Molecular and phenotypic characterization of clinical isolates belonging to a KPC-2-producing strain of ST15 Klebsiella pneumoniae from a Vietnamese pediatric hospital
Abstract Background Carbapenem-resistant Klebsiella pneumoniae are becoming increasingly common in hospital settings worldwide and are a source of increased morbidity, mortality and health care costs. The global epidemiology of carbapenem-resistant K. pneumoniae is characterized by different strains...
| Main Authors: | , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2019-10-01
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| Series: | Antimicrobial Resistance and Infection Control |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s13756-019-0613-4 |
| _version_ | 1818329383031537664 |
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| author | Björn Berglund Ngoc Thi Bich Hoang Maria Tärnberg Ngai Kien Le Maud Nilsson Dung Thi Khanh Khu Olov Svartström Jenny Welander Lennart E. Nilsson Linus Olson Tran Minh Dien Hai Thanh Le Mattias Larsson Håkan Hanberger |
| author_facet | Björn Berglund Ngoc Thi Bich Hoang Maria Tärnberg Ngai Kien Le Maud Nilsson Dung Thi Khanh Khu Olov Svartström Jenny Welander Lennart E. Nilsson Linus Olson Tran Minh Dien Hai Thanh Le Mattias Larsson Håkan Hanberger |
| author_sort | Björn Berglund |
| collection | DOAJ |
| description | Abstract Background Carbapenem-resistant Klebsiella pneumoniae are becoming increasingly common in hospital settings worldwide and are a source of increased morbidity, mortality and health care costs. The global epidemiology of carbapenem-resistant K. pneumoniae is characterized by different strains distributed geographically, with the strain ST258 being predominant in Europe and USA, and ST11 being most common in East Asia. ST15 is a less frequently occurring strain but has nevertheless been reported worldwide as a source of hospital outbreaks of carbapenem-resistant K. pneumoniae. Methods In this study, whole-genome sequencing and antimicrobial susceptibility testing was used to characterize 57 clinical isolates of carbapenem-resistant K. pneumoniae belonging to a strain of ST15, which were collected at a Vietnamese pediatric hospital from February throughout September 2015. Results Aside from the carbapenem resistance gene bla KPC-2, which was carried by all isolates, prevalence of resistance genes to other antibiotics including aminoglycosides, macrolides, quinolones, fosfomycin and trimethoprim, was also high. All isolates were multidrug-resistant. Susceptibility was highest to ceftazidime/avibactam (96%), gentamicin (91%) and tigecycline (82%). Notably, the colistin resistance rate was very high (42%). Single-nucleotide polymorphism analysis indicated that most isolates belonged to a single clone. Conclusions The diverse variety of antibiotic resistance genes and the high antibiotic resistance rates to last-resort antibiotics such as carbapenems and colistin, is indicative of a highly adaptable strain. This emphasizes the importance of implementation of infection controls measures, continued monitoring of antibiotic resistance and prudent use of antibiotics to prevent further selection of resistant strains and the emergence of pan-resistant clones. |
| first_indexed | 2024-12-13T12:47:11Z |
| format | Article |
| id | doaj.art-777d06040ea64939983f180199af213c |
| institution | Directory Open Access Journal |
| issn | 2047-2994 |
| language | English |
| last_indexed | 2024-12-13T12:47:11Z |
| publishDate | 2019-10-01 |
| publisher | BMC |
| record_format | Article |
| series | Antimicrobial Resistance and Infection Control |
| spelling | doaj.art-777d06040ea64939983f180199af213c2022-12-21T23:45:28ZengBMCAntimicrobial Resistance and Infection Control2047-29942019-10-01811810.1186/s13756-019-0613-4Molecular and phenotypic characterization of clinical isolates belonging to a KPC-2-producing strain of ST15 Klebsiella pneumoniae from a Vietnamese pediatric hospitalBjörn Berglund0Ngoc Thi Bich Hoang1Maria Tärnberg2Ngai Kien Le3Maud Nilsson4Dung Thi Khanh Khu5Olov Svartström6Jenny Welander7Lennart E. Nilsson8Linus Olson9Tran Minh Dien10Hai Thanh Le11Mattias Larsson12Håkan Hanberger13Department of Clinical and Experimental Medicine, Linköping UniversityVietnam National Children’s HospitalDepartment of Clinical and Experimental Medicine, Linköping UniversityVietnam National Children’s HospitalDepartment of Clinical and Experimental Medicine, Linköping UniversityVietnam National