Ameliorative effects of supranutritional selenium on TLR‐4‐NF‐kB‐TNF‐α‐mediated hepatic oxidative injury and inflammation in goats fed high concentrate diet
Abstract We examined whether surplus dietary selenium (Se) supply could alleviate high concentrate (HC) diet‐induced hepatic oxidative stress (OS) and inflammation. Eighteen young goats were distributed into three groups; were fed low (LC, concentrate: forage; 35: 65), high concentrate (HC, 65: 35),...
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| Format: | Article |
| Language: | English |
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Wiley
2022-11-01
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| Series: | Food Science & Nutrition |
| Subjects: | |
| Online Access: | https://doi.org/10.1002/fsn3.2980 |
| _version_ | 1797894464105086976 |
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| author | Tahmina Shah Moolchand Malhi Allah Bux Kachiwal Bachal Bhutto Qurban Ali Shah Yan Lei Saeed Ahmed Soomro Jamila Soomro Nazeer Hussain Kalhoro Hongbing Gui |
| author_facet | Tahmina Shah Moolchand Malhi Allah Bux Kachiwal Bachal Bhutto Qurban Ali Shah Yan Lei Saeed Ahmed Soomro Jamila Soomro Nazeer Hussain Kalhoro Hongbing Gui |
| author_sort | Tahmina Shah |
| collection | DOAJ |
| description | Abstract We examined whether surplus dietary selenium (Se) supply could alleviate high concentrate (HC) diet‐induced hepatic oxidative stress (OS) and inflammation. Eighteen young goats were distributed into three groups; were fed low (LC, concentrate: forage; 35: 65), high concentrate (HC, 65: 35), or Se‐supplemented HC (HCSe, 65: 35 + 0.5 mg Se kg−1 diet) diets for 10 weeks. Short chain fatty acids, OS markers and immunoinflammatory genes expressions were assessed through gas chromatograph, kits, and RT‐qPCR, respectively. Compared with LC, HC diet increased (p < .05) colonic and serum lipopolysaccharide (LPS) levels and induced hepatic oxidative injury by increasing (p < .05) malondialdehyde (MDA) levels and decreasing (p < .05) activities of glutathione peroxidase, superoxide dismutase, and catalase. HC diet altered hepatic mRNA expressions of toll‐like receptor‐4 (TLR‐4), cluster of differentiation‐14 (CD‐14), tumor necrosis factor‐α (TNF‐α), TNF receptor‐associated factor‐6 (TRAF‐6), nuclear factor kappa B (NF‐κB), interleukin‐1β (IL‐1β), IL‐10, IL‐13, LPS‐binding protein (LBP), serum amyloid A (SAA), α‐acid glycoprotein (AGP), and albumin (ALB). Conversely, extra‐Se supply lowered LPS and attenuated antioxidant status and inflammation in liver. In conclusion, HC diet induced oxidative lesions and TLR‐4 pathway‐mediated inflammation, whereas supranutritional Se alleviated oxidative and inflammatory lesions through TLR‐4 pathway regulation in goat liver. |
| first_indexed | 2024-04-10T07:10:45Z |
| format | Article |
| id | doaj.art-7792495688d042769cba9f426be165d0 |
| institution | Directory Open Access Journal |
| issn | 2048-7177 |
| language | English |
| last_indexed | 2024-04-10T07:10:45Z |
| publishDate | 2022-11-01 |
| publisher | Wiley |
| record_format | Article |
| series | Food Science & Nutrition |
| spelling | doaj.art-7792495688d042769cba9f426be165d02023-02-26T13:10:39ZengWileyFood Science & Nutrition2048-71772022-11-0110113842385410.1002/fsn3.2980Ameliorative effects of supranutritional selenium on TLR‐4‐NF‐kB‐TNF‐α‐mediated hepatic oxidative injury and inflammation in goats fed high concentrate dietTahmina Shah0Moolchand Malhi1Allah Bux Kachiwal2Bachal Bhutto3Qurban Ali Shah4Yan Lei5Saeed Ahmed Soomro6Jamila Soomro7Nazeer Hussain Kalhoro8Hongbing Gui9Department Veterinary Physiology and Biochemistry Sindh Agricultural University Tandojam PakistanDepartment Veterinary Physiology and Biochemistry Sindh Agricultural University Tandojam PakistanDepartment Veterinary Physiology and Biochemistry Sindh Agricultural University Tandojam