Screening chemical modulators of benzoic acid derivatives to improve lipid accumulation in Schizochytrium limacinum SR21 with metabolomics analysis
Abstract Background Schizochytrium sp. is a marine fungus with great potential as an alternative commercial source of lipids rich in polyunsaturated fatty acids (PUFAs). To further increase lipid accumulation in Schizochytrium sp., the effect of exogenous additives has become one of the hotspots of...
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| Format: | Article |
| Language: | English |
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BMC
2019-09-01
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| Series: | Biotechnology for Biofuels |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s13068-019-1552-2 |
| _version_ | 1817980280128929792 |
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| author | Zhipeng Li Xueping Ling Hao Zhou Tong Meng Jinjin Zeng Wei Hang Yanyan Shi Ning He |
| author_facet | Zhipeng Li Xueping Ling Hao Zhou Tong Meng Jinjin Zeng Wei Hang Yanyan Shi Ning He |
| author_sort | Zhipeng Li |
| collection | DOAJ |
| description | Abstract Background Schizochytrium sp. is a marine fungus with great potential as an alternative commercial source of lipids rich in polyunsaturated fatty acids (PUFAs). To further increase lipid accumulation in Schizochytrium sp., the effect of exogenous additives has become one of the hotspots of current research. Although benzoic acid derivatives showed positive effects on lipid accumulation in Schizochytrium, the biochemical mechanism needs further investigation. Results Four benzoic acid derivatives (sodium benzoate, p-aminobenzoic acid, p-methyl benzoic acid and folic acid) were screened and evaluated for their effect on lipid accumulation in Schizochytrium limacinum SR21. The lipid yield was increased by 56.84% with p-aminobenzoic acid (p-ABA) at a concentration of 200 mg/L among the four tested chemical modulators. The metabolomics analysis showed that 200 mg/L p-ABA was optimal for promoting glucose catabolism in glycolysis with an increase in the mevalonate pathway and a weakening of the tricarboxylic acid (TCA) cycle. Moreover, p-ABA increased NADPH generation by enhancing the pentose phosphate pathway (PPP), ultimately redirecting the metabolic flux to lipid synthesis. Fed-batch fermentation further proved that p-ABA could significantly increase the yield of lipid by 30.01%, reaching 99.67 g/L, and the lipid content was increased by 35.03%, reaching 71.12%. More importantly, the yields of docosahexaenoic acid (DHA) and eicosapentaenoic acid (EPA) were increased by 33.28% and 42.0%, respectively. Conclusion The addition of p-ABA could promote the synthesis of tetrahydrofolate, enhancing NADPH, which ultimately promoted the flow of carbon flux to lipid synthesis. These findings provide a valuable strategy for improving the lipid accumulation in Schizochytrium by additives. |
| first_indexed | 2024-04-13T22:52:24Z |
| format | Article |
| id | doaj.art-777e4112312c4d9eb1c7a69060c6587b |
| institution | Directory Open Access Journal |
| issn | 1754-6834 |
| language | English |
| last_indexed | 2024-04-13T22:52:24Z |
| publishDate | 2019-09-01 |
| publisher | BMC |
| record_format | Article |
| series | Biotechnology for Biofuels |
| spelling | doaj.art-777e4112312c4d9eb1c7a69060c6587b2022-12-22T02:26:09ZengBMCBiotechnology for Biofuels1754-68342019-09-0112111110.1186/s13068-019-1552-2Screening chemical modulators of benzoic acid derivatives to improve lipid accumulation in Schizochytrium limacinum SR21 with metabolomics analysisZhipeng Li0Xueping Ling1Hao Zhou2Tong Meng3Jinjin Zeng4Wei Hang5Yanyan Shi6Ning He7Department of Chemical and Biochemical Engineering, College of Chemistry and Chemical Engineering, Xiamen UniversityDepartment of Chemical and Biochemical Engineering, College of Chemistry and Chemical Engineering, Xiamen UniversityDepartment of Chemical and Biochemical Engineering, College of Chemistry and Chemical Engineering, Xiamen