Children’s HospitalDepartment of Clinical Microbiology and Department of Clinical and Experimental Medicine, Linköping UniversityDepartment of Clinical Microbiology and Department of Clinical and Experimental Medicine, Linköping UniversityDepartment of Clinical and Experimental Medicine, Linköping UniversityTraining and Research Academic Collaboration (TRAC)– Sweden – VietnamVietnam National Children’s HospitalVietnam National Children’s HospitalTraining and Research Academic Collaboration (TRAC)– Sweden – VietnamDepartment of Clinical and Experimental Medicine, Linköping UniversityAbstract Background Carbapenem-resistant Klebsiella pneumoniae are becoming increasingly common in hospital settings worldwide and are a source of increased morbidity, mortality and health care costs. The global epidemiology of carbapenem-resistant K. pneumoniae is characterized by different strains distributed geographically, with the strain ST258 being predominant in Europe and USA, and ST11 being most common in East Asia. ST15 is a less frequently occurring strain but has nevertheless been reported worldwide as a source of hospital outbreaks of carbapenem-resistant K. pneumoniae. Methods In this study, whole-genome sequencing and antimicrobial susceptibility testing was used to characterize 57 clinical isolates of carbapenem-resistant K. pneumoniae belonging to a strain of ST15, which were collected at a Vietnamese pediatric hospital from February throughout September 2015. Results Aside from the carbapenem resistance gene bla KPC-2, which was carried by all isolates, prevalence of resistance genes to other antibiotics including aminoglycosides, macrolides, quinolones, fosfomycin and trimethoprim, was also high. All isolates were multidrug-resistant. Susceptibility was highest to ceftazidime/avibactam (96%), gentamicin (91%) and tigecycline (82%). Notably, the colistin resistance rate was very high (42%). Single-nucleotide polymorphism analysis indicated that most isolates belonged to a single clone. Conclusions The diverse variety of antibiotic resistance genes and the high antibiotic resistance rates to last-resort antibiotics such as carbapenems and colistin, is indicative of a highly adaptable strain. This emphasizes the importance of implementation of infection controls measures, continued monitoring of antibiotic resistance and prudent use of antibiotics to prevent further selection of resistant strains and the emergence of pan-resistant clones.http://link.springer.com/article/10.1186/s13756-019-0613-4Antibiotic resistanceCarbapenemsKlebsiella pneumoniaeVietnamWhole-genome sequencing |
| spellingShingle | Björn Berglund Ngoc Thi Bich Hoang Maria Tärnberg Ngai Kien Le Maud Nilsson Dung Thi Khanh Khu Olov Svartström Jenny Welander Lennart E. Nilsson Linus Olson Tran Minh Dien Hai Thanh Le Mattias Larsson Håkan Hanberger Molecular and phenotypic characterization of clinical isolates belonging to a KPC-2-producing strain of ST15 Klebsiella pneumoniae from a Vietnamese pediatric hospital Antimicrobial Resistance and Infection Control Antibiotic resistance Carbapenems Klebsiella pneumoniae Vietnam Whole-genome sequencing |
| title | Molecular and phenotypic characterization of clinical isolates belonging to a KPC-2-producing strain of ST15 Klebsiella pneumoniae from a Vietnamese pediatric hospital |
| title_full | Molecular and phenotypic characterization of clinical isolates belonging to a KPC-2-producing strain of ST15 Klebsiella pneumoniae from a Vietnamese pediatric hospital |
| title_fullStr | Molecular and phenotypic characterization of clinical isolates belonging to a KPC-2-producing strain of ST15 Klebsiella pneumoniae from a Vietnamese pediatric hospital |
| title_full_unstemmed | Molecular and phenotypic characterization of clinical isolates belonging to a KPC-2-producing strain of ST15 Klebsiella pneumoniae from a Vietnamese pediatric hospital |
| title_short | Molecular and phenotypic characterization of clinical isolates belonging to a KPC-2-producing strain of ST15 Klebsiella pneumoniae from a Vietnamese pediatric hospital |
| title_sort | molecular and phenotypic characterization of clinical isolates belonging to a kpc 2 producing strain of st15 klebsiella pneumoniae from a vietnamese pediatric hospital |
| topic | Antibiotic resistance Carbapenems Klebsiella pneumoniae Vietnam Whole-genome sequencing |
| url | http://link.springer.com/article/10.1186/s13756-019-0613-4 |
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