Magnetic resonance imaging at 7.0 T for evaluation of early lesions of epiphyseal plate and epiphyseal end in a rat model of Kashin-Beck disease
Abstract Background Kashin–Beck disease (KBD) is a disabling osteoarticular disease involving growth and joint cartilage. Early diagnosis can effectively prevent the progress of the disease. However, the early diagnosis of it is still very difficult. Our aim was to study the knee joint lesions of a...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
BMC
2020-08-01
|
| Series: | BMC Musculoskeletal Disorders |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s12891-020-03559-w |
| _version_ | 1818476709403426816 |
|---|---|
| author | Yong Li Pengde Kang Zongke Zhou Fuxing Pei Qing He Dike Ruan |
| author_facet | Yong Li Pengde Kang Zongke Zhou Fuxing Pei Qing He Dike Ruan |
| author_sort | Yong Li |
| collection | DOAJ |
| description | Abstract Background Kashin–Beck disease (KBD) is a disabling osteoarticular disease involving growth and joint cartilage. Early diagnosis can effectively prevent the progress of the disease. However, the early diagnosis of it is still very difficult. Our aim was to study the knee joint lesions of a rat KBD model using ultra-high field magnetic resonance imaging (MRI) and compare it with X-ray imaging to analyze the possible MRI manifestations of KBD, and to further explore ways to determine the pathological damage of KBD in the early stage. Methods A total of 96 Wistar rats were selected and randomly divided into 4 groups: normal diet (Group A), KBD-affected diet (Group B), normal diet+T-2 toxin (Group C), and KBD-affected diet+T-2 toxin (Group D). T-2 toxin was administered at a dose of 0.1 mg/kg/day. In the 4th week, 8th week, and 12th week, eight rats randomly selected in each group were sacrificed by cervical dislocation after undergoing X-ray and 7.0 T MRI imaging, and then knee joints were harvested, sliced, and subjected to hematoxylin-eosin (H&E) staining. Results Characteristic image changes including of continuity interruption and early closure and fusion of epiphyseal plates were observed on T1WI in rat model of KBD. The total necrosis rates in the H&E stain of group A to group D were 4.35, 52.38, 33.3, and 73.68%, respectively. The positive rate of image change under 7.0 T MRI was 0.833 VS. that under X-ray was 0.33 (P = 0.001). Conclusions MRI at 7.0 T is highly sensitive to the early pathological changes of the epiphysis, epiphyseal plate, and metaphyseal end, which can improve imaging positive rate of KBD and decrease the rate of missed diagnosis. This imaging modality can be used for research on early joint lesions and for early diagnosis of KBD. |
| first_indexed | 2024-12-10T09:28:47Z |
| format | Article |
| id | doaj.art-77659dfc1da14eb28c79004ad94b31af |
| institution | Directory Open Access Journal |
| issn | 1471-2474 |
| language | English |
| last_indexed | 2024-12-10T09:28:47Z |
| publishDate | 2020-08-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Musculoskeletal Disorders |
| spelling | doaj.art-77659dfc1da14eb28c79004ad94b31af2022-12-22T01:54:26ZengBMCBMC Musculoskeletal Disorders1471-24742020-08-012111910.1186/s12891-020-03559-wMagnetic resonance imaging at 7.0 T for evaluation of early lesions of epiphyseal plate and epiphyseal end in a rat model of Kashin-Beck diseaseYong Li0Pengde Kang1Zongke Zhou2Fuxing Pei3Qing He4Dike Ruan5Department of Orthopedic Surgery, Sixth Medical Center of Chinese PLA General HospitalDepartment of Orthopedic Surgery, West China Hospital, Sichuan UniversityDepartment of Orthopedic Surgery, West China Hospital, Sichuan UniversityDepartment of Orthopedic Surgery, Sixth Medical Center of Chinese PLA General HospitalDepartment of Orthopedic Surgery, Sixth Medical Center of Chinese PLA General HospitalDepartment of Orthopedic Surgery, Sixth Medical Center of Chinese PLA General HospitalAbstract Background Kashin–Beck disease (KBD) is a disabling osteoarticular disease involving growth and joint cartilage. Early diagnosis can effectively prevent