High prevalence of β-lactam and fluoroquinolone resistance in various phylotypes of Escherichia coli isolates from urinary tract infections in Jiroft city, Iran
Abstract Background Urinary tract infection (UTI) is one of the most prevalent infectious diseases with worldwide health threatening. Antimicrobial resistant strains of Escherichia coli (E. coli) are a common cause of UTI which were identified as a treatment challenge. This study aimed to assay the...
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| Format: | Article |
| Language: | English |
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BMC
2023-04-01
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| Series: | BMC Microbiology |
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| Online Access: | https://doi.org/10.1186/s12866-023-02860-7 |
| _version_ | 1797841151578865664 |
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| author | Saleh Afsharikhah Reza Ghanbarpour Parvin Mohseni Nasrin Adib Mahboube Bagheri Maziar Jajarmi |
| author_facet | Saleh Afsharikhah Reza Ghanbarpour Parvin Mohseni Nasrin Adib Mahboube Bagheri Maziar Jajarmi |
| author_sort | Saleh Afsharikhah |
| collection | DOAJ |
| description | Abstract Background Urinary tract infection (UTI) is one of the most prevalent infectious diseases with worldwide health threatening. Antimicrobial resistant strains of Escherichia coli (E. coli) are a common cause of UTI which were identified as a treatment challenge. This study aimed to assay the prevalence of common β-lactam resistance genes including bla TEM, bla SHV, bla CTX-M and bla CMY and phenotypic resistance to commonly used β-lactam and fluoroquinolone antibiotics in UTIs. These factors were evaluated in various phylogenetic groups (phylotypes) of E. coli isolates. Real-time PCR was applied to detect β-lactam resistance genes and conventional PCR was used to determine the phylotypes. Phenotypic resistance against β-lactams (ceftazidime, cefotaxime, aztreonam and ceftriaxone) and fluoroquinolones (ciprofloxacin) were identified by the disc diffusion technique. The ability of extended spectrum β-lactamases (ESBLs) production in E. coli isolates was detected using the combined disc diffusion method. Results The prevalence of resistance genes were 89.6% for bla TEM, 44.3% for bla CTX-M, 6.6% for bla SHV and 0.9% for bla CMY. The two high prevalent phylotypes were B2 (29.2%) and D (17.9%) followed by E (14.1%), F (9.4%), C (6.6%) and 10.3% of isolates were unknown in phylotyping. Disc diffusion results showed high prevalence of antibiotic resistance to cefotaxime (88.6%), aztreonam (83%), ceftireaxon (77.3%), ceftazidime (76.4%) and ciprofloxacin (55.6%). Totally, 52.8% of isolates were found as phenotypical ESBL-producers. Conclusions This study’s results confirmed an explosion of antibiotic resistance amongst E. coli isolates from UTI against β-lactams and fluoroquinolones. Findings explain the necessity of deep changes in quantity and quality of drug resistance diagnosis and antibiotic therapy strategies. More studies are suggested to better and confident evaluations. |
| first_indexed | 2024-04-09T16:26:26Z |
| format | Article |
| id | doaj.art-7771d580fe4148b3b27993d40d906673 |
| institution | Directory Open Access Journal |
| issn | 1471-2180 |
| language | English |
| last_indexed | 2024-04-09T16:26:26Z |
| publishDate | 2023-04-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Microbiology |
