Western diet changes cardiac acyl-CoA composition in obese rats: a potential role for hepatic lipogenesis[S]
The “lipotoxic footprint” of cardiac maladaptation in diet-induced obesity is poorly defined. We investigated how manipulation of dietary lipid and carbohydrate influenced potential lipotoxic species in the failing heart. In Wistar rats, contractile dysfunction develops at 48 weeks on a high-fat/hig...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2010-06-01
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| Series: | Journal of Lipid Research |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S0022227520410120 |
| _version_ | 1818601429385871360 |
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| author | Romain Harmancey Christopher R. Wilson Nathan R. Wright Heinrich Taegtmeyer |
| author_facet | Romain Harmancey Christopher R. Wilson Nathan R. Wright Heinrich Taegtmeyer |
| author_sort | Romain Harmancey |
| collection | DOAJ |
| description | The “lipotoxic footprint” of cardiac maladaptation in diet-induced obesity is poorly defined. We investigated how manipulation of dietary lipid and carbohydrate influenced potential lipotoxic species in the failing heart. In Wistar rats, contractile dysfunction develops at 48 weeks on a high-fat/high-carbohydrate “Western” diet, but not on low-fat/high-carbohydrate or high-fat diets. Cardiac content of the lipotoxic candidates—diacylglycerol, ceramide, lipid peroxide, and long-chain acyl-CoA species—was measured at different time points by high-performance liquid chromatography and biochemical assays, as was lipogenic capacity in the heart and liver by qRT-PCR and radiometric assays. Changes in membranes fluidity were also monitored using fluorescence polarization. We report that Western feeding induced a 40% decrease in myocardial palmitoleoyl-CoA content and a similar decrease in the unsaturated-to-saturated fatty acid ratio. These changes were associated with impaired cardiac mitochondrial membrane fluidity. At the same time, hepatic lipogenic capacity was increased in animals fed Western diet (+270% fatty acid elongase activity compared with high-fat diet), while fatty acid desaturase activity decreased over time. Our findings suggest that dysregulation of lipogenesis is a significant component of heart failure in diet-induced obesity. |
| first_indexed | 2024-12-16T12:51:14Z |
| format | Article |
| id | doaj.art-77810b2929574011a22a0dd8a7260c15 |
| institution | Directory Open Access Journal |
| issn | 0022-2275 |
| language | English |
| last_indexed | 2024-12-16T12:51:14Z |
| publishDate | 2010-06-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Lipid Research |
| spelling | doaj.art-77810b2929574011a22a0dd8a7260c152022-12-21T22:31:08ZengElsevierJournal of Lipid Research0022-22752010-06-0151613801393Western diet changes cardiac acyl-CoA composition in obese rats: a potential role for hepatic lipogenesis[S]Romain Harmancey0Christopher R. Wilson1Nathan R. Wright2Heinrich Taegtmeyer3Department of Internal Medicine, Division of Cardiology, University of Texas Medical School at Houston, Houston, TX 77030Department of Internal Medicine, Division of Cardiology, University of Texas Medical School at Houston, Houston, TX 77030Department of Internal Medicine, Division of Cardiology, University of Texas Medical School at Houston, Houston, TX 77030Department of Internal Medicine, Division of Cardiology, University of Texas Medical School at Houston, Houston, TX 77030The “lipotoxic footprint” of cardiac maladaptation in diet-induced obesity is poorly defined. We investigated how manipulation of dietary lipid and carbohydrate influenced potential lipotoxic species in the failing heart. In Wistar rats, contractile dysfunction develops at 48 weeks on a high-fat/high-carbohydrate “Western” diet, but not on low-fat/high-carbohydrate or high-fat diets. Cardiac content of the lipotoxic candidates—diacylglycerol, ceramide, lipid peroxide, and long-chain acyl-CoA species—was measured at different time points by high-performance liquid chromatography and biochemical assays, as was lipogenic capacity in the heart and liver by qRT-PCR and radiometric assays. Changes in membranes fluidity were also monitored using fluorescence polarization. We report that Western feeding induced a 40% decrease in myocardial palmitoleoyl-CoA content and a similar decrease in the unsaturated-to-saturated fatty acid ratio. These changes were associated with impaired cardiac mitochondrial membrane fluidity. At the same time, hepatic lipogenic capacity was increased in animals fed Western diet (+270% fatty acid elongase activity compared with high-fat diet), while fatty acid desaturase activity decreased over time. Our findings suggest that dysregulation of lipogenesis is a significant component of heart failure in diet-induced obesity.http://www.sciencedirect.com/science/article/pii/S0022227520410120heart failurelipotoxicitydyslipidemiametabolic syndromefatty acid |
| spellingShingle | Romain Harmancey Christopher R. Wilson Nathan R. Wright Heinrich Taegtmeyer Western diet changes cardiac acyl-CoA composition in obese rats: a potential role for hepatic lipogenesis[S] Journal of Lipid Research heart failure lipotoxicity dyslipidemia metabolic syndrome fatty acid |
| title | Western diet changes cardiac acyl-CoA composition in obese rats: a potential role for hepatic lipogenesis[S] |
| title_full | Western diet changes cardiac acyl-CoA composition in obese rats: a potential role for hepatic lipogenesis[S] |
| title_fullStr | Western diet changes cardiac acyl-CoA composition in obese rats: a potential role for hepatic lipogenesis[S] |
| title_full_unstemmed | Western diet changes cardiac acyl-CoA composition in obese rats: a potential role for hepatic lipogenesis[S] |
| title_short | Western diet changes cardiac acyl-CoA composition in obese rats: a potential role for hepatic lipogenesis[S] |
| title_sort | western diet changes cardiac acyl coa composition in obese rats a potential role for hepatic lipogenesis s |
| topic | heart failure lipotoxicity dyslipidemia metabolic syndrome fatty acid |
| url | http://www.sciencedirect.com/science/article/pii/S0022227520410120 |
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