Inactivation of SARS-CoV-2 on Surfaces by Cold-Plasma-Generated Reactive Species
A Cold Atmospheric Plasma (CAP) apparatus was designed and developed for SARS-CoV-2 killing as evaluated by pseudotyped viral infectivity assays. The reactive species generated by the plasma system was fully characterized by using Optical Emission Spectroscopy (OES) measurement under given condition...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-02-01
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| Series: | Bioengineering |
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| Online Access: | https://www.mdpi.com/2306-5354/10/3/280 |
| _version_ | 1797613416642248704 |
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| author | Som V. Thomas Krista Dienger-Stambaugh Michael Jordan Yuxin Wang Jason Hammonds Paul Spearman Donglu Shi |
| author_facet | Som V. Thomas Krista Dienger-Stambaugh Michael Jordan Yuxin Wang Jason Hammonds Paul Spearman Donglu Shi |
| author_sort | Som V. Thomas |
| collection | DOAJ |
| description | A Cold Atmospheric Plasma (CAP) apparatus was designed and developed for SARS-CoV-2 killing as evaluated by pseudotyped viral infectivity assays. The reactive species generated by the plasma system was fully characterized by using Optical Emission Spectroscopy (OES) measurement under given conditions such as plasma power, flow rate, and treatment time. A variety of reactive oxygen species (ROS) and reactive nitrogen species (RNS) were identified from plasma plume with energies of 15–72 eV in the frequency range between 500–1000 nm. Systematic virus killing experiments were carried out, and the efficacy of CAP treatment in reducing SARS-CoV-2 viral infectivity was significant following treatment for 8 s, with further enhancement of killing upon longer exposures of 15–120 s. We correlated killing efficacy with the reactive species in terms of type, intensity, energy, and frequency. These experimental results demonstrate effective cold plasma virus killing via ROS and RNS under ambient conditions. |
| first_indexed | 2024-03-11T06:55:31Z |
| format | Article |
| id | doaj.art-776ab0052cc34625b6d90c867af983b1 |
| institution | Directory Open Access Journal |
| issn | 2306-5354 |
| language | English |
| last_indexed | 2024-03-11T06:55:31Z |
| publishDate | 2023-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Bioengineering |
| spelling | doaj.art-776ab0052cc34625b6d90c867af983b12023-11-17T09:39:03ZengMDPI AGBioengineering2306-53542023-02-0110328010.3390/bioengineering10030280Inactivation of SARS-CoV-2 on Surfaces by Cold-Plasma-Generated Reactive SpeciesSom V. Thomas0Krista Dienger-Stambaugh1Michael Jordan2Yuxin Wang3Jason Hammonds4Paul Spearman5Donglu Shi6Department of Biomedical Engineering, College of Engineering and Applied Science, University of Cincinnati, Cincinnati, OH 45221, USADivision of Infectious Diseases, Cincinnati Children’s Hospital Medical Center and University of Cincinnati, Cincinnati, OH 45229, USADivision of Infectious Diseases, Cincinnati Children’s Hospital Medical Center and University of Cincinnati, Cincinnati, OH 45229, USADepartment of Biomedical Engineering, College of Engineering and Applied Science, University of Cincinnati, Cincinnati, OH 45221, USADivision of Infectious Diseases, Cincinnati Children’s Hospital Medical Center and University of Cincinnati, Cincinnati, OH 45229, USADivision of Infectious Diseases, Cincinnati Children’s Hospital Medical Center and University of Cincinnati, Cincinnati, OH 45229, USADepartment of Biomedical Engineering, College of Engineering and Applied Science, University of Cincinnati, Cincinnati, OH 45221, USAA Cold Atmospheric Plasma (CAP) apparatus was designed and developed for SARS-CoV-2 killing as evaluated by pseudotyped viral infectivity assays. The reactive species generated by the plasma system was fully characterized by using Optical Emission Spectroscopy (OES) measurement under given conditions such as plasma power, flow rate, and treatment time. A variety of reactive oxygen species (ROS) and reactive nitrogen species (RNS) were identified from plasma plume with energies of 15–72 eV in the frequency range between 500–1000 nm. Systematic virus killing experiments were carried out, and the efficacy of CAP treatment in reducing SARS-CoV-2 viral infectivity was significant following treatment for 8 s, with further enhancement of killing upon longer exposures of 15–120 s. We correlated killing efficacy with the reactive species in terms of type, intensity, energy, and frequency. These experimental results demonstrate effective cold plasma virus killing via ROS and RNS under ambient conditions.https://www.mdpi.com/2306-5354/10/3/280cold atmospheric plasmaCOVID-19virus inactivationoxygen reactive species |
| spellingShingle | Som V. Thomas Krista Dienger-Stambaugh Michael Jordan Yuxin Wang Jason Hammonds Paul Spearman Donglu Shi Inactivation of SARS-CoV-2 on Surfaces by Cold-Plasma-Generated Reactive Species Bioengineering cold atmospheric plasma COVID-19 virus inactivation oxygen reactive species |
| title | Inactivation of SARS-CoV-2 on Surfaces by Cold-Plasma-Generated Reactive Species |
| title_full | Inactivation of SARS-CoV-2 on Surfaces by Cold-Plasma-Generated Reactive Species |
| title_fullStr | Inactivation of SARS-CoV-2 on Surfaces by Cold-Plasma-Generated Reactive Species |
| title_full_unstemmed | Inactivation of SARS-CoV-2 on Surfaces by Cold-Plasma-Generated Reactive Species |
| title_short | Inactivation of SARS-CoV-2 on Surfaces by Cold-Plasma-Generated Reactive Species |
| title_sort | inactivation of sars cov 2 on surfaces by cold plasma generated reactive species |
| topic | cold atmospheric plasma COVID-19 virus inactivation oxygen reactive species |
| url | https://www.mdpi.com/2306-5354/10/3/280 |
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