Culex quinquefasciatus vitellogenesis: morphological and biochemical aspects
The vitellogenic process in Culex quinquefasciatus, which is triggered by a blood meal, involves the synthesis, distribution and storage of the nutrients necessary for embryo development. The fat body of an adult female Cx. quinquefasciatus revealed two cell types: large trophocytes and small, eosin...
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| Format: | Article |
| Language: | English |
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Fundação Oswaldo Cruz (FIOCRUZ)
2010-05-01
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| Series: | Memorias do Instituto Oswaldo Cruz |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762010000300003 |
| _version_ | 1797756708727029760 |
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| author | André F Cardoso Renato L Cres Alexandre S Moura Fábio de Almeida A Tania Bijovsky |
| author_facet | André F Cardoso Renato L Cres Alexandre S Moura Fábio de Almeida A Tania Bijovsky |
| author_sort | André F Cardoso |
| collection | DOAJ |
| description | The vitellogenic process in Culex quinquefasciatus, which is triggered by a blood meal, involves the synthesis, distribution and storage of the nutrients necessary for embryo development. The fat body of an adult female Cx. quinquefasciatus revealed two cell types: large trophocytes and small, eosinophilic, "oenocyte-like" cells, which show no morphological changes throughout the gonotrophic cycle. Trophocytes, which only begin to synthesise vitellogenin (Vg) 12 h post-blood meal (PBM), undergo a series of morphological changes following engorgement. These changes include the expansion of the rough endoplasmic reticulum (RER) and Golgi complex, which are later destroyed by autophagosomes. At 84 h PBM, trophocytes return to their pre-engorgement morphology. The ovarian follicles of non-blood-fed Cx. quinquefasciatus contain a cluster of eight undifferentiated cells surrounded by follicular epithelium. After engorgement, the oocyte membrane facing the perioocytic space increases its absorptive surface by microvilli development; large amounts of Vg and lipids are stored between 24 and 48 h PBM. Along with yolk storage in the oocyte, follicular cells exhibit the development of RER cisternae and electron-dense granules begin to fill the perioocytic space, possibly giving rise to endochorion. Later in the gonotrophic cycle, electron-dense vesicles, which are possible exochorion precursors, fuse at the apical membrane of follicular cells. This fusion is followed by follicular cell degeneration. |
| first_indexed | 2024-03-12T18:05:24Z |
| format | Article |
| id | doaj.art-775d7d67f110437180dbbd25459febe4 |
| institution | Directory Open Access Journal |
| issn | 0074-0276 1678-8060 |
| language | English |
| last_indexed | 2024-03-12T18:05:24Z |
| publishDate | 2010-05-01 |
| publisher | Fundação Oswaldo Cruz (FIOCRUZ) |
| record_format | Article |
| series | Memorias do Instituto Oswaldo Cruz |
| spelling | doaj.art-775d7d67f110437180dbbd25459febe42023-08-02T09:28:30ZengFundação Oswaldo Cruz (FIOCRUZ)Memorias do Instituto Oswaldo Cruz0074-02761678-80602010-05-01105325426210.1590/S0074-02762010000300003Culex quinquefasciatus vitellogenesis: morphological and biochemical aspectsAndré F CardosoRenato L CresAlexandre S MouraFábio de AlmeidaA Tania BijovskyThe vitellogenic process in Culex quinquefasciatus, which is triggered by a blood meal, involves the synthesis, distribution and storage of the nutrients necessary for embryo development. The fat body of an adult female Cx. quinquefasciatus revealed two cell types: large trophocytes and small, eosinophilic, "oenocyte-like" cells, which show no morphological changes throughout the gonotrophic cycle. Trophocytes, which only begin to synthesise vitellogenin (Vg) 12 h post-blood meal (PBM), undergo a series of morphological changes following engorgement. These changes include the expansion of the rough endoplasmic reticulum (RER) and Golgi complex, which are later destroyed by autophagosomes. At 84 h PBM, trophocytes return to their pre-engorgement morphology. The ovarian follicles of non-blood-fed Cx. quinquefasciatus contain a cluster of eight undifferentiated cells surrounded by follicular epithelium. After engorgement, the oocyte membrane facing the perioocytic space increases its absorptive surface by microvilli development; large amounts of Vg and lipids are stored between 24 and 48 h PBM. Along with yolk storage in the oocyte, follicular cells exhibit the development of RER cisternae and electron-dense granules begin to fill the perioocytic space, possibly giving rise to endochorion. Later in the gonotrophic cycle, electron-dense vesicles, which are possible exochorion precursors, fuse at the apical membrane of follicular cells. This fusion is followed by follicular cell degeneration.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762010000300003Culex quinquefasciatusvitellogenesisimmunolabellingmorphologyfat bodyovary |
| spellingShingle | André F Cardoso Renato L Cres Alexandre S Moura Fábio de Almeida A Tania Bijovsky Culex quinquefasciatus vitellogenesis: morphological and biochemical aspects Memorias do Instituto Oswaldo Cruz Culex quinquefasciatus vitellogenesis immunolabelling morphology fat body ovary |
| title | Culex quinquefasciatus vitellogenesis: morphological and biochemical aspects |
| title_full | Culex quinquefasciatus vitellogenesis: morphological and biochemical aspects |
| title_fullStr | Culex quinquefasciatus vitellogenesis: morphological and biochemical aspects |
| title_full_unstemmed | Culex quinquefasciatus vitellogenesis: morphological and biochemical aspects |
| title_short | Culex quinquefasciatus vitellogenesis: morphological and biochemical aspects |
| title_sort | culex quinquefasciatus vitellogenesis morphological and biochemical aspects |
| topic | Culex quinquefasciatus vitellogenesis immunolabelling morphology fat body ovary |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762010000300003 |
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