<b>Evaluation of baicalin in <i>Scutellaria baicalensis georgi</i> using HPLC method</b>
High-performance liquid chromatography (HPLC) has been evaluated for the determination of components in <i>Scutellaria baicalensis georgi</i>, primarily by the separation of baicalin. The samples were extracted at reflux by methanol, and the content of chlorogenic acid was analyzed by HP...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
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Chemical Society of Ethiopia
2010-04-01
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| Series: | Bulletin of the Chemical Society of Ethiopia |
| Subjects: | |
| Online Access: | http://ajol.info/index.php/bcse/article/view/52967 |
| Summary: | High-performance liquid chromatography (HPLC) has been evaluated for the determination of components in <i>Scutellaria baicalensis georgi</i>, primarily by the separation of baicalin. The samples were extracted at reflux by methanol, and the content of chlorogenic acid was analyzed by HPLC. A Diamonsil C18 column (4.6 mm x 150 mm, 5 μm) was used as the analytical column. The mobile phase was methanol, water and phosphoric acid (47:53:0.2). The detection wavelength was 280 nm to determine the content of baicalin. The baicalin showed linearity over the range of 0.12-1.2 μg. Its average recovery was 98.6% and RSD was 0.78%. |
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| ISSN: | 1011-3924 1726-801X |