Ammonia oxidizing bacteria community dynamics in a pilot-scale wastewater treatment plant.
BACKGROUND: Chemoautotrophic ammonia oxidizing bacteria (AOB) have the metabolic ability to oxidize ammonia to nitrite aerobically. This metabolic feature has been widely used, in combination with denitrification, to remove nitrogen from wastewater in wastewater treatment plants (WWTPs). However, th...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2012-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC3338686?pdf=render |
| _version_ | 1818695989920268288 |
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| author | Xiaohui Wang Xianghua Wen Yu Xia Ma Hu Fang Zhao Kun Ding |
| author_facet | Xiaohui Wang Xianghua Wen Yu Xia Ma Hu Fang Zhao Kun Ding |
| author_sort | Xiaohui Wang |
| collection | DOAJ |
| description | BACKGROUND: Chemoautotrophic ammonia oxidizing bacteria (AOB) have the metabolic ability to oxidize ammonia to nitrite aerobically. This metabolic feature has been widely used, in combination with denitrification, to remove nitrogen from wastewater in wastewater treatment plants (WWTPs). However, the relative influence of specific deterministic environmental factors to AOB community dynamics in WWTP is uncertain. The ecological principles underlying AOB community dynamics and nitrification stability and how they are related are also poorly understood. METHODOLOGY/PRINCIPAL FINDINGS: The community dynamics of ammonia oxidizing bacteria (AOB) in a pilot-scale WWTP were monitored over a one-year period by Terminal Restriction Fragment Length Polymorphism (T-RFLP). During the study period, the effluent ammonia concentrations were almost below 2 mg/L, except for the first 60 days, indicting stable nitrification. T-RFLP results showed that, during the test period with stable nitrification, the AOB community structures were not stable, and the average change rate (every 15 days) of AOB community structures was 10% ± 8%. The correlations between T-RFLP profiles and 10 operational and environmental parameters were tested by Canonical Correlation Analysis (CCA) and Mantel test. The results indicated that the dynamics of AOB community correlated most strongly with Dissolved Oxygen (DO), effluent ammonia, effluent Biochemical Oxygen Demand (BOD) and temperature. CONCLUSIONS/SIGNIFICANCE: This study suggests that nitrification stability is not necessarily accompanied by a stable AOB community, and provides insight into parameters controlling the AOB community dynamics within bioreactors with stable nitrification. |
| first_indexed | 2024-12-17T13:54:14Z |
| format | Article |
| id | doaj.art-776c3e784afa4e7d816f88474f0c87d5 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-17T13:54:14Z |
| publishDate | 2012-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-776c3e784afa4e7d816f88474f0c87d52022-12-21T21:45:58ZengPublic Library of Science (PLoS)PLoS ONE1932-62032012-01-0174e3627210.1371/journal.pone.0036272Ammonia oxidizing bacteria community dynamics in a pilot-scale wastewater treatment plant.Xiaohui WangXianghua WenYu XiaMa HuFang ZhaoKun DingBACKGROUND: Chemoautotrophic ammonia oxidizing bacteria (AOB) have the metabolic ability to oxidize ammonia to nitrite aerobically. This metabolic feature has been widely used, in combination with denitrification, to remove nitrogen from wastewater in wastewater treatment plants (WWTPs). However, the relative influence of specific deterministic environmental factors to AOB community dynamics in WWTP is uncertain. The ecological principles underlying AOB community dynamics and nitrification stability and how they are related are also poorly understood. METHODOLOGY/PRINCIPAL FINDINGS: The community dynamics of ammonia oxidizing bacteria (AOB) in a pilot-scale WWTP were monitored over a one-year period by Terminal Restriction Fragment Length Polymorphism (T-RFLP). During the study period, the effluent ammonia concentrations were almost below 2 mg/L, except for the first 60 days, indicting stable nitrification. T-RFLP results showed that, during the test period with stable nitrification, the AOB community structures were not stable, and the average change rate (every 15 days) of AOB community structures was 10% ± 8%. The correlations between T-RFLP profiles and 10 operational and environmental parameters were tested by Canonical Correlation Analysis (CCA) and Mantel test. The results indicated that the dynamics of AOB community correlated most strongly with Dissolved Oxygen (DO), effluent ammonia, effluent Biochemical Oxygen Demand (BOD) and temperature. CONCLUSIONS/SIGNIFICANCE: This study suggests that nitrification stability is not necessarily accompanied by a stable AOB community, and provides insight into parameters controlling the AOB community dynamics within bioreactors with stable nitrification.http://europepmc.org/articles/PMC3338686?pdf=render |
| spellingShingle | Xiaohui Wang Xianghua Wen Yu Xia Ma Hu Fang Zhao Kun Ding Ammonia oxidizing bacteria community dynamics in a pilot-scale wastewater treatment plant. PLoS ONE |
| title | Ammonia oxidizing bacteria community dynamics in a pilot-scale wastewater treatment plant. |
| title_full | Ammonia oxidizing bacteria community dynamics in a pilot-scale wastewater treatment plant. |
| title_fullStr | Ammonia oxidizing bacteria community dynamics in a pilot-scale wastewater treatment plant. |
| title_full_unstemmed | Ammonia oxidizing bacteria community dynamics in a pilot-scale wastewater treatment plant. |
| title_short | Ammonia oxidizing bacteria community dynamics in a pilot-scale wastewater treatment plant. |
| title_sort | ammonia oxidizing bacteria community dynamics in a pilot scale wastewater treatment plant |
| url | http://europepmc.org/articles/PMC3338686?pdf=render |
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