Differential Metabolic and Transcriptional Responses of Gilthead Seabream (<i>Sparus aurata</i>) Administered with Cortisol or Cortisol-BSA
Cortisol is the main glucocorticoid hormone promoting compensatory metabolic responses of stress in teleosts. This hormone acts through genomic and membrane-initiated actions to exert its functions inside the cell. Experimental approaches, using exogenous cortisol administration, confirm the role of...
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MDPI AG
2021-11-01
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| Online Access: | https://www.mdpi.com/2076-2615/11/11/3310 |
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| author | Jorge Aedo Daniela Aravena-Canales Ignacio Ruiz-Jarabo Ricardo Oyarzún Alfredo Molina Gonzalo Martínez-Rodríguez Juan Antonio Valdés Juan Miguel Mancera |
| author_facet | Jorge Aedo Daniela Aravena-Canales Ignacio Ruiz-Jarabo Ricardo Oyarzún Alfredo Molina Gonzalo Martínez-Rodríguez Juan Antonio Valdés Juan Miguel Mancera |
| author_sort | Jorge Aedo |
| collection | DOAJ |
| description | Cortisol is the main glucocorticoid hormone promoting compensatory metabolic responses of stress in teleosts. This hormone acts through genomic and membrane-initiated actions to exert its functions inside the cell. Experimental approaches, using exogenous cortisol administration, confirm the role of this hormone during short (minutes to hours)- and long-term (days to weeks) responses to stress. The role of membrane-initiated cortisol signaling during long-term responses has been recently explored. In this study, <i>Sparus aurata</i> were intraperitoneally injected with coconut oil alone or coconut oil containing cortisol, cortisol-BSA, or BSA. After 3 days of treatment, plasma, liver, and skeletal muscle were extracted. Plasma cortisol, as well as metabolic indicators in the plasma and tissues collected, and metabolism-related gene expression, were measured. Our results showed that artificially increased plasma cortisol levels in <i>S. aurata</i> enhanced plasma glucose and triacylglycerols values as well as hepatic substrate energy mobilization. Additionally, cortisol stimulated hepatic carbohydrates metabolism, as seen by the increased expression of metabolism-related genes. All of these responses, observed in cortisol-administered fish, were not detected by replicating the same protocol and instead using cortisol-BSA, which exclusively induces membrane-initiated effects. Therefore, we suggest that after three days of cortisol administration, only genomic actions are involved in the metabolic responses in <i>S. aurata</i>. |
| first_indexed | 2024-03-10T05:46:12Z |
| format | Article |
| id | doaj.art-776cb598aebd49808d62eee471476c99 |
| institution | Directory Open Access Journal |
| issn | 2076-2615 |
| language | English |
| last_indexed | 2024-03-10T05:46:12Z |
| publishDate | 2021-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Animals |
| spelling | doaj.art-776cb598aebd49808d62eee471476c992023-11-22T22:08:42ZengMDPI AGAnimals2076-26152021-11-011111331010.3390/ani11113310Differential Metabolic and Transcriptional Responses of Gilthead Seabream (<i>Sparus aurata</i>) Administered with Cortisol or Cortisol-BSAJorge Aedo0Daniela Aravena-Canales1Ignacio Ruiz-Jarabo2Ricardo Oyarzún3Alfredo Molina4Gonzalo Martínez-Rodríguez5Juan Antonio Valdés6Juan Miguel Mancera7Department of Biological Sciences, Faculty of Life Sciences, Andres Bello University, Santiago 8320000, ChileDepartment of Biological Sciences, Faculty of Life Sciences, Andres Bello University, Santiago 8320000, ChileDepartment of Biology, Faculty of Marine and Environmental Sciences, Instituto Universitario de Investigación Marina (INMAR), Campus de Excelencia Internacional del Mar (CEI-MAR), University of Cádiz, 11519 Puerto Real, SpainInstitute of Marine and Limnological