The Viability of Single Cancer Cells after Exposure to Hydrodynamic Shear Stresses in a Spiral Microchannel: A Canine Cutaneous Mast Cell Tumor Model
Our laboratory has the fundamental responsibility to study cancer stem cells (CSC) in various models of human and animal neoplasms. However, the major impediments that spike our accomplishment are the lack of universal biomarkers and cellular heterogeneity. To cope with these restrictions, we have t...
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| Format: | Article |
| Language: | English |
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MDPI AG
2017-12-01
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| Series: | Micromachines |
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| Online Access: | https://www.mdpi.com/2072-666X/9/1/9 |
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| author | Dettachai Ketpun Achariya Sailasuta Thammawit Suwannaphan Sudchaya Bhanpattanakul Alongkorn Pimpin Werayut Srituravanich Witsaroot Sripumkhai Wutthinan Jeamsaksiri Prapruddee Piyaviriyakul |
| author_facet | Dettachai Ketpun Achariya Sailasuta Thammawit Suwannaphan Sudchaya Bhanpattanakul Alongkorn Pimpin Werayut Srituravanich Witsaroot Sripumkhai Wutthinan Jeamsaksiri Prapruddee Piyaviriyakul |
| author_sort | Dettachai Ketpun |
| collection | DOAJ |
| description | Our laboratory has the fundamental responsibility to study cancer stem cells (CSC) in various models of human and animal neoplasms. However, the major impediments that spike our accomplishment are the lack of universal biomarkers and cellular heterogeneity. To cope with these restrictions, we have tried to apply the concept of single cell analysis, which has hitherto been recommended throughout the world as an imperative solution pack for resolving such dilemmas. Accordingly, our first step was to utilize a predesigned spiral microchannel fabricated by our laboratory to perform size-based single cell separation using mast cell tumor (MCT) cells as a model. However, the impact of hydrodynamic shear stresses (HSS) on mechanical cell injury and viability in a spiral microchannel has not been fully investigated so far. Intuitively, our computational fluid dynamics (CFD) simulation has strongly revealed the formations of fluid shear stress (FSS) and extensional fluid stress (EFS) in the sorting system. The panel of biomedical assays has also disclosed cell degeneration and necrosis in the model. Therefore, we have herein reported the combinatorically detrimental effect of FSS and EFS on the viability of MCT cells after sorting in our spiral microchannel, with discussion on the possibly pathogenic mechanisms of HSS-induced cell injury in the study model. |
| first_indexed | 2024-12-19T22:25:37Z |
| format | Article |
| id | doaj.art-777a13536d314df3bee545aa427c99ae |
| institution | Directory Open Access Journal |
| issn | 2072-666X |
| language | English |
| last_indexed | 2024-12-19T22:25:37Z |
| publishDate | 2017-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Micromachines |
| spelling | doaj.art-777a13536d314df3bee545aa427c99ae2022-12-21T20:03:32ZengMDPI AGMicromachines2072-666X2017-12-0191910.3390/mi9010009mi9010009The Viability of Single Cancer Cells after Exposure to Hydrodynamic Shear Stresses in a Spiral Microchannel: A Canine Cutaneous Mast Cell Tumor ModelDettachai Ketpun0Achariya Sailasuta1Thammawit Suwannaphan2Sudchaya Bhanpattanakul3Alongkorn Pimpin4Werayut Srituravanich5Witsaroot Sripumkhai6Wutthinan Jeamsaksiri7Prapruddee Piyaviriyakul8Biochemistry Unit, Department of Physiology, Faculty of Veterinary Science, Chulalongkorn University, Bangkok 10330, ThailandCompanion Animal Cancer-Research Unit (CAC-RU), Department of Pathology, Faculty of Veterinary Science, Chulalongkorn University, Bangkok 10330, ThailandDepartment of Mechanical Engineering, Faculty of Engineering, Chulalongkorn University, Bangkok 10330, ThailandCompanion