Altered Steroidome in Women with Gestational Diabetes Mellitus: Focus on Neuroactive and Immunomodulatory Steroids from the 24th Week of Pregnancy to Labor
Gestational diabetes mellitus (GDM) is a complication in pregnancy, but studies focused on the steroidome in patients with GDM are not available in the public domain. This article evaluates the steroidome in GDM+ and GDM− women and its changes from 24 weeks (± of gestation) to labor. The study inclu...
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| Format: | Article |
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MDPI AG
2021-11-01
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| Series: | Biomolecules |
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| Online Access: | https://www.mdpi.com/2218-273X/11/12/1746 |
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| author | Leona Ondřejíková Antonín Pařízek Patrik Šimják Daniela Vejražková Marta Velíková Kateřina Anderlová Michala Vosátková Hana Krejčí Michal Koucký Radmila Kancheva Michaela Dušková Markéta Vaňková Josef Bulant Martin Hill |
| author_facet | Leona Ondřejíková Antonín Pařízek Patrik Šimják Daniela Vejražková Marta Velíková Kateřina Anderlová Michala Vosátková Hana Krejčí Michal Koucký Radmila Kancheva Michaela Dušková Markéta Vaňková Josef Bulant Martin Hill |
| author_sort | Leona Ondřejíková |
| collection | DOAJ |
| description | Gestational diabetes mellitus (GDM) is a complication in pregnancy, but studies focused on the steroidome in patients with GDM are not available in the public domain. This article evaluates the steroidome in GDM+ and GDM− women and its changes from 24 weeks (± of gestation) to labor. The study included GDM+ (<i>n</i> = 44) and GDM− women (<i>n</i> = 33), in weeks 24–28, 30–36 of gestation and at labor and mixed umbilical blood after delivery. Steroidomic data (101 steroids quantified by GC-MS/MS) support the concept that the increasing diabetogenic effects with the approaching term are associated with mounting progesterone levels. The GDM+ group showed lower levels of testosterone (due to reduced AKR1C3 activity), estradiol (due to a shift from the HSD17B1 towards HSD17B2 activity), 7-oxygenated androgens (competing with cortisone for HSD11B1 and shifting the balance from diabetogenic cortisol towards the inactive cortisone), reduced activities of SRD5As, and CYP17A1 in the hydroxylase but higher CYP17A1 activity in the lyase step. With the approaching term, the authors found rising activities of CYP3A7, AKR1C1, CYP17A1 in its hydroxylase step, but a decline in its lyase step, rising conjugation of neuroinhibitory and pregnancy-stabilizing steroids and weakening AKR1D1 activity. |
| first_indexed | 2024-03-10T04:33:46Z |
| format | Article |
| id | doaj.art-777ada2d1aff49569eceecd413f1a7a2 |
| institution | Directory Open Access Journal |
| issn | 2218-273X |
| language | English |
| last_indexed | 2024-03-10T04:33:46Z |
| publishDate | 2021-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biomolecules |
| spelling | doaj.art-777ada2d1aff49569eceecd413f1a7a22023-11-23T03:58:30ZengMDPI AGBiomolecules2218-273X2021-11-011112174610.3390/biom11121746Altered Steroidome in Women with Gestational Diabetes Mellitus: Focus on Neuroactive and Immunomodulatory Steroids from the 24th Week of Pregnancy to LaborLeona Ondřejíková0Antonín Pařízek1Patrik Šimják2Daniela Vejražková3Marta Velíková4Kateřina Anderlová5Michala Vosátková6Hana Krejčí7Michal Koucký8Radmila Kancheva9Michaela Dušková10Markéta Vaňková11Josef Bulant12Martin Hill13Institute of Endocrinology, 116 94 Prague, Czech RepublicDepartment of Gynecology and Obstetrics, First Faculty of Medicine, General University Hospital in Prague, Charles University in Prague, 128 08 Prague, Czech RepublicDepartment of Gynecology and Obstetrics, First Faculty of Medicine, General University Hospital in Prague, Charles University in Prague, 128 08 Prague, Czech RepublicInstitute of Endocrinology, 116 94 Prague, Czech RepublicInstitute of Endocrinology, 116 94 Prague, Czech RepublicDepartment of Gynecology