Sciences, Faculty of Sciences, University Austral of Chile, Valdivia 5110652, ChileDepartment of Biological Sciences, Faculty of Life Sciences, Andres Bello University, Santiago 8320000, ChileDepartment of Marine Biology and Aquaculture, Instituto de Ciencias Marinas de Andalucía (ICMAN-CSIC), 11519 Puerto Real, SpainDepartment of Biological Sciences, Faculty of Life Sciences, Andres Bello University, Santiago 8320000, ChileDepartment of Biology, Faculty of Marine and Environmental Sciences, Instituto Universitario de Investigación Marina (INMAR), Campus de Excelencia Internacional del Mar (CEI-MAR), University of Cádiz, 11519 Puerto Real, SpainCortisol is the main glucocorticoid hormone promoting compensatory metabolic responses of stress in teleosts. This hormone acts through genomic and membrane-initiated actions to exert its functions inside the cell. Experimental approaches, using exogenous cortisol administration, confirm the role of this hormone during short (minutes to hours)- and long-term (days to weeks) responses to stress. The role of membrane-initiated cortisol signaling during long-term responses has been recently explored. In this study, <i>Sparus aurata</i> were intraperitoneally injected with coconut oil alone or coconut oil containing cortisol, cortisol-BSA, or BSA. After 3 days of treatment, plasma, liver, and skeletal muscle were extracted. Plasma cortisol, as well as metabolic indicators in the plasma and tissues collected, and metabolism-related gene expression, were measured. Our results showed that artificially increased plasma cortisol levels in <i>S. aurata</i> enhanced plasma glucose and triacylglycerols values as well as hepatic substrate energy mobilization. Additionally, cortisol stimulated hepatic carbohydrates metabolism, as seen by the increased expression of metabolism-related genes. All of these responses, observed in cortisol-administered fish, were not detected by replicating the same protocol and instead using cortisol-BSA, which exclusively induces membrane-initiated effects. Therefore, we suggest that after three days of cortisol administration, only genomic actions are involved in the metabolic responses in <i>S. aurata</i>.https://www.mdpi.com/2076-2615/11/11/3310cortisolenergetic metabolismfishglucocorticoids<i>Sparus aurata</i>stress response |
| spellingShingle | Jorge Aedo Daniela Aravena-Canales Ignacio Ruiz-Jarabo Ricardo Oyarzún Alfredo Molina Gonzalo Martínez-Rodríguez Juan Antonio Valdés Juan Miguel Mancera Differential Metabolic and Transcriptional Responses of Gilthead Seabream (<i>Sparus aurata</i>) Administered with Cortisol or Cortisol-BSA Animals cortisol energetic metabolism fish glucocorticoids <i>Sparus aurata</i> stress response |
| title | Differential Metabolic and Transcriptional Responses of Gilthead Seabream (<i>Sparus aurata</i>) Administered with Cortisol or Cortisol-BSA |
| title_full | Differential Metabolic and Transcriptional Responses of Gilthead Seabream (<i>Sparus aurata</i>) Administered with Cortisol or Cortisol-BSA |
| title_fullStr | Differential Metabolic and Transcriptional Responses of Gilthead Seabream (<i>Sparus aurata</i>) Administered with Cortisol or Cortisol-BSA |
| title_full_unstemmed | Differential Metabolic and Transcriptional Responses of Gilthead Seabream (<i>Sparus aurata</i>) Administered with Cortisol or Cortisol-BSA |
| title_short | Differential Metabolic and Transcriptional Responses of Gilthead Seabream (<i>Sparus aurata</i>) Administered with Cortisol or Cortisol-BSA |
| title_sort | differential metabolic and transcriptional responses of gilthead seabream i sparus aurata i administered with cortisol or cortisol bsa |
| topic | cortisol energetic metabolism fish glucocorticoids <i>Sparus aurata</i> stress response |
| url | https://www.mdpi.com/2076-2615/11/11/3310 |
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