Determination of azithromycin heteroresistant Campylobacter jejuni in traveler’s diarrhea
Abstract Campylobacter is the most common cause of traveler’s diarrhea (TD) and human bacterial gastroenteritis. A heteroresistant Campylobacter jejuni (C. jejuni) isolate, identified by microbiological methods and characterized with molecular techniques, was obtained from a traveler in Nepal suffer...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2019-05-01
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| Series: | Gut Pathogens |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s13099-019-0301-1 |
| _version_ | 1818109312563675136 |
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| author | Woradee Lurchachaiwong Supaporn Ruksasiri Patcharawalai Wassanarungroj Oralak Serichantalergs Ladaporn Bodhidatta John Crawford Sanjaya Kumar Shrestha Prativa Pandey |
| author_facet | Woradee Lurchachaiwong Supaporn Ruksasiri Patcharawalai Wassanarungroj Oralak Serichantalergs Ladaporn Bodhidatta John Crawford Sanjaya Kumar Shrestha Prativa Pandey |
| author_sort | Woradee Lurchachaiwong |
| collection | DOAJ |
| description | Abstract Campylobacter is the most common cause of traveler’s diarrhea (TD) and human bacterial gastroenteritis. A heteroresistant Campylobacter jejuni (C. jejuni) isolate, identified by microbiological methods and characterized with molecular techniques, was obtained from a traveler in Nepal suffering TD. The presence of atypical colonies within the clear zone of inhibition was the first evidence of an atypical phenotype, leading to additional characterization of this heteroresistant strain. Antimicrobial susceptibility testing (AST) and population analysis profiling (PAP) demonstrated heteroresistance to azithromycin (AZM), a first-line antibiotic treatment for Campylobacter infections. Molecular analysis indicated a point mutation occurred on the 23S rRNA gene at the A2075G transitions, and the number of mutated gene copies was proportional to AZM resistance. Heteroresistant C. jejuni subpopulations from acute TD are likely underestimated, which may lead to treatment failures, as was the case for this patient. The presence of a heteroresistant strain in a high antibiotic environment may select for additional drug resistance and enable distribution into hospital and local communities. |
| first_indexed | 2024-12-11T02:29:15Z |
| format | Article |
| id | doaj.art-776132ba230144cb86bdbe2dd022bb2b |
| institution | Directory Open Access Journal |
| issn | 1757-4749 |
| language | English |
| last_indexed | 2024-12-11T02:29:15Z |
| publishDate | 2019-05-01 |
| publisher | BMC |
| record_format | Article |
| series | Gut Pathogens |
| spelling | doaj.art-776132ba230144cb86bdbe2dd022bb2b2022-12-22T01:23:52ZengBMCGut Pathogens1757-47492019-05-011111510.1186/s13099-019-0301-1Determination of azithromycin heteroresistant Campylobacter jejuni in traveler’s diarrheaWoradee Lurchachaiwong0Supaporn Ruksasiri1Patcharawalai Wassanarungroj2Oralak Serichantalergs3Ladaporn Bodhidatta4John Crawford5Sanjaya Kumar Shrestha6Prativa Pandey7Department of Bacterial and Parasitic Diseases, Armed Forces Research Institute of Medical SciencesDepartment of Bacterial and Parasitic Diseases, Armed Forces Research Institute of Medical SciencesDepartment of Bacterial and Parasitic Diseases, Armed Forces Research Institute of Medical SciencesDepartment of Bacterial and Parasitic Diseases, Armed Forces Research Institute of Medical SciencesDepartment of Bacterial and Parasitic Diseases, Armed Forces Research Institute of Medical SciencesDepartment of Bacterial and Parasitic Diseases, Armed Forces Research Institute of Medical SciencesWalter Reed AFRIMS Research UnitCIWEC Clinic Travel Medicine CenterAbstract Campylobacter is the most common cause of traveler’s diarrhea (TD) and human bacterial gastroenteritis. A heteroresistant Campylobacter jejuni (C. jejuni) isolate, identified by microbiological methods and characterized with molecular techniques, was obtained from a traveler in Nepal suffering TD. The presence of atypical colonies within the clear zone of inhibition was the first evidence of an atypical phenotype, leading to additional characterization of this heteroresistant strain. Antimicrobial susceptibility testing (AST) and population analysis profiling (PAP) demonstrated heteroresistance to azithromycin (AZM), a first-line antibiotic treatment for Campylobacter infections. Molecular analysis indicated a point mutation occurred on the 23S rRNA gene at the A2075G transitions, and the number of mutated gene copies was proportional to AZM resistance. Heteroresistant C. jejuni subpopulations from acute TD are likely underestimated, which may lead to treatment failures, as was the case for this patient. The presence of a heteroresistant strain in a high antibiotic environment may select for additional drug resistance and enable distribution into hospital and local communities.http://link.springer.com/article/10.1186/s13099-019-0301-1HeteroresistanceCampylobacter jejuniAzithromycinTravelers |
| spellingShingle | Woradee Lurchachaiwong Supaporn Ruksasiri Patcharawalai Wassanarungroj Oralak Serichantalergs Ladaporn Bodhidatta John Crawford Sanjaya Kumar Shrestha Prativa Pandey Determination of azithromycin heteroresistant Campylobacter jejuni in traveler’s diarrhea Gut Pathogens Heteroresistance Campylobacter jejuni Azithromycin Travelers |
| title | Determination of azithromycin heteroresistant Campylobacter jejuni in traveler’s diarrhea |
| title_full | Determination of azithromycin heteroresistant Campylobacter jejuni in traveler’s diarrhea |
| title_fullStr | Determination of azithromycin heteroresistant Campylobacter jejuni in traveler’s diarrhea |
| title_full_unstemmed | Determination of azithromycin heteroresistant Campylobacter jejuni in traveler’s diarrhea |
| title_short | Determination of azithromycin heteroresistant Campylobacter jejuni in traveler’s diarrhea |
| title_sort | determination of azithromycin heteroresistant campylobacter jejuni in traveler s diarrhea |
| topic | Heteroresistance Campylobacter jejuni Azithromycin Travelers |
| url | http://link.springer.com/article/10.1186/s13099-019-0301-1 |
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