Bacterial Inhibition and Osteogenic Potentials of Sr/Zn Co-Doped Nano-Hydroxyapatite-PLGA Composite Scaffold for Bone Tissue Engineering Applications
Bacterial infection associated with bone grafts is one of the major challenges that can lead to implant failure. Treatment of these infections is a costly endeavor; therefore, an ideal bone scaffold should merge both biocompatibility and antibacterial activity. Antibiotic-impregnated scaffolds may p...
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| Format: | Article |
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MDPI AG
2023-03-01
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| Series: | Polymers |
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| Online Access: | https://www.mdpi.com/2073-4360/15/6/1370 |
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| author | Mozan Hassan Abbas Khaleel Sherif Mohamed Karam Ali Hassan Al-Marzouqi Ihtesham ur Rehman Sahar Mohsin |
| author_facet | Mozan Hassan Abbas Khaleel Sherif Mohamed Karam Ali Hassan Al-Marzouqi Ihtesham ur Rehman Sahar Mohsin |
| author_sort | Mozan Hassan |
| collection | DOAJ |
| description | Bacterial infection associated with bone grafts is one of the major challenges that can lead to implant failure. Treatment of these infections is a costly endeavor; therefore, an ideal bone scaffold should merge both biocompatibility and antibacterial activity. Antibiotic-impregnated scaffolds may prevent bacterial colonization but exacerbate the global antibiotic resistance problem. Recent approaches combined scaffolds with metal ions that have antimicrobial properties. In our study, a unique strontium/zinc (Sr/Zn) co-doped nanohydroxyapatite (nHAp) and Poly (lactic-co-glycolic acid) -(PLGA) composite scaffold was fabricated using a chemical precipitation method with different ratios of Sr/Zn ions (1%, 2.5%, and 4%). The scaffolds’ antibacterial activity against <i>Staphylococcus aureus</i> were evaluated by counting bacterial colony-forming unit (CFU) numbers after direct contact with the scaffolds. The results showed a dose-dependent reduction in CFU numbers as the Zn concentration increased, with 4% Zn showing the best antibacterial properties of all the Zn-containing scaffolds. PLGA incorporation in Sr/Zn-nHAp did not affect the Zn antibacterial activity and the 4% Sr/Zn-nHAp-PLGA scaffold showed a 99.7% bacterial growth inhibition. MTT (3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) cell viability assay showed that Sr/Zn co-doping supported osteoblast cell proliferation with no apparent cytotoxicity and the highest doping percentage in the 4% Sr/Zn-nHAp-PLGA was found to be ideal for cell growth. In conclusion, these findings demonstrate the potential for a 4% Sr/Zn-nHAp-PLGA scaffold with enhanced antibacterial activity and cytocompatibility as a suitable candidate for bone regeneration. |
| first_indexed | 2024-03-11T06:00:17Z |
| format | Article |
| id | doaj.art-7791c4d7852a4bec87bf93d74447ccb2 |
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| issn | 2073-4360 |
| language | English |
| last_indexed | 2024-03-11T06:00:17Z |
| publishDate | 2023-03-01 |
| publisher | MDPI AG |
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| series | Polymers |
| spelling | doaj.art-7791c4d7852a4bec87bf93d74447ccb22023-11-17T13:24:36ZengMDPI AGPolymers2073-43602023-03-01156137010.3390/polym15061370Bacterial Inhibition and Osteogenic Potentials of Sr/Zn Co-Doped Nano-Hydroxyapatite-PLGA Composite Scaffold for Bone Tissue Engineering ApplicationsMozan Hassan0Abbas Khaleel1Sherif Mohamed Karam2Ali Hassan Al-Marzouqi3Ihtesham ur Rehman4Sahar Mohsin5Department of Anatomy, College of Medicine and Health Sciences, United Arab Emirates University, Al Ain P.O. Box 15551, United Arab EmiratesDepartment of Chemistry, College of Science, United Arab Emirates University, Al Ain P.O. Box 15551, United