Whole-genome sequencing reveals high-risk clones of Pseudomonas aeruginosa in Guangdong, China
The ever-increasing prevalence of infections produced by multidrug-resistant or extensively drug-resistant Pseudomonas aeruginosa is commonly linked to a limited number of aptly-named epidemical ‘high-risk clones’ that are widespread among and within hospitals worldwide. The emergence of new potenti...
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| Format: | Article |
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Frontiers Media S.A.
2023-04-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2023.1117017/full |
| _version_ | 1797847229850976256 |
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| author | Yonggang Zhao Dingqiang Chen Boyang Ji Xingju Zhang Mikkel Anbo Lars Jelsbak |
| author_facet | Yonggang Zhao Dingqiang Chen Boyang Ji Xingju Zhang Mikkel Anbo Lars Jelsbak |
| author_sort | Yonggang Zhao |
| collection | DOAJ |
| description | The ever-increasing prevalence of infections produced by multidrug-resistant or extensively drug-resistant Pseudomonas aeruginosa is commonly linked to a limited number of aptly-named epidemical ‘high-risk clones’ that are widespread among and within hospitals worldwide. The emergence of new potential high-risk clone strains in hospitals highlights the need to better and further understand the underlying genetic mechanisms for their emergence and success. P. aeruginosa related high-risk clones have been sporadically found in China, their genome sequences have rarely been described. Therefore, the large-scale sequencing of multidrug-resistance high-risk clone strains will help us to understand the emergence and transmission of antibiotic resistances in P. aeruginosa high-risk clones. In this study, 212 P. aeruginosa strains were isolated from 2 tertiary hospitals within 3 years (2018–2020) in Guangdong Province, China. Whole-genome sequencing, multi-locus sequence typing (MLST) and antimicrobial susceptibility testing were applied to analyze the genomic epidemiology of P. aeruginosa in this region. We found that up to 130 (61.32%) of the isolates were shown to be multidrug resistant, and 196 (92.45%) isolates were Carbapenem-Resistant Pseudomonas aeruginosa. MLST analysis demonstrated high diversity of sequence types, and 18 reported international high-risk clones were identified. Furthermore, we discovered the co-presence of exoU and exoS genes in 5 collected strains. This study enhances insight into the regional research of molecular epidemiology and antimicrobial resistance of P. aeruginosa in China. The high diversity of clone types and regional genome characteristics can serve as a theoretical reference for public health policies and help guide measures for the prevention and control of P. aeruginosa resistance. |
| first_indexed | 2024-04-09T18:09:03Z |
| format | Article |
| id | doaj.art-777ac3d174c246c9a0dc751b68f02370 |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-04-09T18:09:03Z |
| publishDate | 2023-04-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-777ac3d174c246c9a0dc751b68f023702023-04-14T04:23:37ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2023-04-011410.3389/fmicb.2023.11170171117017Whole-genome sequencing reveals high-risk clones of Pseudomonas aeruginosa in Guangdong, ChinaYonggang Zhao0Dingqiang Chen1Boyang Ji2Xingju Zhang3Mikkel Anbo4Lars Jelsbak5Department of Biotechnology and Biomedicine, Technical University of Denmark, Kongens Lyngby, DenmarkDepartment of Laboratory Medicine, Microbiome Medicine Center, Zhujiang Hospital, Southern Medical University, Guangzhou, Guangdong, ChinaDepartment of Biology and Biological Engineering, Chalmers University of Technology, Gothenburg, SwedenBGI-Shenzhen, Shenzhen, ChinaDepartment of Biotechnology and Biomedicine, Technical University of Denmark, Kongens Lyngby, DenmarkDepartment of Biotechnology and Biomedicine, Technical University of Denmark, Kongens Lyngby, DenmarkThe ever-increasing prevalence of infections produced by multidrug-resistant or extensively drug-resistant Pseudomonas aeruginosa is commonly linked to a limited number of aptly-named epidemical ‘high-risk clones’ that are widespread among and within hospitals worldwide. The emergence of new potential high-risk clone strains in hospitals highlights the need to better and further understand the underlying genetic mechanisms for their emergence and success. P. aeruginosa related high-risk clones have been sporadically found in China, their genome sequences have rarely been described. Therefore, the large-scale sequencing of multidrug-resistance high-risk clone strains will help us to understand the emergence and transmission of antibiotic resistances in P. aeruginosa high-risk clones. In this study, 212 P. aeruginosa strains were isolated from 2 tertiary hospitals within 3 years (2018–2020) in Guangdong Province, China. Whole-genome sequencing, multi-locus sequence typing (MLST) and antimicrobial susceptibility testing were applied to analyze the genomic epidemiology of P. aeruginosa in this region. We found that up to 130 (61.32%) of the isolates were shown to be multidrug resistant, and 196 (92.45%) isolates were Carbapenem-Resistant Pseudomonas aeruginosa. MLST analysis demonstrated high diversity of sequence types, and 18 reported international high-risk clones were identified. Furthermore, we discovered the co-presence of exoU and exoS genes in 5 collected strains. This study enhances insight into the regional research of molecular epidemiology and antimicrobial resistance of P. aeruginosa in China. The high diversity of clone types and regional genome characteristics can serve as a theoretical reference for public health policies and help guide measures for the prevention and control of P. aeruginosa resistance.https://www.frontiersin.org/articles/10.3389/fmicb.2023.1117017/fullPseudomonas aeruginosahigh-risk clonemultidrug resistancegenomic epidemiologycarbapenem resistant |
| spellingShingle | Yonggang Zhao Dingqiang Chen Boyang Ji Xingju Zhang Mikkel Anbo Lars Jelsbak Whole-genome sequencing reveals high-risk clones of Pseudomonas aeruginosa in Guangdong, China Frontiers in Microbiology Pseudomonas aeruginosa high-risk clone multidrug resistance genomic epidemiology carbapenem resistant |
| title | Whole-genome sequencing reveals high-risk clones of Pseudomonas aeruginosa in Guangdong, China |
| title_full | Whole-genome sequencing reveals high-risk clones of Pseudomonas aeruginosa in Guangdong, China |
| title_fullStr | Whole-genome sequencing reveals high-risk clones of Pseudomonas aeruginosa in Guangdong, China |
| title_full_unstemmed | Whole-genome sequencing reveals high-risk clones of Pseudomonas aeruginosa in Guangdong, China |
| title_short | Whole-genome sequencing reveals high-risk clones of Pseudomonas aeruginosa in Guangdong, China |
| title_sort | whole genome sequencing reveals high risk clones of pseudomonas aeruginosa in guangdong china |
| topic | Pseudomonas aeruginosa high-risk clone multidrug resistance genomic epidemiology carbapenem resistant |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2023.1117017/full |
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