Blue Light Perception by Both Roots and Rhizobia Inhibits Nodule Formation in Lotus japonicus
In many legumes, roots that are exposed to light do not form nodules. Here, we report that blue light inhibits nodulation in Lotus japonicus roots inoculated with Mesorhizobium loti. Using RNA interference, we suppressed the expression of the phototropin and cryptochrome genes in L. japonicus hairy...
| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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The American Phytopathological Society
2016-10-01
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| Series: | Molecular Plant-Microbe Interactions |
| Online Access: | https://apsjournals.apsnet.org/doi/10.1094/MPMI-03-16-0048-R |
| _version_ | 1811287246426341376 |
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| author | Aya Shimomura Ayumi Naka Nobuyuki Miyazaki Sayaka Moriuchi Susumu Arima Shusei Sato Hideki Hirakawa Makoto Hayashi Maskit Maymon Ann M. Hirsch Akihiro Suzuki |
| author_facet | Aya Shimomura Ayumi Naka Nobuyuki Miyazaki Sayaka Moriuchi Susumu Arima Shusei Sato Hideki Hirakawa Makoto Hayashi Maskit Maymon Ann M. Hirsch Akihiro Suzuki |
| author_sort | Aya Shimomura |
| collection | DOAJ |
| description | In many legumes, roots that are exposed to light do not form nodules. Here, we report that blue light inhibits nodulation in Lotus japonicus roots inoculated with Mesorhizobium loti. Using RNA interference, we suppressed the expression of the phototropin and cryptochrome genes in L. japonicus hairy roots. Under blue light, plants transformed with an empty vector did not develop nodules, whereas plants exhibiting suppressed expression of cry1 and cry2 genes formed nodules. We also measured rhizobial growth to investigate whether the inhibition of nodulation could be caused by a reduced population of rhizobia in response to light. Although red light had no effect on rhizobial growth, blue light had a strong inhibitory effect. Rhizobial growth under blue light was partially restored in signature-tagged mutagenesis (STM) strains in which LOV-HK/PAS- and photolyase-related genes were disrupted. Moreover, when Ljcry1A and Ljcry2B-silenced plants were inoculated with the STM strains, nodulation was additively increased. Our data show that blue light receptors in both the host plant and the symbiont have a profound effect on nodule development. The exact mechanism by which these photomorphogenetic responses function in the symbiosis needs further study, but they are clearly involved in optimizing legume nodulation. |
| first_indexed | 2024-04-13T03:15:18Z |
| format | Article |
| id | doaj.art-77677c59029d4d6ca52bd8c7667cc451 |
| institution | Directory Open Access Journal |
| issn | 0894-0282 1943-7706 |
| language | English |
| last_indexed | 2024-04-13T03:15:18Z |
| publishDate | 2016-10-01 |
| publisher | The American Phytopathological Society |
| record_format | Article |
| series | Molecular Plant-Microbe Interactions |
| spelling | doaj.art-77677c59029d4d6ca52bd8c7667cc4512022-12-22T03:04:56ZengThe American Phytopathological SocietyMolecular Plant-Microbe Interactions0894-02821943-77062016-10-01291078679610.1094/MPMI-03-16-0048-RBlue Light Perception by Both Roots and Rhizobia Inhibits Nodule Formation in Lotus japonicusAya Shimomura0Ayumi Naka1Nobuyuki Miyazaki2Sayaka Moriuchi3Susumu Arima4Shusei Sato5Hideki Hirakawa6Makoto Hayashi7Maskit Maymon8Ann M. Hirsch9Akihiro Suzuki10United Graduate School of Agricultural Sciences, Kagoshima University, Korimoto, Kagoshima 890-0065, Japan;Department of Agro-Environmental Sciences, Faculty of Agriculture, Saga University, Honjyo-machi, Saga, Saga 840-8502, Japan;Department of Agro-Environmental Sciences, Faculty of Agriculture, Saga University, Honjyo-machi, Saga, Saga 840-8502, Japan;Department of Agro-Environmental Sciences, Faculty of Agriculture, Saga University, Honjyo-machi, Saga, Saga 840-8502, Japan;United Graduate School of Agricultural Sciences, Kagoshima University, Korimoto, Kagoshima 890-0065, Japan;Department of Environmental Life Sciences, Tohoku University, Katahira, Aoba-ku, Sendai 980-8577, Japan;Kazusa DNA Research Institute, Kazusa-kamatari, Kisarazu, Chiba 292-0818, Japan;RIKEN Plant Science Center, Yokohama, Kanagawa 230-0045, Japan;Department of Molecular, Cell and Developmental Biology, University of California-Los Angeles, Los Angeles, CA 90095-1606, U.S.A.; andDepartment of Molecular, Cell and Developmental Biology, University of California-Los Angeles, Los Angeles, CA 90095-1606, U.S.A.; andUnited Graduate School of Agricultural Sciences, Kagoshima University, Korimoto, Kagoshima 890-0065, Japan;In many legumes, roots that are exposed to light do not form nodules. Here, we report that blue light inhibits nodulation in Lotus japonicus roots inoculated with Mesorhizobium loti. Using RNA interference, we suppressed the expression of the phototropin and cryptochrome genes in L. japonicus hairy roots. Under blue light, plants transformed with an empty vector did not develop nodules, whereas plants exhibiting suppressed expression of cry1 and cry2 genes formed nodules. We also measured rhizobial growth to investigate whether the inhibition of nodulation could be caused by a reduced population of rhizobia in response to light. Although red light had no effect on rhizobial growth, blue light had a strong inhibitory effect. Rhizobial growth under blue light was partially restored in signature-tagged mutagenesis (STM) strains in which LOV-HK/PAS- and photolyase-related genes were disrupted. Moreover, when Ljcry1A and Ljcry2B-silenced plants were inoculated with the STM strains, nodulation was additively increased. Our data show that blue light receptors in both the host plant and the symbiont have a profound effect on nodule development. The exact mechanism by which these photomorphogenetic responses function in the symbiosis needs further study, but they are clearly involved in optimizing legume nodulation.https://apsjournals.apsnet.org/doi/10.1094/MPMI-03-16-0048-R |
| spellingShingle | Aya Shimomura Ayumi Naka Nobuyuki Miyazaki Sayaka Moriuchi Susumu Arima Shusei Sato Hideki Hirakawa Makoto Hayashi Maskit Maymon Ann M. Hirsch Akihiro Suzuki Blue Light Perception by Both Roots and Rhizobia Inhibits Nodule Formation in Lotus japonicus Molecular Plant-Microbe Interactions |
| title | Blue Light Perception by Both Roots and Rhizobia Inhibits Nodule Formation in Lotus japonicus |
| title_full | Blue Light Perception by Both Roots and Rhizobia Inhibits Nodule Formation in Lotus japonicus |
| title_fullStr | Blue Light Perception by Both Roots and Rhizobia Inhibits Nodule Formation in Lotus japonicus |
| title_full_unstemmed | Blue Light Perception by Both Roots and Rhizobia Inhibits Nodule Formation in Lotus japonicus |
| title_short | Blue Light Perception by Both Roots and Rhizobia Inhibits Nodule Formation in Lotus japonicus |
| title_sort | blue light perception by both roots and rhizobia inhibits nodule formation in lotus japonicus |
| url | https://apsjournals.apsnet.org/doi/10.1094/MPMI-03-16-0048-R |
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