Animal Cancer-Research Unit (CAC-RU), Department of Pathology, Faculty of Veterinary Science, Chulalongkorn University, Bangkok 10330, ThailandCompanion Animal Cancer-Research Unit (CAC-RU), Department of Pathology, Faculty of Veterinary Science, Chulalongkorn University, Bangkok 10330, ThailandCompanion Animal Cancer-Research Unit (CAC-RU), Department of Pathology, Faculty of Veterinary Science, Chulalongkorn University, Bangkok 10330, ThailandThai Microelectronic Centre, Ministry of Science and Technology, Chachoengsao 24000, ThailandThai Microelectronic Centre, Ministry of Science and Technology, Chachoengsao 24000, ThailandBiochemistry Unit, Department of Physiology, Faculty of Veterinary Science, Chulalongkorn University, Bangkok 10330, ThailandOur laboratory has the fundamental responsibility to study cancer stem cells (CSC) in various models of human and animal neoplasms. However, the major impediments that spike our accomplishment are the lack of universal biomarkers and cellular heterogeneity. To cope with these restrictions, we have tried to apply the concept of single cell analysis, which has hitherto been recommended throughout the world as an imperative solution pack for resolving such dilemmas. Accordingly, our first step was to utilize a predesigned spiral microchannel fabricated by our laboratory to perform size-based single cell separation using mast cell tumor (MCT) cells as a model. However, the impact of hydrodynamic shear stresses (HSS) on mechanical cell injury and viability in a spiral microchannel has not been fully investigated so far. Intuitively, our computational fluid dynamics (CFD) simulation has strongly revealed the formations of fluid shear stress (FSS) and extensional fluid stress (EFS) in the sorting system. The panel of biomedical assays has also disclosed cell degeneration and necrosis in the model. Therefore, we have herein reported the combinatorically detrimental effect of FSS and EFS on the viability of MCT cells after sorting in our spiral microchannel, with discussion on the possibly pathogenic mechanisms of HSS-induced cell injury in the study model.https://www.mdpi.com/2072-666X/9/1/9hydrodynamic shear stressmicrofluidicmast cell tumorsortingspiral microchannelviability |
| spellingShingle | Dettachai Ketpun Achariya Sailasuta Thammawit Suwannaphan Sudchaya Bhanpattanakul Alongkorn Pimpin Werayut Srituravanich Witsaroot Sripumkhai Wutthinan Jeamsaksiri Prapruddee Piyaviriyakul The Viability of Single Cancer Cells after Exposure to Hydrodynamic Shear Stresses in a Spiral Microchannel: A Canine Cutaneous Mast Cell Tumor Model Micromachines hydrodynamic shear stress microfluidic mast cell tumor sorting spiral microchannel viability |
| title | The Viability of Single Cancer Cells after Exposure to Hydrodynamic Shear Stresses in a Spiral Microchannel: A Canine Cutaneous Mast Cell Tumor Model |
| title_full | The Viability of Single Cancer Cells after Exposure to Hydrodynamic Shear Stresses in a Spiral Microchannel: A Canine Cutaneous Mast Cell Tumor Model |
| title_fullStr | The Viability of Single Cancer Cells after Exposure to Hydrodynamic Shear Stresses in a Spiral Microchannel: A Canine Cutaneous Mast Cell Tumor Model |
| title_full_unstemmed | The Viability of Single Cancer Cells after Exposure to Hydrodynamic Shear Stresses in a Spiral Microchannel: A Canine Cutaneous Mast Cell Tumor Model |
| title_short | The Viability of Single Cancer Cells after Exposure to Hydrodynamic Shear Stresses in a Spiral Microchannel: A Canine Cutaneous Mast Cell Tumor Model |
| title_sort | viability of single cancer cells after exposure to hydrodynamic shear stresses in a spiral microchannel a canine cutaneous mast cell tumor model |
| topic | hydrodynamic shear stress microfluidic mast cell tumor sorting spiral microchannel viability |
| url | https://www.mdpi.com/2072-666X/9/1/9 |
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