PakistanDepartment of Veterinary Parasitology Sindh Agricultural University Tandojam PakistanDepartment Veterinary Pathology Lasbela University of Agriculture, Water and Marine Science Uthal Balochistan PakistanDairy Herd Improvement Center Henan Animal Husbandry Bureau Zhengzhou ChinaDepartment Veterinary Physiology and Biochemistry Sindh Agricultural University Tandojam PakistanDepartment Veterinary Physiology and Biochemistry Sindh Agricultural University Tandojam PakistanSindh Institute of Animal Health Karachi PakistanInstitute of Animal Sciences Jiangsu Academy of Agriculture Science Nanjing ChinaAbstract We examined whether surplus dietary selenium (Se) supply could alleviate high concentrate (HC) diet‐induced hepatic oxidative stress (OS) and inflammation. Eighteen young goats were distributed into three groups; were fed low (LC, concentrate: forage; 35: 65), high concentrate (HC, 65: 35), or Se‐supplemented HC (HCSe, 65: 35 + 0.5 mg Se kg−1 diet) diets for 10 weeks. Short chain fatty acids, OS markers and immunoinflammatory genes expressions were assessed through gas chromatograph, kits, and RT‐qPCR, respectively. Compared with LC, HC diet increased (p < .05) colonic and serum lipopolysaccharide (LPS) levels and induced hepatic oxidative injury by increasing (p < .05) malondialdehyde (MDA) levels and decreasing (p < .05) activities of glutathione peroxidase, superoxide dismutase, and catalase. HC diet altered hepatic mRNA expressions of toll‐like receptor‐4 (TLR‐4), cluster of differentiation‐14 (CD‐14), tumor necrosis factor‐α (TNF‐α), TNF receptor‐associated factor‐6 (TRAF‐6), nuclear factor kappa B (NF‐κB), interleukin‐1β (IL‐1β), IL‐10, IL‐13, LPS‐binding protein (LBP), serum amyloid A (SAA), α‐acid glycoprotein (AGP), and albumin (ALB). Conversely, extra‐Se supply lowered LPS and attenuated antioxidant status and inflammation in liver. In conclusion, HC diet induced oxidative lesions and TLR‐4 pathway‐mediated inflammation, whereas supranutritional Se alleviated oxidative and inflammatory lesions through TLR‐4 pathway regulation in goat liver.https://doi.org/10.1002/fsn3.2980acute phase responsecytokinesinterleukinslipopolysaccharidesoxidative stress |
| spellingShingle | Tahmina Shah Moolchand Malhi Allah Bux Kachiwal Bachal Bhutto Qurban Ali Shah Yan Lei Saeed Ahmed Soomro Jamila Soomro Nazeer Hussain Kalhoro Hongbing Gui Ameliorative effects of supranutritional selenium on TLR‐4‐NF‐kB‐TNF‐α‐mediated hepatic oxidative injury and inflammation in goats fed high concentrate diet Food Science & Nutrition acute phase response cytokines interleukins lipopolysaccharides oxidative stress |
| title | Ameliorative effects of supranutritional selenium on TLR‐4‐NF‐kB‐TNF‐α‐mediated hepatic oxidative injury and inflammation in goats fed high concentrate diet |
| title_full | Ameliorative effects of supranutritional selenium on TLR‐4‐NF‐kB‐TNF‐α‐mediated hepatic oxidative injury and inflammation in goats fed high concentrate diet |
| title_fullStr | Ameliorative effects of supranutritional selenium on TLR‐4‐NF‐kB‐TNF‐α‐mediated hepatic oxidative injury and inflammation in goats fed high concentrate diet |
| title_full_unstemmed | Ameliorative effects of supranutritional selenium on TLR‐4‐NF‐kB‐TNF‐α‐mediated hepatic oxidative injury and inflammation in goats fed high concentrate diet |
| title_short | Ameliorative effects of supranutritional selenium on TLR‐4‐NF‐kB‐TNF‐α‐mediated hepatic oxidative injury and inflammation in goats fed high concentrate diet |
| title_sort | ameliorative effects of supranutritional selenium on tlr 4 nf kb tnf α mediated hepatic oxidative injury and inflammation in goats fed high concentrate diet |
| topic | acute phase response cytokines interleukins lipopolysaccharides oxidative stress |
| url | https://doi.org/10.1002/fsn3.2980 |
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