstemmed | Combined postconditioning with ischemia and cyclosporine-A restore oxidative stress and histopathological changes in reperfusion injury of diabetic myocardium |
| title_short | Combined postconditioning with ischemia and cyclosporine-A restore oxidative stress and histopathological changes in reperfusion injury of diabetic myocardium |
| title_sort | combined postconditioning with ischemia and cyclosporine a restore oxidative stress and histopathological changes in reperfusion injury of diabetic myocardium |
| topic | Cyclosporine A Diabetes Oxidative stress Postconditioning Reperfusion injury |
| url | http://ijbms.mums.ac.ir/article_9444_8483e9444660ac4282d841c42735fd26.pdf |
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