and Obstetrics, First Faculty of Medicine, General University Hospital in Prague, Charles University in Prague, 128 08 Prague, Czech RepublicInstitute of Endocrinology, 116 94 Prague, Czech RepublicDepartment of Gynecology and Obstetrics, First Faculty of Medicine, General University Hospital in Prague, Charles University in Prague, 128 08 Prague, Czech RepublicDepartment of Gynecology and Obstetrics, First Faculty of Medicine, General University Hospital in Prague, Charles University in Prague, 128 08 Prague, Czech RepublicInstitute of Endocrinology, 116 94 Prague, Czech RepublicInstitute of Endocrinology, 116 94 Prague, Czech RepublicInstitute of Endocrinology, 116 94 Prague, Czech RepublicInstitute of Endocrinology, 116 94 Prague, Czech RepublicInstitute of Endocrinology, 116 94 Prague, Czech RepublicGestational diabetes mellitus (GDM) is a complication in pregnancy, but studies focused on the steroidome in patients with GDM are not available in the public domain. This article evaluates the steroidome in GDM+ and GDM− women and its changes from 24 weeks (± of gestation) to labor. The study included GDM+ (<i>n</i> = 44) and GDM− women (<i>n</i> = 33), in weeks 24–28, 30–36 of gestation and at labor and mixed umbilical blood after delivery. Steroidomic data (101 steroids quantified by GC-MS/MS) support the concept that the increasing diabetogenic effects with the approaching term are associated with mounting progesterone levels. The GDM+ group showed lower levels of testosterone (due to reduced AKR1C3 activity), estradiol (due to a shift from the HSD17B1 towards HSD17B2 activity), 7-oxygenated androgens (competing with cortisone for HSD11B1 and shifting the balance from diabetogenic cortisol towards the inactive cortisone), reduced activities of SRD5As, and CYP17A1 in the hydroxylase but higher CYP17A1 activity in the lyase step. With the approaching term, the authors found rising activities of CYP3A7, AKR1C1, CYP17A1 in its hydroxylase step, but a decline in its lyase step, rising conjugation of neuroinhibitory and pregnancy-stabilizing steroids and weakening AKR1D1 activity.https://www.mdpi.com/2218-273X/11/12/1746gestational diabetes mellitussteroidomeneuroactive steroidsimmunoprotective steroidsgestational agematernal blood |
| spellingShingle | Leona Ondřejíková Antonín Pařízek Patrik Šimják Daniela Vejražková Marta Velíková Kateřina Anderlová Michala Vosátková Hana Krejčí Michal Koucký Radmila Kancheva Michaela Dušková Markéta Vaňková Josef Bulant Martin Hill Altered Steroidome in Women with Gestational Diabetes Mellitus: Focus on Neuroactive and Immunomodulatory Steroids from the 24th Week of Pregnancy to Labor Biomolecules gestational diabetes mellitus steroidome neuroactive steroids immunoprotective steroids gestational age maternal blood |
| title | Altered Steroidome in Women with Gestational Diabetes Mellitus: Focus on Neuroactive and Immunomodulatory Steroids from the 24th Week of Pregnancy to Labor |
| title_full | Altered Steroidome in Women with Gestational Diabetes Mellitus: Focus on Neuroactive and Immunomodulatory Steroids from the 24th Week of Pregnancy to Labor |
| title_fullStr | Altered Steroidome in Women with Gestational Diabetes Mellitus: Focus on Neuroactive and Immunomodulatory Steroids from the 24th Week of Pregnancy to Labor |
| title_full_unstemmed | Altered Steroidome in Women with Gestational Diabetes Mellitus: Focus on Neuroactive and Immunomodulatory Steroids from the 24th Week of Pregnancy to Labor |
| title_short | Altered Steroidome in Women with Gestational Diabetes Mellitus: Focus on Neuroactive and Immunomodulatory Steroids from the 24th Week of Pregnancy to Labor |
| title_sort | altered steroidome in women with gestational diabetes mellitus focus on neuroactive and immunomodulatory steroids from the 24th week of pregnancy to labor |
| topic | gestational diabetes mellitus steroidome neuroactive steroids immunoprotective steroids gestational age maternal blood |
| url | https://www.mdpi.com/2218-273X/11/12/1746 |
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