Sulforaphane inhibits hepatic steatosis in rats by down-regulating DGAT2 and ACAT1 expression
Objective To study the effects of sulforaphane (SFN) on the deposition of lipid droplets and the expression of their peripheral proteins in the liver and elucidate the mechanism by which SFN inhibits non-alcoholic fatty liver caused by high-fat diet. Methods Sixty male Wistar rats were randomized in...
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| Format: | Article |
| Language: | zho |
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Editorial Office of Journal of Third Military Medical University
2019-06-01
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| Series: | Di-san junyi daxue xuebao |
| Subjects: | |
| Online Access: | http://aammt.tmmu.edu.cn/Upload/rhtml/201812039.htm |
| _version_ | 1819103288964939776 |
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| author | TIAN Sicong LEI Peng SUN Yao |
| author_facet | TIAN Sicong LEI Peng SUN Yao |
| author_sort | TIAN Sicong |
| collection | DOAJ |
| description | Objective To study the effects of sulforaphane (SFN) on the deposition of lipid droplets and the expression of their peripheral proteins in the liver and elucidate the mechanism by which SFN inhibits non-alcoholic fatty liver caused by high-fat diet. Methods Sixty male Wistar rats were randomized into 6 equal groups (n=10), includinganormal food group, ahigh-fat diet group, 3 high-fat diet groups treated with high (20 mg/kg), moderate (10 mg/kg) and low (5 mg/kg) doses of SFN, andahigh-fat diet group treated withapositive drug (30 mg/kg). Triglyceride and total cholesterol contents were examined and the deposition of lipid droplets in the liver was detected with oil redOstaining in all the rats. The protein expression of DGAT2 and ACAT1 (the key synthases of triglyceride and cholesterol, respectively) in the liver tissues were detected with Western blotting; immunohistochemistry and real-time PCR were performed to examine the expression of the PAT proteins (PLIN1, PLIN2, PLIN3 and PLIN5) at both the protein and mRNA levels in the liver. Results SFN treatment obviously reduced high-fat diet-induced bodyweight gain in the rats, lowered the levels of triglyceride and total cholesterol, lessened lipid droplet deposition in the liver and reduced the volume of the lipid droplets. Treatment with SFN at all the 3 doses significantly down-regulated the expression of ACAT1 and DGAT2 in the liver of the rats (P < 0.01). SFN at the high doses significantly down-regulated the expression of PLIN2 and PLIN5 in liver tissue at both the mRNA and protein levels (P < 0.01, P < 0.05). Conclusion SFN can inhibit the formation of lipid droplets by reducing the expressions of DGAT2, ACAT1, PLIN2 and PLIN5 in the liver of rats. |
| first_indexed | 2024-12-22T01:48:05Z |
| format | Article |
| id | doaj.art-779a2b09aafb4fb9a5ee7c838f54e018 |
| institution | Directory Open Access Journal |
| issn | 1000-5404 |
| language | zho |
| last_indexed | 2024-12-22T01:48:05Z |
| publishDate | 2019-06-01 |
| publisher | Editorial Office of Journal of Third Military Medical University |
| record_format | Article |
| series | Di-san junyi daxue xuebao |
| spelling | doaj.art-779a2b09aafb4fb9a5ee7c838f54e0182022-12-21T18:43:00ZzhoEditorial Office of Journal of Third Military Medical UniversityDi-san junyi daxue xuebao1000-54042019-06-0141121102110910.16016/j.1000-5404.201812039Sulforaphane inhibits hepatic steatosis in rats by down-regulating DGAT2 and ACAT1 expressionTIAN Sicong0LEI Peng1SUN Yao2Faculty of Food Science and Engineering, School of Chemistry and Chemical Engineering, Harbin Institute of Technology, Harbin, Heilongjiang Province, 150001Faculty of Food Science and Engineering, School of Chemistry and Chemical Engineering, Harbin Institute of Technology, Harbin, Heilongjiang Province, 150001Faculty of Food Science and Engineering, School of Chemistry and Chemical Engineering, Harbin Institute of Technology, Harbin, Heilongjiang Province, 150001Objective To study the effects of sulforaphane (SFN) on the deposition of lipid droplets and the expression of their peripheral proteins in the liver and elucidate the mechanism by which SFN inhibits non-alcoholic fatty liver caused by high-fat diet. Methods Sixty male Wistar rats were randomized into 6 equal groups (n=10), includinganormal food group, ahigh-fat diet group, 3 high-fat diet groups treated with high (20 mg/kg), moderate (10 mg/kg) and low (5 mg/kg) doses of SFN, andahigh-fat diet group treated withapositive drug (30 mg/kg). Triglyceride and total cholesterol contents were examined and the deposition of lipid droplets in the liver was detected with oil redOstaining in all the rats. The protein expression of DGAT2 and ACAT1 (the key synthases of triglyceride and cholesterol, respectively) in the liver tissues were detected with Western blotting; immunohistochemistry and real-time PCR were performed to examine the expression of the PAT proteins (PLIN1, PLIN2, PLIN3 and PLIN5) at both the protein and mRNA levels in the liver. Results SFN treatment obviously reduced high-fat diet-induced bodyweight gain in the rats, lowered the levels of triglyceride and total cholesterol, lessened lipid droplet deposition in the liver and reduced the volume of the lipid droplets. Treatment with SFN at all the 3 doses significantly down-regulated the expression of ACAT1 and DGAT2 in the liver of the rats (P < 0.01). SFN at the high doses significantly down-regulated the expression of PLIN2 and PLIN5 in liver tissue at both the mRNA and protein levels (P < 0.01, P < 0.05). Conclusion SFN can inhibit the formation of lipid droplets by reducing the expressions of DGAT2, ACAT1, PLIN2 and PLIN5 in the liver of rats.http://aammt.tmmu.edu.cn/Upload/rhtml/201812039.htmsulforaphanetriglyceridecholesterollipid dropletsdgat2acat1plin2plin5 |
| spellingShingle | TIAN Sicong LEI Peng SUN Yao Sulforaphane inhibits hepatic steatosis in rats by down-regulating DGAT2 and ACAT1 expression Di-san junyi daxue xuebao sulforaphane triglyceride cholesterol lipid droplets dgat2 acat1 plin2 plin5 |
| title | Sulforaphane inhibits hepatic steatosis in rats by down-regulating DGAT2 and ACAT1 expression |
| title_full | Sulforaphane inhibits hepatic steatosis in rats by down-regulating DGAT2 and ACAT1 expression |
| title_fullStr | Sulforaphane inhibits hepatic steatosis in rats by down-regulating DGAT2 and ACAT1 expression |
| title_full_unstemmed | Sulforaphane inhibits hepatic steatosis in rats by down-regulating DGAT2 and ACAT1 expression |
| title_short | Sulforaphane inhibits hepatic steatosis in rats by down-regulating DGAT2 and ACAT1 expression |
| title_sort | sulforaphane inhibits hepatic steatosis in rats by down regulating dgat2 and acat1 expression |
| topic | sulforaphane triglyceride cholesterol lipid droplets dgat2 acat1 plin2 plin5 |
| url | http://aammt.tmmu.edu.cn/Upload/rhtml/201812039.htm |
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