Comparison of cell viability and morphology of a human osteoblast-like cell line (SaOS-2) seeded on various bone substitute materials: An in vitro study
Background: Many studies have shown favorable results following the use of different bone graft materials. The aim of the present study was to evaluate the biocompatibility of four different bone graft materials regarding cell viability and morphology of Human osteoblast-like cells (SaOS-2) in vitro...
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| Format: | Article |
| Language: | English |
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Wolters Kluwer Medknow Publications
2012-01-01
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| Series: | Dental Research Journal |
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| Online Access: | http://www.drjjournal.net/article.asp?issn=1735-3327;year=2012;volume=9;issue=1;spage=86;epage=92;aulast=Ayobian-Markazi |
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| author | Nader Ayobian-Markazi T Fourootan M J Kharazifar |
| author_facet | Nader Ayobian-Markazi T Fourootan M J Kharazifar |
| author_sort | Nader Ayobian-Markazi |
| collection | DOAJ |
| description | Background: Many studies have shown favorable results following the use of different bone graft materials. The aim of the present study was to evaluate the biocompatibility of four different bone graft materials regarding cell viability and morphology of Human osteoblast-like cells (SaOS-2) in vitro.
Materials and Methods: The effects of Bio-Oss® , Tutodent® , Osteon® , and Cerasorb® were studied on the human osteoblast-like cell line to evaluate various parameters. Human osteoblast-like cells were seeded onto the mentioned bone substitute materials (BSMs). Cell differentiation; cell viability and alkaline phosphatase (ALP) activity of the seeded cells were evaluated by means of scanning electron microscopy, cell viability test and phase contrast microscopy Analysis of variance (ANOVA). Tamhane΄s post-hoc, Kruskal-Wallis Test, and Dunn′s Test were used. The results were considered to be statistically significant at P<0.05.
Results: The control group (SaOS-2 cells which were incubated in Dulbecco Modified Eagle Medium without any kind of bone graft materials) had the highest level of cell viability (P<0.001), followed by Tutodent® , Osteon® , Cerasorb® , and Bio-Oss® . There was no significant difference in MTT assay results between Tutodent® and the control group (P=0.032). All tested bone graft materials showed significantly higher ALP activity than the control (P<0.001). The Tutodent® group showed the best cell growth among all experimental groups, followed by the Osteon® group. The former had a higher spindle-like morphology with good attachment to the surface. Cells cultivated on the surfaces of the Cerasorb® and Bio-Oss® granules had more round morphologies.
Conclusion: This in vitro study demonstrated that all tested BSMs can provide good cell differentiation but a lower rate of proliferation. |
| first_indexed | 2024-12-10T08:25:44Z |
| format | Article |
| id | doaj.art-778014adee364365b5273d4468b41a32 |
| institution | Directory Open Access Journal |
| issn | 1735-3327 2008-0255 |
| language | English |
| last_indexed | 2024-12-10T08:25:44Z |
| publishDate | 2012-01-01 |
| publisher | Wolters Kluwer Medknow Publications |
| record_format | Article |
| series | Dental Research Journal |
| spelling | doaj.art-778014adee364365b5273d4468b41a322022-12-22T01:56:14ZengWolters Kluwer Medknow PublicationsDental Research Journal1735-33272008-02552012-01-0191869210.4103/1735-3327.92959Comparison of cell viability and morphology of a human osteoblast-like cell line (SaOS-2) seeded on various bone substitute materials: An in vitro studyNader Ayobian-MarkaziT FourootanM J KharazifarBackground: Many studies have shown favorable results following the use of different bone graft materials. The aim of the present study was to evaluate the biocompatibility of four different bone graft materials regarding cell viability and morphology of Human osteoblast-like cells (SaOS-2) in vitro. Materials and Methods: The effects of Bio-Oss® , Tutodent® , Osteon® , and Cerasorb® were studied on the human osteoblast-like cell line to evaluate various parameters. Human osteoblast-like cells were seeded onto the mentioned bone substitute materials (BSMs). Cell differentiation; cell viability and alkaline phosphatase (ALP) activity of the seeded cells were evaluated by means of scanning electron microscopy, cell viability test and phase contrast microscopy Analysis of variance (ANOVA). Tamhane΄s post-hoc, Kruskal-Wallis Test, and Dunn′s Test were used. The results were considered to be statistically significant at P<0.05. Results: The control group (SaOS-2 cells which were incubated in Dulbecco Modified Eagle Medium without any kind of bone graft materials) had the highest level of cell viability (P<0.001), followed by Tutodent® , Osteon® , Cerasorb® , and Bio-Oss® . There was no significant difference in MTT assay results between Tutodent® and the control group (P=0.032). All tested bone graft materials showed significantly higher ALP activity than the control (P<0.001). The Tutodent® group showed the best cell growth among all experimental groups, followed by the Osteon® group. The former had a higher spindle-like morphology with good attachment to the surface. Cells cultivated on the surfaces of the Cerasorb® and Bio-Oss® granules had more round morphologies. Conclusion: This in vitro study demonstrated that all tested BSMs can provide good cell differentiation but a lower rate of proliferation.http://www.drjjournal.net/article.asp?issn=1735-3327;year=2012;volume=9;issue=1;spage=86;epage=92;aulast=Ayobian-MarkaziAlkaline phosphatasebone graftcell culturecell morphologycell viabilityosteoblast |
| spellingShingle | Nader Ayobian-Markazi T Fourootan M J Kharazifar Comparison of cell viability and morphology of a human osteoblast-like cell line (SaOS-2) seeded on various bone substitute materials: An in vitro study Dental Research Journal Alkaline phosphatase bone graft cell culture cell morphology cell viability osteoblast |
| title | Comparison of cell viability and morphology of a human osteoblast-like cell line (SaOS-2) seeded on various bone substitute materials: An in vitro study |
| title_full | Comparison of cell viability and morphology of a human osteoblast-like cell line (SaOS-2) seeded on various bone substitute materials: An in vitro study |
| title_fullStr | Comparison of cell viability and morphology of a human osteoblast-like cell line (SaOS-2) seeded on various bone substitute materials: An in vitro study |
| title_full_unstemmed | Comparison of cell viability and morphology of a human osteoblast-like cell line (SaOS-2) seeded on various bone substitute materials: An in vitro study |
| title_short | Comparison of cell viability and morphology of a human osteoblast-like cell line (SaOS-2) seeded on various bone substitute materials: An in vitro study |
| title_sort | comparison of cell viability and morphology of a human osteoblast like cell line saos 2 seeded on various bone substitute materials an in vitro study |
| topic | Alkaline phosphatase bone graft cell culture cell morphology cell viability osteoblast |
| url | http://www.drjjournal.net/article.asp?issn=1735-3327;year=2012;volume=9;issue=1;spage=86;epage=92;aulast=Ayobian-Markazi |
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