| spelling | doaj.art-7771d580fe4148b3b27993d40d9066732023-04-23T11:10:57ZengBMCBMC Microbiology1471-21802023-04-0123111210.1186/s12866-023-02860-7High prevalence of β-lactam and fluoroquinolone resistance in various phylotypes of Escherichia coli isolates from urinary tract infections in Jiroft city, IranSaleh Afsharikhah0Reza Ghanbarpour1Parvin Mohseni2Nasrin Adib3Mahboube Bagheri4Maziar Jajarmi5Department of Pathobiology, Faculty of Veterinary Medicine, Shahid Bahonar University of KermanMolecular Microbiology Research Group, Shahid Bahonar University of KermanDepartment of Pathobiology, Faculty of Veterinary Medicine, Shahid Bahonar University of KermanDepartment of Pathobiology, Faculty of Veterinary Medicine, Shahid Bahonar University of KermanDepartment of Food Science and Technology, Bardsir Faculty of Agriculture, Shahid Bahonar University of KermanDepartment of Pathobiology, Faculty of Veterinary Medicine, Shahid Bahonar University of KermanAbstract Background Urinary tract infection (UTI) is one of the most prevalent infectious diseases with worldwide health threatening. Antimicrobial resistant strains of Escherichia coli (E. coli) are a common cause of UTI which were identified as a treatment challenge. This study aimed to assay the prevalence of common β-lactam resistance genes including bla TEM, bla SHV, bla CTX-M and bla CMY and phenotypic resistance to commonly used β-lactam and fluoroquinolone antibiotics in UTIs. These factors were evaluated in various phylogenetic groups (phylotypes) of E. coli isolates. Real-time PCR was applied to detect β-lactam resistance genes and conventional PCR was used to determine the phylotypes. Phenotypic resistance against β-lactams (ceftazidime, cefotaxime, aztreonam and ceftriaxone) and fluoroquinolones (ciprofloxacin) were identified by the disc diffusion technique. The ability of extended spectrum β-lactamases (ESBLs) production in E. coli isolates was detected using the combined disc diffusion method. Results The prevalence of resistance genes were 89.6% for bla TEM, 44.3% for bla CTX-M, 6.6% for bla SHV and 0.9% for bla CMY. The two high prevalent phylotypes were B2 (29.2%) and D (17.9%) followed by E (14.1%), F (9.4%), C (6.6%) and 10.3% of isolates were unknown in phylotyping. Disc diffusion results showed high prevalence of antibiotic resistance to cefotaxime (88.6%), aztreonam (83%), ceftireaxon (77.3%), ceftazidime (76.4%) and ciprofloxacin (55.6%). Totally, 52.8% of isolates were found as phenotypical ESBL-producers. Conclusions This study’s results confirmed an explosion of antibiotic resistance amongst E. coli isolates from UTI against β-lactams and fluoroquinolones. Findings explain the necessity of deep changes in quantity and quality of drug resistance diagnosis and antibiotic therapy strategies. More studies are suggested to better and confident evaluations.https://doi.org/10.1186/s12866-023-02860-7Escherichia coliAntibiotic resistanceUTI |
| spellingShingle | Saleh Afsharikhah Reza Ghanbarpour Parvin Mohseni Nasrin Adib Mahboube Bagheri Maziar Jajarmi High prevalence of β-lactam and fluoroquinolone resistance in various phylotypes of Escherichia coli isolates from urinary tract infections in Jiroft city, Iran BMC Microbiology Escherichia coli Antibiotic resistance UTI |
| title | High prevalence of β-lactam and fluoroquinolone resistance in various phylotypes of Escherichia coli isolates from urinary tract infections in Jiroft city, Iran |
| title_full | High prevalence of β-lactam and fluoroquinolone resistance in various phylotypes of Escherichia coli isolates from urinary tract infections in Jiroft city, Iran |
| title_fullStr | High prevalence of β-lactam and fluoroquinolone resistance in various phylotypes of Escherichia coli isolates from urinary tract infections in Jiroft city, Iran |
| title_full_unstemmed | High prevalence of β-lactam and fluoroquinolone resistance in various phylotypes of Escherichia coli isolates from urinary tract infections in Jiroft city, Iran |
| title_short | High prevalence of β-lactam and fluoroquinolone resistance in various phylotypes of Escherichia coli isolates from urinary tract infections in Jiroft city, Iran |
| title_sort | high prevalence of β lactam and fluoroquinolone resistance in various phylotypes of escherichia coli isolates from urinary tract infections in jiroft city iran |
| topic | Escherichia coli Antibiotic resistance UTI |
| url | https://doi.org/10.1186/s12866-023-02860-7 |
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