MiRNA-1976 Regulates the Apoptosis of Dopaminergic Neurons by Targeting the PINK1 Gene
Introduction: Parkinson’s disease (PD), which is a neurodegenerative disease, requires urgently needed biomarkers to explore its mechanism. We screened for differences in the expression of microRNAs (miRNAs) and identified miR-1976 as a possible biomarker. Methods: Twenty-three patients and 30 contr...
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| Format: | Article |
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IMR Press
2023-02-01
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| Series: | Journal of Integrative Neuroscience |
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| Online Access: | https://www.imrpress.com/journal/JIN/22/2/10.31083/j.jin2202045 |
| _version_ | 1827976665221824512 |
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| author | Feng Qiu Yue Wu Guojin Xie Hui Cao Mingyang Du Haibo Jiang |
| author_facet | Feng Qiu Yue Wu Guojin Xie Hui Cao Mingyang Du Haibo Jiang |
| author_sort | Feng Qiu |
| collection | DOAJ |
| description | Introduction: Parkinson’s disease (PD), which is a neurodegenerative disease, requires urgently needed biomarkers to explore its mechanism. We screened for differences in the expression of microRNAs (miRNAs) and identified miR-1976 as a possible biomarker. Methods: Twenty-three patients and 30 controls were included in this study. Dopaminergic neurons from C57/BL mice were cultured. The miRNA expression profiles were analyzed using an miRNA microarray. MiR-1976 was identified as an miRNA that was differentially expressed between PD patients and age-matched controls. Lentiviral vectors were constructed, then apoptosis in dopaminergic neurons was analyzed using MTS (multicellular tumor spheroids) and flow cytometry. Transfection of miR-1976 mimics into MES23.5 cells was performed, and target genes and biological effects were analyzed. Results: Overexpression of miR-1976 increased apoptosis and mitochondrial damage in dopaminergic neurons. PINK1 (PINK1-induced kinase 1) was the most common target protein of miR-1976, and silencing of PINK1 caused mitochondrial damage and increased apoptosis of MES23.5 cells. Conclusions: MiR-1976 is a newly discovered miRNA that exhibits a high degree of differential expression with respect to the apoptosis of dopaminergic neurons. Given these results, increased expression of miR-1976 may increase the risk of PD by targeting PINK1 and may therefore be a useful biomarker for PD. |
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| id | doaj.art-777b0b9b50fa412ebba6887e3138b4a6 |
| institution | Directory Open Access Journal |
| issn | 0219-6352 |
| language | English |
| last_indexed | 2024-04-09T20:39:07Z |
| publishDate | 2023-02-01 |
| publisher | IMR Press |
| record_format | Article |
| series | Journal of Integrative Neuroscience |
| spelling | doaj.art-777b0b9b50fa412ebba6887e3138b4a62023-03-30T05:58:08ZengIMR PressJournal of Integrative Neuroscience0219-63522023-02-012224510.31083/j.jin2202045S0219-6352(22)00464-8MiRNA-1976 Regulates the Apoptosis of Dopaminergic Neurons by Targeting the PINK1 GeneFeng Qiu0Yue Wu1Guojin Xie2Hui Cao3Mingyang Du4Haibo Jiang5Cerebrovascular Disease Center, Nanjing Brain Hospital Affiliated to Nanjing Medical University, 210029 Nanjing, Jiangsu, ChinaNeonatal Medical Center, Children’s Hospital of Nanjing Medical University, 210008 Nanjing, Jiangsu, ChinaDepartment of Clinical Laboratory, Children’s Hospital of Nanjing Medical University, 210008 Nanjing, Jiangsu, ChinaCerebrovascular Disease Center, Nanjing Brain Hospital Affiliated to Nanjing Medical University, 210029 Nanjing, Jiangsu, ChinaCerebrovascular Disease Center, Nanjing Brain Hospital Affiliated to Nanjing Medical University, 210029 Nanjing, Jiangsu, ChinaCerebrovascular Disease Center, Nanjing Brain Hospital Affiliated to Nanjing Medical University, 210029 Nanjing, Jiangsu, ChinaIntroduction: Parkinson’s disease (PD), which is a neurodegenerative disease, requires urgently needed biomarkers to explore its mechanism. We screened for differences in the expression of microRNAs (miRNAs) and identified miR-1976 as a possible biomarker. Methods: Twenty-three patients and 30 controls were included in this study. Dopaminergic neurons from C57/BL mice were cultured. The miRNA expression profiles were analyzed using an miRNA microarray. MiR-1976 was identified as an miRNA that was differentially expressed between PD patients and age-matched controls. Lentiviral vectors were constructed, then apoptosis in dopaminergic neurons was analyzed using MTS (multicellular tumor spheroids) and flow cytometry. Transfection of miR-1976 mimics into MES23.5 cells was performed, and target genes and biological effects were analyzed. Results: Overexpression of miR-1976 increased apoptosis and mitochondrial damage in dopaminergic neurons. PINK1 (PINK1-induced kinase 1) was the most common target protein of miR-1976, and silencing of PINK1 caused mitochondrial damage and increased apoptosis of MES23.5 cells. Conclusions: MiR-1976 is a newly discovered miRNA that exhibits a high degree of differential expression with respect to the apoptosis of dopaminergic neurons. Given these results, increased expression of miR-1976 may increase the risk of PD by targeting PINK1 and may therefore be a useful biomarker for PD.https://www.imrpress.com/journal/JIN/22/2/10.31083/j.jin2202045parkinson's diseasemir-1976pink1biomarkertarget proteindopaminergic neuronapoptosis |
| spellingShingle | Feng Qiu Yue Wu Guojin Xie Hui Cao Mingyang Du Haibo Jiang MiRNA-1976 Regulates the Apoptosis of Dopaminergic Neurons by Targeting the PINK1 Gene Journal of Integrative Neuroscience parkinson's disease mir-1976 pink1 biomarker target protein dopaminergic neuron apoptosis |
| title | MiRNA-1976 Regulates the Apoptosis of Dopaminergic Neurons by Targeting the PINK1 Gene |
| title_full | MiRNA-1976 Regulates the Apoptosis of Dopaminergic Neurons by Targeting the PINK1 Gene |
| title_fullStr | MiRNA-1976 Regulates the Apoptosis of Dopaminergic Neurons by Targeting the PINK1 Gene |
| title_full_unstemmed | MiRNA-1976 Regulates the Apoptosis of Dopaminergic Neurons by Targeting the PINK1 Gene |
| title_short | MiRNA-1976 Regulates the Apoptosis of Dopaminergic Neurons by Targeting the PINK1 Gene |
| title_sort | mirna 1976 regulates the apoptosis of dopaminergic neurons by targeting the pink1 gene |
| topic | parkinson's disease mir-1976 pink1 biomarker target protein dopaminergic neuron apoptosis |
| url | https://www.imrpress.com/journal/JIN/22/2/10.31083/j.jin2202045 |
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