COMPARATIVE STUDY OF DIFFERENT VACCINATION ROUTES AGAINST NEWCASTLE DISEASE IN LAYER CHICKENS
In the present study, a trail to evaluate of Newcastle Disease (ND) antibodies levels after different vaccination programs was conducted on layer chickens. A total of 200 one day-old layer chicks (White Lohmann) were divided into five groups A, B, C, D and E. Birds in groups A, B and C were vaccinat...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | Arabic |
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The Union of Arab Universities
2019-03-01
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| Series: | Arab Universities Journal of Agricultural Sciences |
| Subjects: | |
| Online Access: | https://ajs.journals.ekb.eg/article_43354_4d89a8291db498ae997ab50870a5fbd6.pdf |
| _version_ | 1827323591671152640 |
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| author | Samar El-Masry M. Nasr-Eldin Abeer Faiesal B. Othman |
| author_facet | Samar El-Masry M. Nasr-Eldin Abeer Faiesal B. Othman |
| author_sort | Samar El-Masry |
| collection | DOAJ |
| description | In the present study, a trail to evaluate of Newcastle Disease (ND) antibodies levels after different vaccination programs was conducted on layer chickens. A total of 200 one day-old layer chicks (White Lohmann) were divided into five groups A, B, C, D and E. Birds in groups A, B and C were vaccinated with live vaccine by intraocular, intranasal and drinking water methods , respectively. On the other hand, groups D and E were kept as unvaccinated control groups. Vaccination performed at days 5, 18 and 28 by different routes for mentioned groups. Hemagglutination inhibition (HI) and enzyme-linked immunosorbent assay (ELISA) methods were used for assessment of antibodies titer at days 15, 25, 37 and 45. Results of HI and ELISA tests indicated that, the intranasal and the intraocular method have highest antibodies titers compared with the drinking water method. In this study, maternally derived antibodies specific to Newcastle Disease Virus (NDV) (IgY) were extracted by dextran sulfate method from collected eggs of vaccinated laying chickens . Antibodies specific to NDV (IgY) were detected in the egg yolk using HI test . Data revealed that antibodies specific to NDV (IgY) were presented in high titers that confer protection during early weeks of life for hatching chicks. Data concluded that extraction of maternally derived specific antibodies from egg yolk will facilitate accurate monitoring of ND vaccination programmes. |
| first_indexed | 2024-04-25T01:54:16Z |
| format | Article |
| id | doaj.art-778dba8912b34f32941ffeb58cade38a |
| institution | Directory Open Access Journal |
| issn | 1110-2675 2636-3585 |
| language | Arabic |
| last_indexed | 2024-04-25T01:54:16Z |
| publishDate | 2019-03-01 |
| publisher | The Union of Arab Universities |
| record_format | Article |
| series | Arab Universities Journal of Agricultural Sciences |
| spelling | doaj.art-778dba8912b34f32941ffeb58cade38a2024-03-07T17:37:41ZaraThe Union of Arab UniversitiesArab Universities Journal of Agricultural Sciences1110-26752636-35852019-03-0127123123810.21608/ajs.2019.4335443354COMPARATIVE STUDY OF DIFFERENT VACCINATION ROUTES AGAINST NEWCASTLE DISEASE IN LAYER CHICKENSSamar El-Masry0M. Nasr-Eldin1Abeer Faiesal2B. Othman3Agric. Microbiology Dept., Fac. of Agric., Ain Shams Univ., P.O. Box 68, Hadayek Shobra 11241, Cairo, EgyptBotany Dept., Fac. of Science, Benha Univ., Kalyobya, Egypt.Basic and Applied Agric. Sciences Dept., Higher Institute for Agric. Co-Operation, Cairo, EgyptAgric. Microbiology Dept., Fac. of Agric., Ain Shams Univ., P.O. Box 68, Hadayek Shobra 11241, Cairo, EgyptIn the present study, a trail to evaluate of Newcastle Disease (ND) antibodies levels after different vaccination programs was conducted on layer chickens. A total of 200 one day-old layer chicks (White Lohmann) were divided into five groups A, B, C, D and E. Birds in groups A, B and C were vaccinated with live vaccine by intraocular, intranasal and drinking water methods , respectively. On the other hand, groups D and E were kept as unvaccinated control groups. Vaccination performed at days 5, 18 and 28 by different routes for mentioned groups. Hemagglutination inhibition (HI) and enzyme-linked immunosorbent assay (ELISA) methods were used for assessment of antibodies titer at days 15, 25, 37 and 45. Results of HI and ELISA tests indicated that, the intranasal and the intraocular method have highest antibodies titers compared with the drinking water method. In this study, maternally derived antibodies specific to Newcastle Disease Virus (NDV) (IgY) were extracted by dextran sulfate method from collected eggs of vaccinated laying chickens . Antibodies specific to NDV (IgY) were detected in the egg yolk using HI test . Data revealed that antibodies specific to NDV (IgY) were presented in high titers that confer protection during early weeks of life for hatching chicks. Data concluded that extraction of maternally derived specific antibodies from egg yolk will facilitate accurate monitoring of ND vaccination programmes.https://ajs.journals.ekb.eg/article_43354_4d89a8291db498ae997ab50870a5fbd6.pdfvaccination routesndlayer chickensantibody titerelisahi test |
| spellingShingle | Samar El-Masry M. Nasr-Eldin Abeer Faiesal B. Othman COMPARATIVE STUDY OF DIFFERENT VACCINATION ROUTES AGAINST NEWCASTLE DISEASE IN LAYER CHICKENS Arab Universities Journal of Agricultural Sciences vaccination routes nd layer chickens antibody titer elisa hi test |
| title | COMPARATIVE STUDY OF DIFFERENT VACCINATION ROUTES AGAINST NEWCASTLE DISEASE IN LAYER CHICKENS |
| title_full | COMPARATIVE STUDY OF DIFFERENT VACCINATION ROUTES AGAINST NEWCASTLE DISEASE IN LAYER CHICKENS |
| title_fullStr | COMPARATIVE STUDY OF DIFFERENT VACCINATION ROUTES AGAINST NEWCASTLE DISEASE IN LAYER CHICKENS |
| title_full_unstemmed | COMPARATIVE STUDY OF DIFFERENT VACCINATION ROUTES AGAINST NEWCASTLE DISEASE IN LAYER CHICKENS |
| title_short | COMPARATIVE STUDY OF DIFFERENT VACCINATION ROUTES AGAINST NEWCASTLE DISEASE IN LAYER CHICKENS |
| title_sort | comparative study of different vaccination routes against newcastle disease in layer chickens |
| topic | vaccination routes nd layer chickens antibody titer elisa hi test |
| url | https://ajs.journals.ekb.eg/article_43354_4d89a8291db498ae997ab50870a5fbd6.pdf |
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