Genome-wide identification, and characterization of the CDPK gene family reveal their involvement in abiotic stress response in Fragaria x ananassa
Abstract Calcium-dependent protein kinases (CDPKs) are encoded by a large gene family and play important roles against biotic and abiotic stresses and in plant growth and development. To date, little is known about the CDPK genes in strawberry (Fragaria x ananassa). In this study, analysis of Fragar...
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2020-07-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-020-67957-9 |
| _version_ | 1818840329432858624 |
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| author | Rosane Lopes Crizel Ellen Cristina Perin Isabel Lopes Vighi Rafael Woloski Amilton Seixas Luciano da Silva Pinto César Valmor Rombaldi Vanessa Galli |
| author_facet | Rosane Lopes Crizel Ellen Cristina Perin Isabel Lopes Vighi Rafael Woloski Amilton Seixas Luciano da Silva Pinto César Valmor Rombaldi Vanessa Galli |
| author_sort | Rosane Lopes Crizel |
| collection | DOAJ |
| description | Abstract Calcium-dependent protein kinases (CDPKs) are encoded by a large gene family and play important roles against biotic and abiotic stresses and in plant growth and development. To date, little is known about the CDPK genes in strawberry (Fragaria x ananassa). In this study, analysis of Fragaria x ananassa CDPK gene family was performed, including gene structures, phylogeny, interactome and expression profiles. Nine new CDPK genes in Fragaria x ananassa were identified based on RNA-seq data. These identified strawberry FaCDPK genes were classified into four main groups, based on the phylogenetic analysis and structural features. FaCDPK genes were differentially expressed during fruit development and ripening, as well as in response to abiotic stress (salt and drought), and hormone (abscisic acid) treatment. In addition, the interaction network analysis pointed out proteins involved in the ABA-dependent response to plant stress via Ca2+ signaling, especially RBOHs. To our knowledge, this is the first report on CDPK families in Fragaria x ananassa, and it will provide valuable information for development of biofortified fruits and stress tolerant plants. |
| first_indexed | 2024-12-19T04:08:27Z |
| format | Article |
| id | doaj.art-775fae9719234c8bb0e8b53c71a30516 |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-12-19T04:08:27Z |
| publishDate | 2020-07-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-775fae9719234c8bb0e8b53c71a305162022-12-21T20:36:29ZengNature PortfolioScientific Reports2045-23222020-07-0110111710.1038/s41598-020-67957-9Genome-wide identification, and characterization of the CDPK gene family reveal their involvement in abiotic stress response in Fragaria x ananassaRosane Lopes Crizel0Ellen Cristina Perin1Isabel Lopes Vighi2Rafael Woloski3Amilton Seixas4Luciano da Silva Pinto5César Valmor Rombaldi6Vanessa Galli7Departamento de Ciência e Tecnologia Agroindustrial, Universidade Federal de PelotasPrograma de Pós-Graduação em Tecnologia de Processos Químicos e Bioquímicos, Universidade Tecnologia Federal do ParanáCentro de Desenvolvimento Tecnológico, Universidade Federal de PelotasCentro de Desenvolvimento Tecnológico, Universidade Federal de PelotasCentro de Desenvolvimento Tecnológico, Universidade Federal de PelotasCentro de Desenvolvimento Tecnológico, Universidade Federal de PelotasDepartamento de Ciência e Tecnologia Agroindustrial, Universidade Federal de PelotasDepartamento de Ciência e Tecnologia Agroindustrial, Universidade Federal de PelotasAbstract Calcium-dependent protein kinases (CDPKs) are encoded by a large gene family and play important roles against biotic and abiotic stresses and in plant growth and development. To date, little is known about the CDPK genes in strawberry (Fragaria x ananassa). In this study, analysis of Fragaria x ananassa CDPK gene family was performed, including gene structures, phylogeny, interactome and expression profiles. Nine new CDPK genes in Fragaria x ananassa were identified based on RNA-seq data. These identified strawberry FaCDPK genes were classified into four main groups, based on the phylogenetic analysis and structural features. FaCDPK genes were differentially expressed during fruit development and ripening, as well as in response to abiotic stress (salt and drought), and hormone (abscisic acid) treatment. In addition, the interaction network analysis pointed out proteins involved in the ABA-dependent response to plant stress via Ca2+ signaling, especially RBOHs. To our knowledge, this is the first report on CDPK families in Fragaria x ananassa, and it will provide valuable information for development of biofortified fruits and stress tolerant plants.https://doi.org/10.1038/s41598-020-67957-9 |
| spellingShingle | Rosane Lopes Crizel Ellen Cristina Perin Isabel Lopes Vighi Rafael Woloski Amilton Seixas Luciano da Silva Pinto César Valmor Rombaldi Vanessa Galli Genome-wide identification, and characterization of the CDPK gene family reveal their involvement in abiotic stress response in Fragaria x ananassa Scientific Reports |
| title | Genome-wide identification, and characterization of the CDPK gene family reveal their involvement in abiotic stress response in Fragaria x ananassa |
| title_full | Genome-wide identification, and characterization of the CDPK gene family reveal their involvement in abiotic stress response in Fragaria x ananassa |
| title_fullStr | Genome-wide identification, and characterization of the CDPK gene family reveal their involvement in abiotic stress response in Fragaria x ananassa |
| title_full_unstemmed | Genome-wide identification, and characterization of the CDPK gene family reveal their involvement in abiotic stress response in Fragaria x ananassa |
| title_short | Genome-wide identification, and characterization of the CDPK gene family reveal their involvement in abiotic stress response in Fragaria x ananassa |
| title_sort | genome wide identification and characterization of the cdpk gene family reveal their involvement in abiotic stress response in fragaria x ananassa |
| url | https://doi.org/10.1038/s41598-020-67957-9 |
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