Dual Crosslinked Ion-Based Bacterial Cellulose Composite Hydrogel Containing Polyhexamethylene Biguanide
Composite bacterial cellulose (BC) based hydrogel with alginate (A) or pectin (P) or alginate and pectin was fabricated via a physical crosslinking technique using calcium chloride (CaCl<sub>2</sub>) solution and incorporated with polyhexamethylene biguanide (PHMB) as an effective antimi...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-08-01
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| Series: | Membranes |
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| Online Access: | https://www.mdpi.com/2077-0375/12/9/825 |
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| author | Baramee Chanabodeechalermrung Tanpong Chaiwarit Sarana Rose Sommano Pornchai Rachtanapun Nutthapong Kantrong Chuda Chittasupho Pensak Jantrawut |
| author_facet | Baramee Chanabodeechalermrung Tanpong Chaiwarit Sarana Rose Sommano Pornchai Rachtanapun Nutthapong Kantrong Chuda Chittasupho Pensak Jantrawut |
| author_sort | Baramee Chanabodeechalermrung |
| collection | DOAJ |
| description | Composite bacterial cellulose (BC) based hydrogel with alginate (A) or pectin (P) or alginate and pectin was fabricated via a physical crosslinking technique using calcium chloride (CaCl<sub>2</sub>) solution and incorporated with polyhexamethylene biguanide (PHMB) as an effective antimicrobial drug by immersion method. After that, the physicochemical properties of all hydrogel formulations were characterized. The result showed that the formulations with PHMB performed better physicochemical properties than the hydrogel without PHMB. Fourier transform infrared spectroscopy (FT-IR) showed the interaction between PHMB and the carboxylic group of alginate and pectin. BC/A-PHMB hydrogel performed suitable mechanical strength, fluid uptake ability, water retention property, drug content, high integrity value, and maximum swelling degree. Moreover, in vitro cell viability of BC/A-PHMB hydrogel revealed high biocompatibility with human keratinocyte cell line (HaCaT) and demonstrated prolong released of PHMB in Tris-HCl buffer pH 7.4, while rapid release in phosphate buffer saline pH 7.4. BC/A-PHMB hydrogel demonstrated good anti-bacterial activity against <i>S. aureus</i> and <i>P. aeruginosa</i>. In conclusion, BC/A-PHMB hydrogel could be a potential dual crosslinked ion-based hydrogel for wound dressing with anti-bacterial activity. |
| first_indexed | 2024-03-09T23:12:43Z |
| format | Article |
| id | doaj.art-7783e11a5349488c82557e1eba796520 |
| institution | Directory Open Access Journal |
| issn | 2077-0375 |
| language | English |
| last_indexed | 2024-03-09T23:12:43Z |
| publishDate | 2022-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Membranes |
| spelling | doaj.art-7783e11a5349488c82557e1eba7965202023-11-23T17:42:00ZengMDPI AGMembranes2077-03752022-08-0112982510.3390/membranes12090825Dual Crosslinked Ion-Based Bacterial Cellulose Composite Hydrogel Containing Polyhexamethylene BiguanideBaramee Chanabodeechalermrung0Tanpong Chaiwarit1Sarana Rose Sommano2Pornchai Rachtanapun3Nutthapong Kantrong4Chuda Chittasupho5Pensak Jantrawut6Department of Pharmaceutical Sciences, Faculty of Pharmacy, Chiang Mai University, Chiang Mai 50200, ThailandDepartment of Pharmaceutical Sciences, Faculty of Pharmacy, Chiang Mai University, Chiang Mai 50200, ThailandPlant Bioactive Compound Laboratory (BAC), Department of Plant and Soil Sciences, Faculty of Agriculture, Chiang Mai University, Chiang Mai 50200, ThailandCluster of Research and Development of Pharmaceutical and Natural Products Innovation for Human or Animal, Chiang Mai University, Chiang Mai 50200, ThailandDepartment of Restorative Dentistry, Faculty of Dentistry, Khon Kaen University, Khon Kaen 40002, ThailandDepartment of Pharmaceutical Sciences, Faculty of Pharmacy, Chiang Mai University, Chiang Mai 50200, ThailandDepartment of Pharmaceutical Sciences, Faculty of Pharmacy, Chiang Mai University, Chiang Mai 50200, ThailandComposite bacterial cellulose (BC) based hydrogel with alginate (A) or pectin (P) or alginate and pectin was fabricated via a physical crosslinking technique using calcium chloride (CaCl<sub>2</sub>) solution and incorporated with polyhexamethylene biguanide (PHMB) as an effective antimicrobial drug by immersion method. After that, the physicochemical properties of all hydrogel formulations were characterized. The result showed that the formulations with PHMB performed better physicochemical properties than the hydrogel without PHMB. Fourier transform infrared spectroscopy (FT-IR) showed the interaction between PHMB and the carboxylic group of alginate and pectin. BC/A-PHMB hydrogel performed suitable mechanical strength, fluid uptake ability, water retention property, drug content, high integrity value, and maximum swelling degree. Moreover, in vitro cell viability of BC/A-PHMB hydrogel revealed high biocompatibility with human keratinocyte cell line (HaCaT) and demonstrated prolong released of PHMB in Tris-HCl buffer pH 7.4, while rapid release in phosphate buffer saline pH 7.4. BC/A-PHMB hydrogel demonstrated good anti-bacterial activity against <i>S. aureus</i> and <i>P. aeruginosa</i>. In conclusion, BC/A-PHMB hydrogel could be a potential dual crosslinked ion-based hydrogel for wound dressing with anti-bacterial activity.https://www.mdpi.com/2077-0375/12/9/825bacterial cellulosecrosslinkinghydrogelalginatepectin |
| spellingShingle | Baramee Chanabodeechalermrung Tanpong Chaiwarit Sarana Rose Sommano Pornchai Rachtanapun Nutthapong Kantrong Chuda Chittasupho Pensak Jantrawut Dual Crosslinked Ion-Based Bacterial Cellulose Composite Hydrogel Containing Polyhexamethylene Biguanide Membranes bacterial cellulose crosslinking hydrogel alginate pectin |
| title | Dual Crosslinked Ion-Based Bacterial Cellulose Composite Hydrogel Containing Polyhexamethylene Biguanide |
| title_full | Dual Crosslinked Ion-Based Bacterial Cellulose Composite Hydrogel Containing Polyhexamethylene Biguanide |
| title_fullStr | Dual Crosslinked Ion-Based Bacterial Cellulose Composite Hydrogel Containing Polyhexamethylene Biguanide |
| title_full_unstemmed | Dual Crosslinked Ion-Based Bacterial Cellulose Composite Hydrogel Containing Polyhexamethylene Biguanide |
| title_short | Dual Crosslinked Ion-Based Bacterial Cellulose Composite Hydrogel Containing Polyhexamethylene Biguanide |
| title_sort | dual crosslinked ion based bacterial cellulose composite hydrogel containing polyhexamethylene biguanide |
| topic | bacterial cellulose crosslinking hydrogel alginate pectin |
| url | https://www.mdpi.com/2077-0375/12/9/825 |
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