Arab EmiratesDepartment of Anatomy, College of Medicine and Health Sciences, United Arab Emirates University, Al Ain P.O. Box 15551, United Arab EmiratesDepartment of Chemical and Petroleum Engineering, College of Engineering, United Arab Emirates University, Al Ain P.O. Box 15551, United Arab EmiratesSchool of Medicine, University of Central Lancashire, Preston PR1 2HE, UKDepartment of Anatomy, College of Medicine and Health Sciences, United Arab Emirates University, Al Ain P.O. Box 15551, United Arab EmiratesBacterial infection associated with bone grafts is one of the major challenges that can lead to implant failure. Treatment of these infections is a costly endeavor; therefore, an ideal bone scaffold should merge both biocompatibility and antibacterial activity. Antibiotic-impregnated scaffolds may prevent bacterial colonization but exacerbate the global antibiotic resistance problem. Recent approaches combined scaffolds with metal ions that have antimicrobial properties. In our study, a unique strontium/zinc (Sr/Zn) co-doped nanohydroxyapatite (nHAp) and Poly (lactic-co-glycolic acid) -(PLGA) composite scaffold was fabricated using a chemical precipitation method with different ratios of Sr/Zn ions (1%, 2.5%, and 4%). The scaffolds’ antibacterial activity against <i>Staphylococcus aureus</i> were evaluated by counting bacterial colony-forming unit (CFU) numbers after direct contact with the scaffolds. The results showed a dose-dependent reduction in CFU numbers as the Zn concentration increased, with 4% Zn showing the best antibacterial properties of all the Zn-containing scaffolds. PLGA incorporation in Sr/Zn-nHAp did not affect the Zn antibacterial activity and the 4% Sr/Zn-nHAp-PLGA scaffold showed a 99.7% bacterial growth inhibition. MTT (3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) cell viability assay showed that Sr/Zn co-doping supported osteoblast cell proliferation with no apparent cytotoxicity and the highest doping percentage in the 4% Sr/Zn-nHAp-PLGA was found to be ideal for cell growth. In conclusion, these findings demonstrate the potential for a 4% Sr/Zn-nHAp-PLGA scaffold with enhanced antibacterial activity and cytocompatibility as a suitable candidate for bone regeneration.https://www.mdpi.com/2073-4360/15/6/1370strontiumzincnano-hydroxyapatitePLGAantibacterialbone scaffolds |
| spellingShingle | Mozan Hassan Abbas Khaleel Sherif Mohamed Karam Ali Hassan Al-Marzouqi Ihtesham ur Rehman Sahar Mohsin Bacterial Inhibition and Osteogenic Potentials of Sr/Zn Co-Doped Nano-Hydroxyapatite-PLGA Composite Scaffold for Bone Tissue Engineering Applications Polymers strontium zinc nano-hydroxyapatite PLGA antibacterial bone scaffolds |
| title | Bacterial Inhibition and Osteogenic Potentials of Sr/Zn Co-Doped Nano-Hydroxyapatite-PLGA Composite Scaffold for Bone Tissue Engineering Applications |
| title_full | Bacterial Inhibition and Osteogenic Potentials of Sr/Zn Co-Doped Nano-Hydroxyapatite-PLGA Composite Scaffold for Bone Tissue Engineering Applications |
| title_fullStr | Bacterial Inhibition and Osteogenic Potentials of Sr/Zn Co-Doped Nano-Hydroxyapatite-PLGA Composite Scaffold for Bone Tissue Engineering Applications |
| title_full_unstemmed | Bacterial Inhibition and Osteogenic Potentials of Sr/Zn Co-Doped Nano-Hydroxyapatite-PLGA Composite Scaffold for Bone Tissue Engineering Applications |
| title_short | Bacterial Inhibition and Osteogenic Potentials of Sr/Zn Co-Doped Nano-Hydroxyapatite-PLGA Composite Scaffold for Bone Tissue Engineering Applications |
| title_sort | bacterial inhibition and osteogenic potentials of sr zn co doped nano hydroxyapatite plga composite scaffold for bone tissue engineering applications |
| topic | strontium zinc nano-hydroxyapatite PLGA antibacterial bone scaffolds |
| url | https://www.mdpi.com/2073-4360/15/6/1370 |
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