Prevalence and genotyping distribution of Enterocytozoon bieneusi in diarrheic pigs in Chongqing and Sichuan provinces, China
The microsporidian fungal pathogen Enterocytozoon bieneusi is a unicellular parasite that infects humans and various animals, including pigs. Currently, there are few data on E. bieneusi infection a in diarrheic pigs in Chongqing and Sichuan Provinces, China. This study aims to determine the prevale...
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Frontiers Media S.A.
2022-10-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2022.1025613/full |
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| author | Samson Teweldeberhan Ghebremichael Samson Teweldeberhan Ghebremichael Samson Teweldeberhan Ghebremichael Xianzhi Meng Xianzhi Meng Junhong Wei Junhong Wei Yujiao Yang Qingyuan Huang Qingyuan Huang Lie Luo Lie Luo Heng Xiang Jie Chen Jie Chen M. A. Abo-Kadoum Tian Li Tian Li Xiao Liu Jialing Bao Jialing Bao Zeyang Zhou Zeyang Zhou Zeyang Zhou Guoqing Pan Guoqing Pan Guoqing Pan |
| author_facet | Samson Teweldeberhan Ghebremichael Samson Teweldeberhan Ghebremichael Samson Teweldeberhan Ghebremichael Xianzhi Meng Xianzhi Meng Junhong Wei Junhong Wei Yujiao Yang Qingyuan Huang Qingyuan Huang Lie Luo Lie Luo Heng Xiang Jie Chen Jie Chen M. A. Abo-Kadoum Tian Li Tian Li Xiao Liu Jialing Bao Jialing Bao Zeyang Zhou Zeyang Zhou Zeyang Zhou Guoqing Pan Guoqing Pan Guoqing Pan |
| author_sort | Samson Teweldeberhan Ghebremichael |
| collection | DOAJ |
| description | The microsporidian fungal pathogen Enterocytozoon bieneusi is a unicellular parasite that infects humans and various animals, including pigs. Currently, there are few data on E. bieneusi infection a in diarrheic pigs in Chongqing and Sichuan Provinces, China. This study aims to determine the prevalence and genotype distribution of E. bieneusi in diarrheic pigs. In total, 514 fecal samples from diarrheic pigs were obtained from 14 large-scale farms in Chongqing and Sichuan Provinces (326 suckling pigs, 17 weaned pigs, 65 fattening pigs, and 106 sows). To identify the E. bieneusi genotypes, genomic DNA was isolated from the samples and tested by nested PCR, targeting the internal transcribed spacer region of the rRNA followed by DNA sequence analysis. The overall prevalence of E. bieneusi was 79.8% (410/514), with rates of 84.9% (90/106) in sows and 64.7% (11/17) in weaned pigs. We found 61 different genotypes, including seven known genotypes (E, F, CHG1, Peru8, CAF1, B, and BEB17) and 54 novel genotypes. These 54 new genotypes are variants of eight known genotypes (SDD2, A, B, HLJD-IV, PigSpEb1, O, JLD-I, and BEB17) based on their sequence similarities. Phylogenetically, all of the identified genotypes clustered with counterparts belonging to Group 1 and Group 2 of E. bieneusi. Therefore, we found a higher prevalence of E. bieneusi in sows than in preweaned and weaned pigs. These findings indicate that diarrheic pigs could be a potential reservoir host, which can contaminate the environment and be a source of microsporidia in humans and other animals. |
| first_indexed | 2024-04-11T10:02:57Z |
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| last_indexed | 2024-04-11T10:02:57Z |
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| spelling | doaj.art-777b344b5e714de48235cf1cd62163f62022-12-22T04:30:19ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2022-10-011310.3389/fmicb.2022.10256131025613Prevalence and genotyping distribution of Enterocytozoon bieneusi in diarrheic pigs in Chongqing and Sichuan provinces, ChinaSamson Teweldeberhan Ghebremichael0Samson Teweldeberhan Ghebremichael1Samson Teweldeberhan Ghebremichael2Xianzhi Meng3Xianzhi Meng4Junhong Wei5Junhong Wei6Yujiao Yang7Qingyuan Huang8Qingyuan Huang9Lie Luo10Lie Luo11Heng Xiang12Jie Chen13Jie Chen14M. A. Abo-Kadoum15Tian Li16Tian Li17Xiao Liu18Jialing Bao19Jialing Bao20Zeyang Zhou21Zeyang Zhou22Zeyang Zhou23Guoqing Pan24Guoqing Pan25Guoqing Pan26State Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing, ChinaChongqing Key Laboratory of Microsporidia Infection and Control, Southwest University, Chongqing, ChinaDepartment of Biology, Mai Nefhi College of Science, Mai-Nefhi, EritreaState Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing, ChinaChongqing Key Laboratory of Microsporidia Infection and Control, Southwest University, Chongqing, ChinaState Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing, ChinaChongqing Key Laboratory of Microsporidia Infection and Control, Southwest University, Chongqing, ChinaCollege of Veterinary Medicine, Southwest University, Chongqing, ChinaState Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing, ChinaChongqing Key Laboratory of Microsporidia Infection and Control, Southwest University, Chongqing, ChinaState Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing, ChinaChongqing