Longitudinal Study of Fecal Microbiota in Calves with or without Diarrhea Episodes before Weaning
The microbiota plays an important role in the development of diarrhea in pre-weaned calves. The characterization of the fecal microbiota in health and disease can be critical to unravel the bacterial dynamics associated with diarrhea and help with its prevention and control. In this study, we aimed...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-08-01
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| Series: | Veterinary Sciences |
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| Online Access: | https://www.mdpi.com/2306-7381/9/9/463 |
| _version_ | 1797481490147180544 |
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| author | Pau Obregon-Gutierrez Jaume Bague-Companys Alex Bach Virginia Aragon Florencia Correa-Fiz |
| author_facet | Pau Obregon-Gutierrez Jaume Bague-Companys Alex Bach Virginia Aragon Florencia Correa-Fiz |
| author_sort | Pau Obregon-Gutierrez |
| collection | DOAJ |
| description | The microbiota plays an important role in the development of diarrhea in pre-weaned calves. The characterization of the fecal microbiota in health and disease can be critical to unravel the bacterial dynamics associated with diarrhea and help with its prevention and control. In this study, we aimed to detect changes in the fecal microbiota of calves that experienced early-life diarrhea episodes. Fecal samples were taken from calves remaining healthy and calves with an episode of diarrhea during the study. We sampled at arrival (12 days of age) and after one and two months of life; also, at the time of the diarrhea episode for the diarrheic calves (day 17). Samples were processed to extract total DNA, submitted to 16S rRNA gene sequencing, and bioinformatically analyzed to infer the bacterial populations. Microbiota changes through time were reported for both groups. However, we detected an earlier stabilization in the healthy group. Moreover, we detected changes within low abundant taxa that may play a role in the subsequent health status of the animals. The fecal microbiota of healthy and diarrheic calves showed different dynamics in the diversity through time that may be the reflections of the variations within low-abundant taxa. |
| first_indexed | 2024-03-09T22:15:23Z |
| format | Article |
| id | doaj.art-7786c56143604b37a59c9edfca3a88fa |
| institution | Directory Open Access Journal |
| issn | 2306-7381 |
| language | English |
| last_indexed | 2024-03-09T22:15:23Z |
| publishDate | 2022-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Veterinary Sciences |
| spelling | doaj.art-7786c56143604b37a59c9edfca3a88fa2023-11-23T19:23:48ZengMDPI AGVeterinary Sciences2306-73812022-08-019946310.3390/vetsci9090463Longitudinal Study of Fecal Microbiota in Calves with or without Diarrhea Episodes before WeaningPau Obregon-Gutierrez0Jaume Bague-Companys1Alex Bach2Virginia Aragon3Florencia Correa-Fiz4Unitat Mixta d’Investigació IRTA-UAB en Sanitat Animal, Centre de Recerca en Sanitat Animal (CReSA), Campus de la Universitat Autònoma de Barcelona (UAB), Bellaterra, 08193 Barcelona, SpainUnitat Mixta d’Investigació IRTA-UAB en Sanitat Animal, Centre de Recerca en Sanitat Animal (CReSA), Campus de la Universitat Autònoma de Barcelona (UAB), Bellaterra, 08193 Barcelona, SpainMarlex Recerca i Educació, 08173 Barcelona, SpainUnitat Mixta d’Investigació IRTA-UAB en Sanitat Animal, Centre de Recerca en Sanitat Animal (CReSA), Campus de la Universitat Autònoma de Barcelona (UAB), Bellaterra, 08193 Barcelona, SpainUnitat Mixta d’Investigació IRTA-UAB en Sanitat Animal, Centre de Recerca en Sanitat Animal (CReSA), Campus de la Universitat Autònoma de Barcelona (UAB), Bellaterra, 08193 Barcelona, SpainThe microbiota plays an important role in the development of diarrhea in pre-weaned calves. The characterization of the fecal microbiota in health and disease can be critical to unravel the bacterial dynamics associated with diarrhea and help with its prevention and control. In this study, we aimed to detect changes in the fecal microbiota of calves that experienced early-life diarrhea episodes. Fecal samples were taken from calves remaining healthy and calves with an episode of diarrhea during the study. We sampled at arrival (12 days of age) and after one and two months of life; also, at the time of the diarrhea episode for the diarrheic calves (day 17). Samples were processed to extract total DNA, submitted to 16S rRNA gene sequencing, and bioinformatically analyzed to infer the bacterial populations. Microbiota changes through time were reported for both groups. However, we detected an earlier stabilization in the healthy group. Moreover, we detected changes within low abundant taxa that may play a role in the subsequent health status of the animals. The fecal microbiota of healthy and diarrheic calves showed different dynamics in the diversity through time that may be the reflections of the variations within low-abundant taxa.https://www.mdpi.com/2306-7381/9/9/463fecal microbiotadiversity dynamicscalfcattlediarrhea |
| spellingShingle | Pau Obregon-Gutierrez Jaume Bague-Companys Alex Bach Virginia Aragon Florencia Correa-Fiz Longitudinal Study of Fecal Microbiota in Calves with or without Diarrhea Episodes before Weaning Veterinary Sciences fecal microbiota diversity dynamics calf cattle diarrhea |
| title | Longitudinal Study of Fecal Microbiota in Calves with or without Diarrhea Episodes before Weaning |
| title_full | Longitudinal Study of Fecal Microbiota in Calves with or without Diarrhea Episodes before Weaning |
| title_fullStr | Longitudinal Study of Fecal Microbiota in Calves with or without Diarrhea Episodes before Weaning |
| title_full_unstemmed | Longitudinal Study of Fecal Microbiota in Calves with or without Diarrhea Episodes before Weaning |
| title_short | Longitudinal Study of Fecal Microbiota in Calves with or without Diarrhea Episodes before Weaning |
| title_sort | longitudinal study of fecal microbiota in calves with or without diarrhea episodes before weaning |
| topic | fecal microbiota diversity dynamics calf cattle diarrhea |
| url | https://www.mdpi.com/2306-7381/9/9/463 |
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