Field Observations and Genetic Characterization of Sheep-Associated Malignant Catarrhal Fever in Egypt, 2018
Ovine gammaherpesvirus-2 (OvHV-2) causes a lethal disease in cattle and some wild ruminants called malignant catarrhal fever (MCF), which affects the epithelial and lymphoid tissues of the respiratory and digestive tracts and has an important impact on the livestock industry. In this study, MCF was...
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MDPI AG
2020-12-01
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| Series: | Veterinary Sciences |
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| Online Access: | https://www.mdpi.com/2306-7381/7/4/201 |
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| author | Sahar Abd El Rahman Ahmed Ateya Mohamed El-Beskawy Kerstin Wernike Bernd Hoffmann Michael Eschbaumer |
| author_facet | Sahar Abd El Rahman Ahmed Ateya Mohamed El-Beskawy Kerstin Wernike Bernd Hoffmann Michael Eschbaumer |
| author_sort | Sahar Abd El Rahman |
| collection | DOAJ |
| description | Ovine gammaherpesvirus-2 (OvHV-2) causes a lethal disease in cattle and some wild ruminants called malignant catarrhal fever (MCF), which affects the epithelial and lymphoid tissues of the respiratory and digestive tracts and has an important impact on the livestock industry. In this study, MCF was diagnosed in 18 of 427 cattle from different sites in Egypt by its typical clinical signs, found in all 18 animals: corneal opacity, fever, erosions in the buccal cavity, lymphadenitis, and purulent nasal discharge. All affected cattle had been reared in contact with clinically inconspicuous sheep. Of the 18 clinically ill cattle, 13 succumbed to the disease, resulting in estimated morbidity and case fatality rates of 4.2% and 72.2%, respectively. Five samples collected from the affected cattle were positive for OvHV-2 by real-time PCR and were used for sequencing of an 832-bp fragment of the ORF27/gp48 gene. The ORF27 nucleotide sequence of all Egyptian samples was identical, but distinct from viruses found in other parts of Africa and the Mediterranean. |
| first_indexed | 2024-03-10T14:08:53Z |
| format | Article |
| id | doaj.art-776abda34df04167a6b0dd0db52a6c40 |
| institution | Directory Open Access Journal |
| issn | 2306-7381 |
| language | English |
| last_indexed | 2024-03-10T14:08:53Z |
| publishDate | 2020-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Veterinary Sciences |
| spelling | doaj.art-776abda34df04167a6b0dd0db52a6c402023-11-21T00:22:19ZengMDPI AGVeterinary Sciences2306-73812020-12-017420110.3390/vetsci7040201Field Observations and Genetic Characterization of Sheep-Associated Malignant Catarrhal Fever in Egypt, 2018Sahar Abd El Rahman0Ahmed Ateya1Mohamed El-Beskawy2Kerstin Wernike3Bernd Hoffmann4Michael Eschbaumer5Department of Virology, Faculty of Veterinary Medicine, Mansoura University, Mansoura 35516, EgyptDepartment of Animal Husbandry and Development of Animal Wealth, Faculty of Veterinary Medicine, Mansoura University, Mansoura 35516, EgyptDepartment of Animal Medicine, Faculty of Veterinary Medicine, Matrouh University, Matrouh 51744, EgyptInstitute of Diagnostic Virology, Friedrich-Loeffler-Institut, 17493 Greifswald-Insel Riems, GermanyInstitute of Diagnostic Virology, Friedrich-Loeffler-Institut, 17493 Greifswald-Insel Riems, GermanyInstitute of Diagnostic Virology, Friedrich-Loeffler-Institut, 17493 Greifswald-Insel Riems, GermanyOvine gammaherpesvirus-2 (OvHV-2) causes a lethal disease in cattle and some wild ruminants called malignant catarrhal fever (MCF), which affects the epithelial and lymphoid tissues of the respiratory and digestive tracts and has an important impact on the livestock industry. In this study, MCF was diagnosed in 18 of 427 cattle from different sites in Egypt by its typical clinical signs, found in all 18 animals: corneal opacity, fever, erosions in the buccal cavity, lymphadenitis, and purulent nasal discharge. All affected cattle had been reared in contact with clinically inconspicuous sheep. Of the 18 clinically ill cattle, 13 succumbed to the disease, resulting in estimated morbidity and case fatality rates of 4.2% and 72.2%, respectively. Five samples collected from the affected cattle were positive for OvHV-2 by real-time PCR and were used for sequencing of an 832-bp fragment of the ORF27/gp48 gene. The ORF27 nucleotide sequence of all Egyptian samples was identical, but distinct from viruses found in other parts of Africa and the Mediterranean.https://www.mdpi.com/2306-7381/7/4/201sheep-associated malignant catarrhal feverovine gammaherpesvirus 2ORF27gp48Egypt |
| spellingShingle | Sahar Abd El Rahman Ahmed Ateya Mohamed El-Beskawy Kerstin Wernike Bernd Hoffmann Michael Eschbaumer Field Observations and Genetic Characterization of Sheep-Associated Malignant Catarrhal Fever in Egypt, 2018 Veterinary Sciences sheep-associated malignant catarrhal fever ovine gammaherpesvirus 2 ORF27 gp48 Egypt |
| title | Field Observations and Genetic Characterization of Sheep-Associated Malignant Catarrhal Fever in Egypt, 2018 |
| title_full | Field Observations and Genetic Characterization of Sheep-Associated Malignant Catarrhal Fever in Egypt, 2018 |
| title_fullStr | Field Observations and Genetic Characterization of Sheep-Associated Malignant Catarrhal Fever in Egypt, 2018 |
| title_full_unstemmed | Field Observations and Genetic Characterization of Sheep-Associated Malignant Catarrhal Fever in Egypt, 2018 |
| title_short | Field Observations and Genetic Characterization of Sheep-Associated Malignant Catarrhal Fever in Egypt, 2018 |
| title_sort | field observations and genetic characterization of sheep associated malignant catarrhal fever in egypt 2018 |
| topic | sheep-associated malignant catarrhal fever ovine gammaherpesvirus 2 ORF27 gp48 Egypt |
| url | https://www.mdpi.com/2306-7381/7/4/201 |
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