Key Laboratory of Microsporidia Infection and Control, Southwest University, Chongqing, ChinaCollege of Animal Science and Technology, Southwest University, Chongqing, ChinaState Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing, ChinaChongqing Key Laboratory of Microsporidia Infection and Control, Southwest University, Chongqing, ChinaBotany and Microbiology Department, Faculty of Science, Al-Azhar University, Assuit Branch, Cairo, EgyptState Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing, ChinaChongqing Key Laboratory of Microsporidia Infection and Control, Southwest University, Chongqing, ChinaCollege of Veterinary Medicine, Southwest University, Chongqing, ChinaState Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing, ChinaChongqing Key Laboratory of Microsporidia Infection and Control, Southwest University, Chongqing, ChinaState Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing, ChinaChongqing Key Laboratory of Microsporidia Infection and Control, Southwest University, Chongqing, ChinaCollege of Life Sciences, Chongqing Normal University, Chongqing, ChinaState Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing, ChinaChongqing Key Laboratory of Microsporidia Infection and Control, Southwest University, Chongqing, ChinaCollege of Life Sciences, Chongqing Normal University, Chongqing, ChinaThe microsporidian fungal pathogen Enterocytozoon bieneusi is a unicellular parasite that infects humans and various animals, including pigs. Currently, there are few data on E. bieneusi infection a in diarrheic pigs in Chongqing and Sichuan Provinces, China. This study aims to determine the prevalence and genotype distribution of E. bieneusi in diarrheic pigs. In total, 514 fecal samples from diarrheic pigs were obtained from 14 large-scale farms in Chongqing and Sichuan Provinces (326 suckling pigs, 17 weaned pigs, 65 fattening pigs, and 106 sows). To identify the E. bieneusi genotypes, genomic DNA was isolated from the samples and tested by nested PCR, targeting the internal transcribed spacer region of the rRNA followed by DNA sequence analysis. The overall prevalence of E. bieneusi was 79.8% (410/514), with rates of 84.9% (90/106) in sows and 64.7% (11/17) in weaned pigs. We found 61 different genotypes, including seven known genotypes (E, F, CHG1, Peru8, CAF1, B, and BEB17) and 54 novel genotypes. These 54 new genotypes are variants of eight known genotypes (SDD2, A, B, HLJD-IV, PigSpEb1, O, JLD-I, and BEB17) based on their sequence similarities. Phylogenetically, all of the identified genotypes clustered with counterparts belonging to Group 1 and Group 2 of E. bieneusi. Therefore, we found a higher prevalence of E. bieneusi in sows than in preweaned and weaned pigs. These findings indicate that diarrheic pigs could be a potential reservoir host, which can contaminate the environment and be a source of microsporidia in humans and other animals.https://www.frontiersin.org/articles/10.3389/fmicb.2022.1025613/fullprevalenceEnterocytozoon bieneusigenotypingdiarrheic pigsinternal transcribed spacernovel |
| spellingShingle | Samson Teweldeberhan Ghebremichael Samson Teweldeberhan Ghebremichael Samson Teweldeberhan Ghebremichael Xianzhi Meng Xianzhi Meng Junhong Wei Junhong Wei Yujiao Yang Qingyuan Huang Qingyuan Huang Lie Luo Lie Luo Heng Xiang Jie Chen Jie Chen M. A. Abo-Kadoum Tian Li Tian Li Xiao Liu Jialing Bao Jialing Bao Zeyang Zhou Zeyang Zhou Zeyang Zhou Guoqing Pan Guoqing Pan Guoqing Pan Prevalence and genotyping distribution of Enterocytozoon bieneusi in diarrheic pigs in Chongqing and Sichuan provinces, China Frontiers in Microbiology prevalence Enterocytozoon bieneusi genotyping diarrheic pigs internal transcribed spacer novel |
| title | Prevalence and genotyping distribution of Enterocytozoon bieneusi in diarrheic pigs in Chongqing and Sichuan provinces, China |
| title_full | Prevalence and genotyping distribution of Enterocytozoon bieneusi in diarrheic pigs in Chongqing and Sichuan provinces, China |
| title_fullStr | Prevalence and genotyping distribution of Enterocytozoon bieneusi in diarrheic pigs in Chongqing and Sichuan provinces, China |
| title_full_unstemmed | Prevalence and genotyping distribution of Enterocytozoon bieneusi in diarrheic pigs in Chongqing and Sichuan provinces, China |
| title_short | Prevalence and genotyping distribution of Enterocytozoon bieneusi in diarrheic pigs in Chongqing and Sichuan provinces, China |
| title_sort | prevalence and genotyping distribution of enterocytozoon bieneusi in diarrheic pigs in chongqing and sichuan provinces china |
| topic | prevalence Enterocytozoon bieneusi genotyping diarrheic pigs internal transcribed spacer novel |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2022.1025613/full |
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