Comparison of real-time reverse transcriptase polymerase chain reaction (RT-PCR) and IgM and IgG antibody test for the diagnosis of SARS-CoV-2 infection
In the present study, we compared the real-time reverse-transcription polymerase chain reaction (RT-PCR) and total IgM-IgG antibody tests for diagnosis SARS-CoV-2 patients. SARS-CoV-2 patients were divided into four additional subgroups according to clinical examination, and Computed Tomography (CT)...
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| Format: | Article |
| Language: | Indonesian |
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Poltekkes Kemenkes Yogyakarta
2020-08-01
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| Series: | Jurnal Teknologi Laboratorium |
| Subjects: | |
| Online Access: | https://teknolabjournal.com/index.php/Jtl/article/view/232 |
| _version_ | 1819228261913198592 |
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| author | Ömer ACER Osman ÖZÜDOĞRU |
| author_facet | Ömer ACER Osman ÖZÜDOĞRU |
| author_sort | Ömer ACER |
| collection | DOAJ |
| description | In the present study, we compared the real-time reverse-transcription polymerase chain reaction (RT-PCR) and total IgM-IgG antibody tests for diagnosis SARS-CoV-2 patients. SARS-CoV-2 patients were divided into four additional subgroups according to clinical examination, and Computed Tomography (CT) for SARS-CoV-2. Groups were included 60 mild cases, 111 moderate cases, 53 severe cases, and 105 normal cases. In a mild group, 52.5% of 60 cases were found to be male, 45.9% female, and the average age was found as 38.4 ±2.011. The positive ratio was found as 80.3% in the RT-PCR test, while 39.3% in total IgM/IgG. In a moderate group, 49.1% of 111 cases were found to be male, 50% female, and the average age was 45.05 ±1.519. The positive ratio was found as 85.7% in the RT-PCR test while 54.5% in total IgM/IgG. In a severe group, 53.7%of 53 cases were found to be male, 44.4% female, and the average age was 55.5±2.122. The positive ratio was found as 75.9% both in RT-PCR and total IgM/IgG tests. In a normal group with no involvement according to Computed Tomography (CT), 49.1%of 105 cases were found to be male, 50% female, and the average age was found as 34.8±1.391. The positive ratio was found as 95.3% in the RT-PCR test while 5.7% in total IgM/IgG. Chronic diseases were detected more in severe cases, suggesting that persons who have chronic diseases or decreased immunity, such as diabetes mellitus, cardiovascular diseases, hypertension, and lung disease, are at a higher risk for developing severe COVID-19 if they are infected with SARS-CoV-2. According to our results, we can suggest a higher detection sensibility in RT-PCR than in total IgM/IgG antibody test for mild, moderate and normal group, while the detection sensibility of IgM/IgG antibody increases in a severe group with bilateral diffuse involvement according to CT. |
| first_indexed | 2024-12-23T10:54:29Z |
| format | Article |
| id | doaj.art-7776081aebcd4ee78d1fa05a5d838525 |
| institution | Directory Open Access Journal |
| issn | 2338-5634 2580-0191 |
| language | Indonesian |
| last_indexed | 2024-12-23T10:54:29Z |
| publishDate | 2020-08-01 |
| publisher | Poltekkes Kemenkes Yogyakarta |
| record_format | Article |
| series | Jurnal Teknologi Laboratorium |
| spelling | doaj.art-7776081aebcd4ee78d1fa05a5d8385252022-12-21T17:49:48ZindPoltekkes Kemenkes YogyakartaJurnal Teknologi Laboratorium2338-56342580-01912020-08-0191788610.29238/teknolabjournal.v9i1.232232Comparison of real-time reverse transcriptase polymerase chain reaction (RT-PCR) and IgM and IgG antibody test for the diagnosis of SARS-CoV-2 infectionÖmer ACER0Osman ÖZÜDOĞRU1Department of Medical Microbiology, Medical Faculty, Siirt University, 56100, Siirt, TurkeySiirt Training and Research Hospital, Internal Medicine Clinic, 56100 Siirt, TurkeyIn the present study, we compared the real-time reverse-transcription polymerase chain reaction (RT-PCR) and total IgM-IgG antibody tests for diagnosis SARS-CoV-2 patients. SARS-CoV-2 patients were divided into four additional subgroups according to clinical examination, and Computed Tomography (CT) for SARS-CoV-2. Groups were included 60 mild cases, 111 moderate cases, 53 severe cases, and 105 normal cases. In a mild group, 52.5% of 60 cases were found to be male, 45.9% female, and the average age was found as 38.4 ±2.011. The positive ratio was found as 80.3% in the RT-PCR test, while 39.3% in total IgM/IgG. In a moderate group, 49.1% of 111 cases were found to be male, 50% female, and the average age was 45.05 ±1.519. The positive ratio was found as 85.7% in the RT-PCR test while 54.5% in total IgM/IgG. In a severe group, 53.7%of 53 cases were found to be male, 44.4% female, and the average age was 55.5±2.122. The positive ratio was found as 75.9% both in RT-PCR and total IgM/IgG tests. In a normal group with no involvement according to Computed Tomography (CT), 49.1%of 105 cases were found to be male, 50% female, and the average age was found as 34.8±1.391. The positive ratio was found as 95.3% in the RT-PCR test while 5.7% in total IgM/IgG. Chronic diseases were detected more in severe cases, suggesting that persons who have chronic diseases or decreased immunity, such as diabetes mellitus, cardiovascular diseases, hypertension, and lung disease, are at a higher risk for developing severe COVID-19 if they are infected with SARS-CoV-2. According to our results, we can suggest a higher detection sensibility in RT-PCR than in total IgM/IgG antibody test for mild, moderate and normal group, while the detection sensibility of IgM/IgG antibody increases in a severe group with bilateral diffuse involvement according to CT.https://teknolabjournal.com/index.php/Jtl/article/view/232sars-cov-2rt-pcrigm antibody testigg antibody test |
| spellingShingle | Ömer ACER Osman ÖZÜDOĞRU Comparison of real-time reverse transcriptase polymerase chain reaction (RT-PCR) and IgM and IgG antibody test for the diagnosis of SARS-CoV-2 infection Jurnal Teknologi Laboratorium sars-cov-2 rt-pcr igm antibody test igg antibody test |
| title | Comparison of real-time reverse transcriptase polymerase chain reaction (RT-PCR) and IgM and IgG antibody test for the diagnosis of SARS-CoV-2 infection |
| title_full | Comparison of real-time reverse transcriptase polymerase chain reaction (RT-PCR) and IgM and IgG antibody test for the diagnosis of SARS-CoV-2 infection |
| title_fullStr | Comparison of real-time reverse transcriptase polymerase chain reaction (RT-PCR) and IgM and IgG antibody test for the diagnosis of SARS-CoV-2 infection |
| title_full_unstemmed | Comparison of real-time reverse transcriptase polymerase chain reaction (RT-PCR) and IgM and IgG antibody test for the diagnosis of SARS-CoV-2 infection |
| title_short | Comparison of real-time reverse transcriptase polymerase chain reaction (RT-PCR) and IgM and IgG antibody test for the diagnosis of SARS-CoV-2 infection |
| title_sort | comparison of real time reverse transcriptase polymerase chain reaction rt pcr and igm and igg antibody test for the diagnosis of sars cov 2 infection |
| topic | sars-cov-2 rt-pcr igm antibody test igg antibody test |
| url | https://teknolabjournal.com/index.php/Jtl/article/view/232 |
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