the progress of the disease. However, the early diagnosis of it is still very difficult. Our aim was to study the knee joint lesions of a rat KBD model using ultra-high field magnetic resonance imaging (MRI) and compare it with X-ray imaging to analyze the possible MRI manifestations of KBD, and to further explore ways to determine the pathological damage of KBD in the early stage. Methods A total of 96 Wistar rats were selected and randomly divided into 4 groups: normal diet (Group A), KBD-affected diet (Group B), normal diet+T-2 toxin (Group C), and KBD-affected diet+T-2 toxin (Group D). T-2 toxin was administered at a dose of 0.1 mg/kg/day. In the 4th week, 8th week, and 12th week, eight rats randomly selected in each group were sacrificed by cervical dislocation after undergoing X-ray and 7.0 T MRI imaging, and then knee joints were harvested, sliced, and subjected to hematoxylin-eosin (H&E) staining. Results Characteristic image changes including of continuity interruption and early closure and fusion of epiphyseal plates were observed on T1WI in rat model of KBD. The total necrosis rates in the H&E stain of group A to group D were 4.35, 52.38, 33.3, and 73.68%, respectively. The positive rate of image change under 7.0 T MRI was 0.833 VS. that under X-ray was 0.33 (P = 0.001). Conclusions MRI at 7.0 T is highly sensitive to the early pathological changes of the epiphysis, epiphyseal plate, and metaphyseal end, which can improve imaging positive rate of KBD and decrease the rate of missed diagnosis. This imaging modality can be used for research on early joint lesions and for early diagnosis of KBD.http://link.springer.com/article/10.1186/s12891-020-03559-wKashin-Beck diseaseKnee jointMagnetic resonance imagingEpiphyseal plateRat |
| spellingShingle | Yong Li Pengde Kang Zongke Zhou Fuxing Pei Qing He Dike Ruan Magnetic resonance imaging at 7.0 T for evaluation of early lesions of epiphyseal plate and epiphyseal end in a rat model of Kashin-Beck disease BMC Musculoskeletal Disorders Kashin-Beck disease Knee joint Magnetic resonance imaging Epiphyseal plate Rat |
| title | Magnetic resonance imaging at 7.0 T for evaluation of early lesions of epiphyseal plate and epiphyseal end in a rat model of Kashin-Beck disease |
| title_full | Magnetic resonance imaging at 7.0 T for evaluation of early lesions of epiphyseal plate and epiphyseal end in a rat model of Kashin-Beck disease |
| title_fullStr | Magnetic resonance imaging at 7.0 T for evaluation of early lesions of epiphyseal plate and epiphyseal end in a rat model of Kashin-Beck disease |
| title_full_unstemmed | Magnetic resonance imaging at 7.0 T for evaluation of early lesions of epiphyseal plate and epiphyseal end in a rat model of Kashin-Beck disease |
| title_short | Magnetic resonance imaging at 7.0 T for evaluation of early lesions of epiphyseal plate and epiphyseal end in a rat model of Kashin-Beck disease |
| title_sort | magnetic resonance imaging at 7 0 t for evaluation of early lesions of epiphyseal plate and epiphyseal end in a rat model of kashin beck disease |
| topic | Kashin-Beck disease Knee joint Magnetic resonance imaging Epiphyseal plate Rat |
| url | http://link.springer.com/article/10.1186/s12891-020-03559-w |
| work_keys_str_mv | AT yongli magneticresonanceimagingat70tforevaluationofearlylesionsofepiphysealplateandepiphysealendinaratmodelofkashinbeckdisease AT pengdekang magneticresonanceimagingat70tforevaluationofearlylesionsofepiphysealplateandepiphysealendinaratmodelofkashinbeckdisease AT zongkezhou magneticresonanceimagingat70tforevaluationofearlylesionsofepiphysealplateandepiphysealendinaratmodelofkashinbeckdisease AT fuxingpei magneticresonanceimagingat70tforevaluationofearlylesionsofepiphysealplateandepiphysealendinaratmodelofkashinbeckdisease AT qinghe magneticresonanceimagingat70tforevaluationofearlylesionsofepiphysealplateandepiphysealendinaratmodelofkashinbeckdisease AT dikeruan magneticresonanceimagingat70tforevaluationofearlylesionsofepiphysealplateandepiphysealendinaratmodelofkashinbeckdisease |