Melatonin treatment alleviates chilling injury in mango fruit ‘Keitt’ by modulating proline metabolism under chilling stress
Mangoes often suffer from low temperature-induced chilling injury (CI) during postharvest cold storage. Therefore, advanced techniques are crucial and in high demand to solve the chilling stress of mango fruit for a higher value. This study addresses chilling stress modulation by investigating the e...
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| Format: | Article |
| Language: | English |
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Elsevier
2023-03-01
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| Series: | Journal of Integrative Agriculture |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2095311923000205 |
| _version_ | 1797869845754150912 |
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| author | Mariama KEBBEH Jing-xian DONG Chen HUAN Yan LIU Xiao-lin ZHENG |
| author_facet | Mariama KEBBEH Jing-xian DONG Chen HUAN Yan LIU Xiao-lin ZHENG |
| author_sort | Mariama KEBBEH |
| collection | DOAJ |
| description | Mangoes often suffer from low temperature-induced chilling injury (CI) during postharvest cold storage. Therefore, advanced techniques are crucial and in high demand to solve the chilling stress of mango fruit for a higher value. This study addresses chilling stress modulation by investigating the effects of melatonin treatment on CI, proline metabolism, and related gene expressions of ‘Keitt’ mango during cold storage after dipped in 0 (control), 0.1 (MT1), and 0.2 mmol L−1 (MT2) melatonin solution for 30 min. The results revealed that melatonin treatment in MT1 significantly reduced CI development and increased proline content in mango fruit during cold storage compared to the control. These changes were along with increases in the activity of critical enzymes as well as the expression of encoding genes involved in proline biosynthesis, such as pyrroline-5-carboxylate synthetase (P5CS), pyrroline-5-carboxylate reductase (P5CR), ornithine D-aminotransferase (OAT), P5CS2, P5CR2, and OAT3. Additionally, proline dehydrogenase (PDH) activity and the expression of the PDH3 gene associated with proline dehydrogenation were lower in MT1-treated mangoes than the controlled group. Thus, melatonin treatment has regulated proline metabolism resulting in the accumulation of proline, subsequently contributing to enhancing the chilling tolerance of ‘Keitt’ mango fruit. |
| first_indexed | 2024-04-10T00:17:57Z |
| format | Article |
| id | doaj.art-776bcc6575d94cffa5650272fe485237 |
| institution | Directory Open Access Journal |
| issn | 2095-3119 |
| language | English |
| last_indexed | 2024-04-10T00:17:57Z |
| publishDate | 2023-03-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Integrative Agriculture |
| spelling | doaj.art-776bcc6575d94cffa5650272fe4852372023-03-16T05:03:45ZengElsevierJournal of Integrative Agriculture2095-31192023-03-01223935944Melatonin treatment alleviates chilling injury in mango fruit ‘Keitt’ by modulating proline metabolism under chilling stressMariama KEBBEH0Jing-xian DONG1Chen HUAN2Yan LIU3Xiao-lin ZHENG4College of Food Science and Biotechnology, Zhejiang Gongshang University, Hangzhou 310018, P.R.ChinaCollege of Food Science and Biotechnology, Zhejiang Gongshang University, Hangzhou 310018, P.R.ChinaCollege of Food Science and Biotechnology, Zhejiang Gongshang University, Hangzhou 310018, P.R.ChinaCollege of Food Science and Biotechnology, Zhejiang Gongshang University, Hangzhou 310018, P.R.ChinaCorrespondence ZHENG Xiao-lin; College of Food Science and Biotechnology, Zhejiang Gongshang University, Hangzhou 310018, P.R.ChinaMangoes often suffer from low temperature-induced chilling injury (CI) during postharvest cold storage. Therefore, advanced techniques are crucial and in high demand to solve the chilling stress of mango fruit for a higher value. This study addresses chilling stress modulation by investigating the effects of melatonin treatment on CI, proline metabolism, and related gene expressions of ‘Keitt’ mango during cold storage after dipped in 0 (control), 0.1 (MT1), and 0.2 mmol L−1 (MT2) melatonin solution for 30 min. The results revealed that melatonin treatment in MT1 significantly reduced CI development and increased proline content in mango fruit during cold storage compared to the control. These changes were along with increases in the activity of critical enzymes as well as the expression of encoding genes involved in proline biosynthesis, such as pyrroline-5-carboxylate synthetase (P5CS), pyrroline-5-carboxylate reductase (P5CR), ornithine D-aminotransferase (OAT), P5CS2, P5CR2, and OAT3. Additionally, proline dehydrogenase (PDH) activity and the expression of the PDH3 gene associated with proline dehydrogenation were lower in MT1-treated mangoes than the controlled group. Thus, melatonin treatment has regulated proline metabolism resulting in the accumulation of proline, subsequently contributing to enhancing the chilling tolerance of ‘Keitt’ mango fruit.http://www.sciencedirect.com/science/article/pii/S2095311923000205chilling injury (CI)cold storagemango fruitmelatoninproline metabolism |
| spellingShingle | Mariama KEBBEH Jing-xian DONG Chen HUAN Yan LIU Xiao-lin ZHENG Melatonin treatment alleviates chilling injury in mango fruit ‘Keitt’ by modulating proline metabolism under chilling stress Journal of Integrative Agriculture chilling injury (CI) cold storage mango fruit melatonin proline metabolism |
| title | Melatonin treatment alleviates chilling injury in mango fruit ‘Keitt’ by modulating proline metabolism under chilling stress |
| title_full | Melatonin treatment alleviates chilling injury in mango fruit ‘Keitt’ by modulating proline metabolism under chilling stress |
| title_fullStr | Melatonin treatment alleviates chilling injury in mango fruit ‘Keitt’ by modulating proline metabolism under chilling stress |
| title_full_unstemmed | Melatonin treatment alleviates chilling injury in mango fruit ‘Keitt’ by modulating proline metabolism under chilling stress |
| title_short | Melatonin treatment alleviates chilling injury in mango fruit ‘Keitt’ by modulating proline metabolism under chilling stress |
| title_sort | melatonin treatment alleviates chilling injury in mango fruit keitt by modulating proline metabolism under chilling stress |
| topic | chilling injury (CI) cold storage mango fruit melatonin proline metabolism |
| url | http://www.sciencedirect.com/science/article/pii/S2095311923000205 |
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