High-throughput tuning of ovarian cancer spheroids for on-chip invasion assays
We developed an invasion assay by using microfabricated culture devices. First, ovarian tumor spheroids were generated with a culture patch device consisting of an agarose membrane formed with a honeycomb microframe – the patch – and gelatin nanofiber backbone. By changing the dimensions of the hone...
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| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2022-06-01
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| Series: | Micro and Nano Engineering |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2590007222000351 |
| _version_ | 1817980363266326528 |
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| author | Changchong Chen Yong He Elliot Lopez Franck Carreiras Ayako Yamada Marie-Claire Schanne-Klein Ambroise Lambert Yong Chen Carole Aimé |
| author_facet | Changchong Chen Yong He Elliot Lopez Franck Carreiras Ayako Yamada Marie-Claire Schanne-Klein Ambroise Lambert Yong Chen Carole Aimé |
| author_sort | Changchong Chen |
| collection | DOAJ |
| description | We developed an invasion assay by using microfabricated culture devices. First, ovarian tumor spheroids were generated with a culture patch device consisting of an agarose membrane formed with a honeycomb microframe – the patch – and gelatin nanofiber backbone. By changing the dimensions of the honeycomb compartments we were able to control the number of cells and size of the spheroids. When the spheroids were placed on a patch coated with a thin membrane of fibrillary type I collagen, spheroid disruption was observed due to substrate induced cell migration. This process is straightforward and should be applicable to other cancer types, as well as assays under microfluidic conditions, thereby holding the potential for use in tumor modeling and anti-cancer drug development. |
| first_indexed | 2024-04-13T22:53:02Z |
| format | Article |
| id | doaj.art-77922b6d2e6349f9be46c823a005f64d |
| institution | Directory Open Access Journal |
| issn | 2590-0072 |
| language | English |
| last_indexed | 2024-04-13T22:53:02Z |
| publishDate | 2022-06-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Micro and Nano Engineering |
| spelling | doaj.art-77922b6d2e6349f9be46c823a005f64d2022-12-22T02:26:06ZengElsevierMicro and Nano Engineering2590-00722022-06-0115100138High-throughput tuning of ovarian cancer spheroids for on-chip invasion assaysChangchong Chen0Yong He1Elliot Lopez2Franck Carreiras3Ayako Yamada4Marie-Claire Schanne-Klein5Ambroise Lambert6Yong Chen7Carole Aimé8PASTEUR, Département de chimie, École normale supérieure, PSL University, Sorbonne Université, CNRS, 75005 Paris, FrancePASTEUR, Département de chimie, École normale supérieure, PSL University, Sorbonne Université, CNRS, 75005 Paris, FrancePASTEUR, Département de chimie, École normale supérieure, PSL University, Sorbonne Université, CNRS, 75005 Paris, FranceEquipe de Recherche sur les Relations Matrice Extracellulaire-Cellules, ERRMECe, (EA1391), Groupe Matrice Extracellulaire et physiopathologie (MECuP), Institut des Materiaux, I-MAT (FD4122), CY Cergy Paris Université, FrancePASTEUR, Département de chimie, École normale supérieure, PSL University, Sorbonne Université, CNRS, 75005 Paris, FranceLaboratoire d'Optique et Biosciences, École polytechnique, CNRS, Inserm, Institut Polytechnique de Paris, F-91128 Palaiseau, FranceEquipe de Recherche sur les Relations Matrice Extracellulaire-Cellules, ERRMECe, (EA1391), Groupe Matrice Extracellulaire et physiopathologie (MECuP), Institut des Materiaux, I-MAT (FD4122), CY Cergy Paris Université, FrancePASTEUR, Département de chimie, École normale supérieure, PSL University, Sorbonne Université, CNRS, 75005 Paris, France; Corresponding authors.PASTEUR, Département de chimie, École normale supérieure, PSL University, Sorbonne Université, CNRS, 75005 Paris, France; Corresponding authors.We developed an invasion assay by using microfabricated culture devices. First, ovarian tumor spheroids were generated with a culture patch device consisting of an agarose membrane formed with a honeycomb microframe – the patch – and gelatin nanofiber backbone. By changing the dimensions of the honeycomb compartments we were able to control the number of cells and size of the spheroids. When the spheroids were placed on a patch coated with a thin membrane of fibrillary type I collagen, spheroid disruption was observed due to substrate induced cell migration. This process is straightforward and should be applicable to other cancer types, as well as assays under microfluidic conditions, thereby holding the potential for use in tumor modeling and anti-cancer drug development.http://www.sciencedirect.com/science/article/pii/S2590007222000351Tumor spheroidEpithelial-to-mesenchymal transitionCulture substrateMicrofluidics |
| spellingShingle | Changchong Chen Yong He Elliot Lopez Franck Carreiras Ayako Yamada Marie-Claire Schanne-Klein Ambroise Lambert Yong Chen Carole Aimé High-throughput tuning of ovarian cancer spheroids for on-chip invasion assays Micro and Nano Engineering Tumor spheroid Epithelial-to-mesenchymal transition Culture substrate Microfluidics |
| title | High-throughput tuning of ovarian cancer spheroids for on-chip invasion assays |
| title_full | High-throughput tuning of ovarian cancer spheroids for on-chip invasion assays |
| title_fullStr | High-throughput tuning of ovarian cancer spheroids for on-chip invasion assays |
| title_full_unstemmed | High-throughput tuning of ovarian cancer spheroids for on-chip invasion assays |
| title_short | High-throughput tuning of ovarian cancer spheroids for on-chip invasion assays |
| title_sort | high throughput tuning of ovarian cancer spheroids for on chip invasion assays |
| topic | Tumor spheroid Epithelial-to-mesenchymal transition Culture substrate Microfluidics |
| url | http://www.sciencedirect.com/science/article/pii/S2590007222000351 |
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