High Prevalence of GES-5 Variant and Co-Expression of VIM-2 and GES-45 among Clinical <i>Pseudomonas aeruginosa</i> Strains in Tunisia
Carbapenem-resistant <i>Pseudomonas aeruginosa</i> (CRPA) are a global health concern. The antimicrobial resistance, virulence, and molecular typing of 57 CRPA isolated from 43 patients who attended a specific Tunisian hospital from September 2018 to July 2019 were analyzed. All but one...
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| Format: | Article |
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MDPI AG
2023-08-01
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| Series: | Antibiotics |
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| Online Access: | https://www.mdpi.com/2079-6382/12/9/1394 |
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| author | Meha Fethi Beatriz Rojo-Bezares Ameni Arfaoui Raoudha Dziri Gabriela Chichón Farouk Barguellil María López Mohamed Selim El Asli Paula Toledano Hadda-Imen Ouzari Yolanda Sáenz Naouel Klibi |
| author_facet | Meha Fethi Beatriz Rojo-Bezares Ameni Arfaoui Raoudha Dziri Gabriela Chichón Farouk Barguellil María López Mohamed Selim El Asli Paula Toledano Hadda-Imen Ouzari Yolanda Sáenz Naouel Klibi |
| author_sort | Meha Fethi |
| collection | DOAJ |
| description | Carbapenem-resistant <i>Pseudomonas aeruginosa</i> (CRPA) are a global health concern. The antimicrobial resistance, virulence, and molecular typing of 57 CRPA isolated from 43 patients who attended a specific Tunisian hospital from September 2018 to July 2019 were analyzed. All but one were multidrug-resistant CRPA, and 77% were difficult-to-treat-resistant (DTR) isolates. The <i>bla</i><sub>VIM-2</sub> gene was detected in four strains (6.9%), and among the 36 <i>bla</i><sub>GES</sub>-positive CRPA (62%), the <i>bla</i><sub>GES-5</sub> gene was the predominant variant (86%). Three strains co-harbored the <i>bla</i><sub>VIM-2</sub> and <i>bla</i><sub>GES-45</sub> genes, and seven CRPA carried the <i>bla</i><sub>SHV-2a</sub> gene (14%). OprD alterations, including truncations by insertion sequences, were observed in 18 strains. Regarding the 46 class 1 integron-positive CRPA (81%), the <i>bla</i><sub>GES-5</sub> gene was located in integron In717, while the <i>bla</i><sub>GES-29</sub> and <i>bla</i><sub>GES-45</sub> genes were found in two new integrons (In2122 and In4879), and the <i>bla</i><sub>VIM-2</sub> gene was found in In1183 and the new integron In2142. Twenty-four PFGE patterns and thirteen sequence types (three new ones) were identified. The predominant serotype O:11 and <i>exoU</i> (81%) were mostly associated with ST235 and the new ST3385 clones. The seven <i>bla</i><sub>SHV-2a-</sub>CRPA from different patients belonged to ST3385 and the same PFGE pattern. The <i>bla</i><sub>GES-5</sub>- and <i>bla</i><sub>VIM-2</sub> + <i>bla</i><sub>GES-45</sub>-positive CRPA recovered mostly from ICU patients belonged to the high-risk clone ST235. Our results highlight the alarming prevalence of <i>bla</i><sub>GES-5-</sub> and ST235-CRPA, the co-existence of <i>bla</i><sub>GES-45</sub> and <i>bla</i><sub>VIM-2</sub>, and their location within integrons favoring their dissemination. |
| first_indexed | 2024-03-10T23:06:31Z |
| format | Article |
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| institution | Directory Open Access Journal |
| issn | 2079-6382 |
| language | English |
| last_indexed | 2024-03-10T23:06:31Z |
| publishDate | 2023-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Antibiotics |
| spelling | doaj.art-77712e4fe4c64aa48633c3fcf069b07a2023-11-19T09:17:10ZengMDPI AGAntibiotics2079-63822023-08-01129139410.3390/antibiotics12091394High Prevalence of GES-5 Variant and Co-Expression of VIM-2 and GES-45 among Clinical <i>Pseudomonas aeruginosa</i> Strains in TunisiaMeha Fethi0Beatriz Rojo-Bezares1Ameni Arfaoui2Raoudha Dziri3Gabriela Chichón4Farouk Barguellil5María López6Mohamed Selim El Asli7Paula Toledano8Hadda-Imen Ouzari9Yolanda Sáenz10Naouel Klibi11Laboratory of Microorganisms and Active Biomolecules, Faculty of Sciences of Tunis, University of Tunis El Manar, Tunis 2092, TunisiaÁrea de Microbiología Molecular, Centro de Investigación Biomédica de La Rioja (CIBIR), 26006 Logroño, SpainLaboratory of Microorganisms and Active Biomolecules, Faculty of Sciences of Tunis, University of Tunis El Manar, Tunis 2092, TunisiaLaboratory of Microorganisms and Active Biomolecules, Faculty of