Anatomopathological changes in canine distemper seropositive dogs and virus detection in sinoatrial nodes
Canine distemper is a viral disease that affects several systems on dogs, among them, the cardiovascular system. The aim of this study was to identify canine distemper virus (CDV) in the sinoatrial node (SAN) of dogs serologically positive for distemper by Polymerase Chain Reaction preceded by reve...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Universidade Federal de Uberlândia
2020-02-01
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| Series: | Bioscience Journal |
| Subjects: | |
| Online Access: | https://seer-dev.ufu.br/index.php/biosciencejournal/article/view/42122 |
| _version_ | 1818157120665681920 |
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| author | José Eugenio Diniz Bastos Neide Maria Silva Marisol Patrícia Pallete Briceño Tais Meziara Wilson Alessandra Aparecida Medeiros-Ronchi |
| author_facet | José Eugenio Diniz Bastos Neide Maria Silva Marisol Patrícia Pallete Briceño Tais Meziara Wilson Alessandra Aparecida Medeiros-Ronchi |
| author_sort | José Eugenio Diniz Bastos |
| collection | DOAJ |
| description |
Canine distemper is a viral disease that affects several systems on dogs, among them, the cardiovascular system. The aim of this study was to identify canine distemper virus (CDV) in the sinoatrial node (SAN) of dogs serologically positive for distemper by Polymerase Chain Reaction preceded by reverse transcription (RT-PCR), and to analyze gross and microscopic changes of distemper in the heart and other tissues. SAN and tissue fragments were collected from 17 serologically positive dead animals, necropsied from October 2015 to December 2016. In the heart, right heart dilatation was observed in 13 dogs (76.47%) and left concentric hypertrophy in two dogs (11.76%). Microscopically, lymphocytic myocarditis was observed in four (23.53%) dogs and 41.18% presented viral inclusion corpuscles of CDV in the bladder epithelium. Only one (5.88%) dog presented a 319 bp target fragment for distemper virus using primers CDV 1 and CDV 2 at the sinoatrial node. In conclusion, CDV can be located in the sinoatrial node of naturally infected dogs, as demonstrated in this study by the RT-PCR technique, reinforcing the hypothesis that CDV is capable of causing inflammatory lesions in the sinoatrial node of this species. Macroscopic and microscopic cardiac changes are frequently observed in dogs with distemper, mainly cardiac dilatation and myocarditis. Viral inclusions of CDV in bladder epithelial cells are an important microscopic finding for the diagnosis of distemper.
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| first_indexed | 2024-12-11T15:09:09Z |
| format | Article |
| id | doaj.art-777c8e0f2953438da626fe77bec91820 |
| institution | Directory Open Access Journal |
| issn | 1981-3163 |
| language | English |
| last_indexed | 2024-12-11T15:09:09Z |
| publishDate | 2020-02-01 |
| publisher | Universidade Federal de Uberlândia |
| record_format | Article |
| series | Bioscience Journal |
| spelling | doaj.art-777c8e0f2953438da626fe77bec918202022-12-22T01:00:49ZengUniversidade Federal de UberlândiaBioscience Journal1981-31632020-02-01362Anatomopathological changes in canine distemper seropositive dogs and virus detection in sinoatrial nodesJosé Eugenio Diniz Bastos0Neide Maria Silva1Marisol Patrícia Pallete Briceño2Tais Meziara Wilson3Alessandra Aparecida Medeiros-Ronchi4Universidade Federal de UberlândiaUniversidade Federal de UberlândiaUniversidade Federal de UberlândiaUniversidade de Brasília Universidade Federal de Uberlândia Canine distemper is a viral disease that affects several systems on dogs, among them, the cardiovascular system. The aim of this study was to identify canine distemper virus (CDV) in the sinoatrial node (SAN) of dogs serologically positive for distemper by Polymerase Chain Reaction preceded by reverse transcription (RT-PCR), and to analyze gross and microscopic changes of distemper in the heart and other tissues. SAN and tissue fragments were collected from 17 serologically positive dead animals, necropsied from October 2015 to December 2016. In the heart, right heart dilatation was observed in 13 dogs (76.47%) and left concentric hypertrophy in two dogs (11.76%). Microscopically, lymphocytic myocarditis was observed in four (23.53%) dogs and 41.18% presented viral inclusion corpuscles of CDV in the bladder epithelium. Only one (5.88%) dog presented a 319 bp target fragment for distemper virus using primers CDV 1 and CDV 2 at the sinoatrial node. In conclusion, CDV can be located in the sinoatrial node of naturally infected dogs, as demonstrated in this study by the RT-PCR technique, reinforcing the hypothesis that CDV is capable of causing inflammatory lesions in the sinoatrial node of this species. Macroscopic and microscopic cardiac changes are frequently observed in dogs with distemper, mainly cardiac dilatation and myocarditis. Viral inclusions of CDV in bladder epithelial cells are an important microscopic finding for the diagnosis of distemper. https://seer-dev.ufu.br/index.php/biosciencejournal/article/view/42122Cardiac Conduction SystemCanine Distemper VirusCardiomyopathy |
| spellingShingle | José Eugenio Diniz Bastos Neide Maria Silva Marisol Patrícia Pallete Briceño Tais Meziara Wilson Alessandra Aparecida Medeiros-Ronchi Anatomopathological changes in canine distemper seropositive dogs and virus detection in sinoatrial nodes Bioscience Journal Cardiac Conduction System Canine Distemper Virus Cardiomyopathy |
| title | Anatomopathological changes in canine distemper seropositive dogs and virus detection in sinoatrial nodes |
| title_full | Anatomopathological changes in canine distemper seropositive dogs and virus detection in sinoatrial nodes |
| title_fullStr | Anatomopathological changes in canine distemper seropositive dogs and virus detection in sinoatrial nodes |
| title_full_unstemmed | Anatomopathological changes in canine distemper seropositive dogs and virus detection in sinoatrial nodes |
| title_short | Anatomopathological changes in canine distemper seropositive dogs and virus detection in sinoatrial nodes |
| title_sort | anatomopathological changes in canine distemper seropositive dogs and virus detection in sinoatrial nodes |
| topic | Cardiac Conduction System Canine Distemper Virus Cardiomyopathy |
| url | https://seer-dev.ufu.br/index.php/biosciencejournal/article/view/42122 |
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