Detection of Mycobacterium bovis in bovine and bubaline tissues using nested-PCR for TbD1.

Detection of Mycobacterium bovis in bovine and bubaline tissues using nested-PCR for TbD1.

In the present study, a nested-PCR system, targeting the TbD1 region, involving the performance of conventional PCR followed by real-time PCR, was developed to detect Mycobacterium bovis in bovine/bubaline tissue homogenates. The sensitivity and specificity of the reactions were assessed with DNA sa...

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Main Authors: Cristina P Araújo, Ana Luiza A R Osório, Kláudia S G Jorge, Carlos Alberto N Ramos, Antonio Francisco S Filho, Carlos Eugênio S Vidal, Eliana Roxo, Christiane Nishibe, Nalvo F Almeida, Antônio A F Júnior, Marcio R Silva, José Diomedes B Neto, Valíria D Cerqueira, Martín J Zumárraga, Flábio R Araújo
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2014-01-01
Series:PLoS ONE
Online Access:http://europepmc.org/articles/PMC3949733?pdf=render

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