Sciences of Tunis, University of Tunis El Manar, Tunis 2092, TunisiaÁrea de Microbiología Molecular, Centro de Investigación Biomédica de La Rioja (CIBIR), 26006 Logroño, SpainLaboratory of Bacteriology, Military Hospital of Tunis, Tunis 1008, TunisiaÁrea de Microbiología Molecular, Centro de Investigación Biomédica de La Rioja (CIBIR), 26006 Logroño, SpainLaboratory of Bacteriology, Military Hospital of Tunis, Tunis 1008, TunisiaÁrea de Microbiología Molecular, Centro de Investigación Biomédica de La Rioja (CIBIR), 26006 Logroño, SpainLaboratory of Microorganisms and Active Biomolecules, Faculty of Sciences of Tunis, University of Tunis El Manar, Tunis 2092, TunisiaÁrea de Microbiología Molecular, Centro de Investigación Biomédica de La Rioja (CIBIR), 26006 Logroño, SpainLaboratory of Microorganisms and Active Biomolecules, Faculty of Sciences of Tunis, University of Tunis El Manar, Tunis 2092, TunisiaCarbapenem-resistant <i>Pseudomonas aeruginosa</i> (CRPA) are a global health concern. The antimicrobial resistance, virulence, and molecular typing of 57 CRPA isolated from 43 patients who attended a specific Tunisian hospital from September 2018 to July 2019 were analyzed. All but one were multidrug-resistant CRPA, and 77% were difficult-to-treat-resistant (DTR) isolates. The <i>bla</i><sub>VIM-2</sub> gene was detected in four strains (6.9%), and among the 36 <i>bla</i><sub>GES</sub>-positive CRPA (62%), the <i>bla</i><sub>GES-5</sub> gene was the predominant variant (86%). Three strains co-harbored the <i>bla</i><sub>VIM-2</sub> and <i>bla</i><sub>GES-45</sub> genes, and seven CRPA carried the <i>bla</i><sub>SHV-2a</sub> gene (14%). OprD alterations, including truncations by insertion sequences, were observed in 18 strains. Regarding the 46 class 1 integron-positive CRPA (81%), the <i>bla</i><sub>GES-5</sub> gene was located in integron In717, while the <i>bla</i><sub>GES-29</sub> and <i>bla</i><sub>GES-45</sub> genes were found in two new integrons (In2122 and In4879), and the <i>bla</i><sub>VIM-2</sub> gene was found in In1183 and the new integron In2142. Twenty-four PFGE patterns and thirteen sequence types (three new ones) were identified. The predominant serotype O:11 and <i>exoU</i> (81%) were mostly associated with ST235 and the new ST3385 clones. The seven <i>bla</i><sub>SHV-2a-</sub>CRPA from different patients belonged to ST3385 and the same PFGE pattern. The <i>bla</i><sub>GES-5</sub>- and <i>bla</i><sub>VIM-2</sub> + <i>bla</i><sub>GES-45</sub>-positive CRPA recovered mostly from ICU patients belonged to the high-risk clone ST235. Our results highlight the alarming prevalence of <i>bla</i><sub>GES-5-</sub> and ST235-CRPA, the co-existence of <i>bla</i><sub>GES-45</sub> and <i>bla</i><sub>VIM-2</sub>, and their location within integrons favoring their dissemination.https://www.mdpi.com/2079-6382/12/9/1394carbapenem resistanceGES-5class 1 integronhigh-risk clone ST235 |
| spellingShingle | Meha Fethi Beatriz Rojo-Bezares Ameni Arfaoui Raoudha Dziri Gabriela Chichón Farouk Barguellil María López Mohamed Selim El Asli Paula Toledano Hadda-Imen Ouzari Yolanda Sáenz Naouel Klibi High Prevalence of GES-5 Variant and Co-Expression of VIM-2 and GES-45 among Clinical <i>Pseudomonas aeruginosa</i> Strains in Tunisia Antibiotics carbapenem resistance GES-5 class 1 integron high-risk clone ST235 |
| title | High Prevalence of GES-5 Variant and Co-Expression of VIM-2 and GES-45 among Clinical <i>Pseudomonas aeruginosa</i> Strains in Tunisia |
| title_full | High Prevalence of GES-5 Variant and Co-Expression of VIM-2 and GES-45 among Clinical <i>Pseudomonas aeruginosa</i> Strains in Tunisia |
| title_fullStr | High Prevalence of GES-5 Variant and Co-Expression of VIM-2 and GES-45 among Clinical <i>Pseudomonas aeruginosa</i> Strains in Tunisia |
| title_full_unstemmed | High Prevalence of GES-5 Variant and Co-Expression of VIM-2 and GES-45 among Clinical <i>Pseudomonas aeruginosa</i> Strains in Tunisia |
| title_short | High Prevalence of GES-5 Variant and Co-Expression of VIM-2 and GES-45 among Clinical <i>Pseudomonas aeruginosa</i> Strains in Tunisia |
| title_sort | high prevalence of ges 5 variant and co expression of vim 2 and ges 45 among clinical i pseudomonas aeruginosa i strains in tunisia |
| topic | carbapenem resistance GES-5 class 1 integron high-risk clone ST235 |
| url | https://www.mdpi.com/2079-6382/12/9/1394 |
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