Use of a highly sensitive lung cancer compact panel to detect KRAS G12D in the wash fluid from a lung tumor: A case report
Abstract Here, we report a case of a pulmonary invasive mucinous adenocarcinoma harboring KRAS G12D, diagnosed from tumor samples containing a very small amount of tumor cells using next‐generation sequencing (NGS) and the recently developed Lung Cancer Compact Panel. A 79‐year‐old woman without any...
| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Wiley
2022-06-01
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| Series: | Thoracic Cancer |
| Subjects: | |
| Online Access: | https://doi.org/10.1111/1759-7714.14439 |
| _version_ | 1828807191201579008 |
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| author | Daisuke Minami Nagio Takigawa Yasuhiro Nakajima Nobuaki Miyahara Yasuyuki Mizumori Mitsuhiro Ueda Seiji Nakamura Fumihiko Suzuki Yoshiharu Sato Kei Morikawa Arihiko Kanehiro |
| author_facet | Daisuke Minami Nagio Takigawa Yasuhiro Nakajima Nobuaki Miyahara Yasuyuki Mizumori Mitsuhiro Ueda Seiji Nakamura Fumihiko Suzuki Yoshiharu Sato Kei Morikawa Arihiko Kanehiro |
| author_sort | Daisuke Minami |
| collection | DOAJ |
| description | Abstract Here, we report a case of a pulmonary invasive mucinous adenocarcinoma harboring KRAS G12D, diagnosed from tumor samples containing a very small amount of tumor cells using next‐generation sequencing (NGS) and the recently developed Lung Cancer Compact Panel. A 79‐year‐old woman without any respiratory symptoms underwent chest computed tomography, which revealed a tumor in the left lower lobe. During endobronchial ultrasound (EBUS)‐guided transbronchial biopsy (TBB) using a guide sheath (GS), a sufficient specimen for pathological diagnosis could not be obtained because the patient had a severe cough and pulmonary bullae located adjacent to the tumor. In the absence of EBUS transbronchial biopsy findings using a guide sheath, brush cytology was used to categorize the tumor as class II (Papanicolaou classification). However, the wash fluid from the cytological examination contained enough cells to obtain sufficient nucleic acid for use in sequencing analysis. The latter revealed KRAS G12D expression. Although the patient underwent surgery without pathological evidence, the evaluation of the surgical specimen confirmed a diagnosis of pulmonary invasive mucinous adenocarcinoma. Use of the Lung Cancer Compact Panel enabled the detection of KRAS G12D in the wash fluid of a brush cytology sample and thus a diagnosis of pulmonary invasive mucinous adenocarcinoma. |
| first_indexed | 2024-12-12T08:21:05Z |
| format | Article |
| id | doaj.art-776c21d1b0314ce1b5900e00d90e462c |
| institution | Directory Open Access Journal |
| issn | 1759-7706 1759-7714 |
| language | English |
| last_indexed | 2024-12-12T08:21:05Z |
| publishDate | 2022-06-01 |
| publisher | Wiley |
| record_format | Article |
| series | Thoracic Cancer |
| spelling | doaj.art-776c21d1b0314ce1b5900e00d90e462c2022-12-22T00:31:23ZengWileyThoracic Cancer1759-77061759-77142022-06-0113111735173810.1111/1759-7714.14439Use of a highly sensitive lung cancer compact panel to detect KRAS G12D in the wash fluid from a lung tumor: A case reportDaisuke Minami0Nagio Takigawa1Yasuhiro Nakajima2Nobuaki Miyahara3Yasuyuki Mizumori4Mitsuhiro Ueda5Seiji Nakamura6Fumihiko Suzuki7Yoshiharu Sato8Kei Morikawa9Arihiko Kanehiro10Department of Respiratory Medicine Himeji Saint Mary's Hospital Himeji JapanDepartment of Internal Medicine Himeji Saint Mary's Hospital Himeji JapanDepartment of Respiratory Medicine Himeji Saint Mary's Hospital Himeji JapanDepartment of Internal Medicine Himeji Saint Mary's Hospital Himeji JapanDepartment of Respiratory Medicine National Hospital Organization Himeji Medical Center Himeji JapanDepartment of Thoracic Surgery National Hospital Organization Himeji Medical Center JapanDNA Chip Research Inc JapanDNA Chip Research Inc JapanDNA Chip Research Inc JapanDivision of Respiratory Medicine, Department of Internal Medicine St. Marianna University School of Medicine Kawasaki JapanDepartment of Internal Medicine Himeji Saint Mary's Hospital Himeji JapanAbstract Here, we report a case of a pulmonary invasive mucinous adenocarcinoma harboring KRAS G12D, diagnosed from tumor samples containing a very small amount of tumor cells using next‐generation sequencing (NGS) and the recently developed Lung Cancer Compact Panel. A 79‐year‐old woman without any respiratory symptoms underwent chest computed tomography, which revealed a tumor in the left lower lobe. During endobronchial ultrasound (EBUS)‐guided transbronchial biopsy (TBB) using a guide sheath (GS), a sufficient specimen for pathological diagnosis could not be obtained because the patient had a severe cough and pulmonary bullae located adjacent to the tumor. In the absence of EBUS transbronchial biopsy findings using a guide sheath, brush cytology was used to categorize the tumor as class II (Papanicolaou classification). However, the wash fluid from the cytological examination contained enough cells to obtain sufficient nucleic acid for use in sequencing analysis. The latter revealed KRAS G12D expression. Although the patient underwent surgery without pathological evidence, the evaluation of the surgical specimen confirmed a diagnosis of pulmonary invasive mucinous adenocarcinoma. Use of the Lung Cancer Compact Panel enabled the detection of KRAS G12D in the wash fluid of a brush cytology sample and thus a diagnosis of pulmonary invasive mucinous adenocarcinoma.https://doi.org/10.1111/1759-7714.14439KRAS G12Dlung cancer compact panelpulmonary invasive mucinous adenocarcinoma |
| spellingShingle | Daisuke Minami Nagio Takigawa Yasuhiro Nakajima Nobuaki Miyahara Yasuyuki Mizumori Mitsuhiro Ueda Seiji Nakamura Fumihiko Suzuki Yoshiharu Sato Kei Morikawa Arihiko Kanehiro Use of a highly sensitive lung cancer compact panel to detect KRAS G12D in the wash fluid from a lung tumor: A case report Thoracic Cancer KRAS G12D lung cancer compact panel pulmonary invasive mucinous adenocarcinoma |
| title | Use of a highly sensitive lung cancer compact panel to detect KRAS G12D in the wash fluid from a lung tumor: A case report |
| title_full | Use of a highly sensitive lung cancer compact panel to detect KRAS G12D in the wash fluid from a lung tumor: A case report |
| title_fullStr | Use of a highly sensitive lung cancer compact panel to detect KRAS G12D in the wash fluid from a lung tumor: A case report |
| title_full_unstemmed | Use of a highly sensitive lung cancer compact panel to detect KRAS G12D in the wash fluid from a lung tumor: A case report |
| title_short | Use of a highly sensitive lung cancer compact panel to detect KRAS G12D in the wash fluid from a lung tumor: A case report |
| title_sort | use of a highly sensitive lung cancer compact panel to detect kras g12d in the wash fluid from a lung tumor a case report |
| topic | KRAS G12D lung cancer compact panel pulmonary invasive mucinous adenocarcinoma |
| url | https://doi.org/10.1111/1759-7714.14439 |
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