Glioblastoma U-87 cell electrotaxis is hindered by doxycycline with a concomitant reduction in the matrix metallopeptidase-9 expression
Electric fields (EF) play an essential role in cancer cell migration. Numerous cancer cell types exhibit electrotaxis under direct current electric fields (dcEF) of physiological electric field strength (EFs). This study investigated the effects of doxycycline on the electrotactic responses of U87 c...
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| Format: | Article |
| Language: | English |
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Elsevier
2024-07-01
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| Series: | Biochemistry and Biophysics Reports |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2405580824000542 |
| _version_ | 1797242955723964416 |
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| author | Hui-Fang Chang Ji-Yen Cheng |
| author_facet | Hui-Fang Chang Ji-Yen Cheng |
| author_sort | Hui-Fang Chang |
| collection | DOAJ |
| description | Electric fields (EF) play an essential role in cancer cell migration. Numerous cancer cell types exhibit electrotaxis under direct current electric fields (dcEF) of physiological electric field strength (EFs). This study investigated the effects of doxycycline on the electrotactic responses of U87 cells. After EF stimulation, U87 cells migrated toward the cathode, whereas doxycycline-treated U87 cells exhibited enhanced cell mobility but hindered cathodal migration. We further investigated the expression of the metastasis-correlated proteins matrix metallopeptidase-2 (MMP-2) and MMP-9 in U87 cells. The levels of MMP-2 in the cells were not altered under EF or doxycycline stimulation. In contrast, the EF stimulation greatly enhanced the levels of MMP-9 and then repressed in doxycycline-cotreated cells, accompanied by reduced cathodal migration. Our results demonstrated that an antibiotic at a non-toxic concentration could suppress the enhanced cell migration accelerated by EF of physiological strength. This finding may be applied as an anti-metastatic treatment for cancers. |
| first_indexed | 2024-04-24T18:47:27Z |
| format | Article |
| id | doaj.art-77831ca5e789475cb1a0a27fb2d82a46 |
| institution | Directory Open Access Journal |
| issn | 2405-5808 |
| language | English |
| last_indexed | 2024-04-24T18:47:27Z |
| publishDate | 2024-07-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Biochemistry and Biophysics Reports |
| spelling | doaj.art-77831ca5e789475cb1a0a27fb2d82a462024-03-27T04:52:22ZengElsevierBiochemistry and Biophysics Reports2405-58082024-07-0138101690Glioblastoma U-87 cell electrotaxis is hindered by doxycycline with a concomitant reduction in the matrix metallopeptidase-9 expressionHui-Fang Chang0Ji-Yen Cheng1Research Center for Applied Sciences, Academia Sinica, Taipei, TaiwanResearch Center for Applied Sciences, Academia Sinica, Taipei, Taiwan; Institute of Biophotonics, National Yang Ming Chiao Tung University, Taipei, Taiwan; Department of Mechanical and Mechatronic Engineering, National Taiwan Ocean University, Keelung, Taiwan; College of Engineering, Chang Gung University, Taoyuan, Taiwan; Corresponding author. Research Center for Applied Sciences, Academia Sinica, No. 128, Sec. 2, Academia Rd., Nangang Dist., Taipei City, 115201, Taiwan.Electric fields (EF) play an essential role in cancer cell migration. Numerous cancer cell types exhibit electrotaxis under direct current electric fields (dcEF) of physiological electric field strength (EFs). This study investigated the effects of doxycycline on the electrotactic responses of U87 cells. After EF stimulation, U87 cells migrated toward the cathode, whereas doxycycline-treated U87 cells exhibited enhanced cell mobility but hindered cathodal migration. We further investigated the expression of the metastasis-correlated proteins matrix metallopeptidase-2 (MMP-2) and MMP-9 in U87 cells. The levels of MMP-2 in the cells were not altered under EF or doxycycline stimulation. In contrast, the EF stimulation greatly enhanced the levels of MMP-9 and then repressed in doxycycline-cotreated cells, accompanied by reduced cathodal migration. Our results demonstrated that an antibiotic at a non-toxic concentration could suppress the enhanced cell migration accelerated by EF of physiological strength. This finding may be applied as an anti-metastatic treatment for cancers.http://www.sciencedirect.com/science/article/pii/S2405580824000542DoxycyclineElectrotaxisMicrofluidicCancer metastasisGlioblastoma cellMMP-9 |
| spellingShingle | Hui-Fang Chang Ji-Yen Cheng Glioblastoma U-87 cell electrotaxis is hindered by doxycycline with a concomitant reduction in the matrix metallopeptidase-9 expression Biochemistry and Biophysics Reports Doxycycline Electrotaxis Microfluidic Cancer metastasis Glioblastoma cell MMP-9 |
| title | Glioblastoma U-87 cell electrotaxis is hindered by doxycycline with a concomitant reduction in the matrix metallopeptidase-9 expression |
| title_full | Glioblastoma U-87 cell electrotaxis is hindered by doxycycline with a concomitant reduction in the matrix metallopeptidase-9 expression |
| title_fullStr | Glioblastoma U-87 cell electrotaxis is hindered by doxycycline with a concomitant reduction in the matrix metallopeptidase-9 expression |
| title_full_unstemmed | Glioblastoma U-87 cell electrotaxis is hindered by doxycycline with a concomitant reduction in the matrix metallopeptidase-9 expression |
| title_short | Glioblastoma U-87 cell electrotaxis is hindered by doxycycline with a concomitant reduction in the matrix metallopeptidase-9 expression |
| title_sort | glioblastoma u 87 cell electrotaxis is hindered by doxycycline with a concomitant reduction in the matrix metallopeptidase 9 expression |
| topic | Doxycycline Electrotaxis Microfluidic Cancer metastasis Glioblastoma cell MMP-9 |
| url | http://www.sciencedirect.com/science/article/pii/S2405580824000542 |
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