UniversityDepartment of Chemical and Biochemical Engineering, College of Chemistry and Chemical Engineering, Xiamen UniversityDepartment of Chemical and Biochemical Engineering, College of Chemistry and Chemical Engineering, Xiamen UniversityDepartment of Chemical and Biochemical Engineering, College of Chemistry and Chemical Engineering, Xiamen UniversityDepartment of Chemical and Biochemical Engineering, College of Chemistry and Chemical Engineering, Xiamen UniversityDepartment of Chemical and Biochemical Engineering, College of Chemistry and Chemical Engineering, Xiamen UniversityAbstract Background Schizochytrium sp. is a marine fungus with great potential as an alternative commercial source of lipids rich in polyunsaturated fatty acids (PUFAs). To further increase lipid accumulation in Schizochytrium sp., the effect of exogenous additives has become one of the hotspots of current research. Although benzoic acid derivatives showed positive effects on lipid accumulation in Schizochytrium, the biochemical mechanism needs further investigation. Results Four benzoic acid derivatives (sodium benzoate, p-aminobenzoic acid, p-methyl benzoic acid and folic acid) were screened and evaluated for their effect on lipid accumulation in Schizochytrium limacinum SR21. The lipid yield was increased by 56.84% with p-aminobenzoic acid (p-ABA) at a concentration of 200 mg/L among the four tested chemical modulators. The metabolomics analysis showed that 200 mg/L p-ABA was optimal for promoting glucose catabolism in glycolysis with an increase in the mevalonate pathway and a weakening of the tricarboxylic acid (TCA) cycle. Moreover, p-ABA increased NADPH generation by enhancing the pentose phosphate pathway (PPP), ultimately redirecting the metabolic flux to lipid synthesis. Fed-batch fermentation further proved that p-ABA could significantly increase the yield of lipid by 30.01%, reaching 99.67 g/L, and the lipid content was increased by 35.03%, reaching 71.12%. More importantly, the yields of docosahexaenoic acid (DHA) and eicosapentaenoic acid (EPA) were increased by 33.28% and 42.0%, respectively. Conclusion The addition of p-ABA could promote the synthesis of tetrahydrofolate, enhancing NADPH, which ultimately promoted the flow of carbon flux to lipid synthesis. These findings provide a valuable strategy for improving the lipid accumulation in Schizochytrium by additives.http://link.springer.com/article/10.1186/s13068-019-1552-2SchizochytriumLipidBenzoic acid derivativesMetabolomics |
| spellingShingle | Zhipeng Li Xueping Ling Hao Zhou Tong Meng Jinjin Zeng Wei Hang Yanyan Shi Ning He Screening chemical modulators of benzoic acid derivatives to improve lipid accumulation in Schizochytrium limacinum SR21 with metabolomics analysis Biotechnology for Biofuels Schizochytrium Lipid Benzoic acid derivatives Metabolomics |
| title | Screening chemical modulators of benzoic acid derivatives to improve lipid accumulation in Schizochytrium limacinum SR21 with metabolomics analysis |
| title_full | Screening chemical modulators of benzoic acid derivatives to improve lipid accumulation in Schizochytrium limacinum SR21 with metabolomics analysis |
| title_fullStr | Screening chemical modulators of benzoic acid derivatives to improve lipid accumulation in Schizochytrium limacinum SR21 with metabolomics analysis |
| title_full_unstemmed | Screening chemical modulators of benzoic acid derivatives to improve lipid accumulation in Schizochytrium limacinum SR21 with metabolomics analysis |
| title_short | Screening chemical modulators of benzoic acid derivatives to improve lipid accumulation in Schizochytrium limacinum SR21 with metabolomics analysis |
| title_sort | screening chemical modulators of benzoic acid derivatives to improve lipid accumulation in schizochytrium limacinum sr21 with metabolomics analysis |
| topic | Schizochytrium Lipid Benzoic acid derivatives Metabolomics |
| url | http://link.springer.com/article/10.1186/s13068-019-1552-2 |
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