Towards Microorganism-Based Biofuel Cells: The Viability of <i>Saccharomyces</i> <i>cerevisiae</i> Modified by Multiwalled Carbon Nanotubes
This research aimed to evaluate the toxic effect of multi-walled carbon nanotubes (MW-CNTs) on yeast cells in order to apply MW-CNTs for possible improvement of the efficiency of microbial biofuel cells. The SEM and XRD analysis suggested that here used MW-CNTs are in the range of 10–25 nm in diamet...
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MDPI AG
2020-05-01
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| Series: | Nanomaterials |
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| Online Access: | https://www.mdpi.com/2079-4991/10/5/954 |
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| author | Ingrida Bruzaite Juste Rozene Inga Morkvenaite-Vilkonciene Arunas Ramanavicius |
| author_facet | Ingrida Bruzaite Juste Rozene Inga Morkvenaite-Vilkonciene Arunas Ramanavicius |
| author_sort | Ingrida Bruzaite |
| collection | DOAJ |
| description | This research aimed to evaluate the toxic effect of multi-walled carbon nanotubes (MW-CNTs) on yeast cells in order to apply MW-CNTs for possible improvement of the efficiency of microbial biofuel cells. The SEM and XRD analysis suggested that here used MW-CNTs are in the range of 10–25 nm in diameter and their structure was confirmed by Raman spectroscopy. In this study, we evaluated the viability of the yeast <i>Saccharomyces cerevisiae</i> cells, affected by MW-CNTs, by cell count, culture optical density and atomic force microscopy. The yeast cells were exposed towards MW-CNTs (of 2, 50, 100 μg/mL concentrations in water-based solution) for 24 h. A mathematical model was applied for the evaluation of relative growth and relative death rates of yeast cells. We calculated that both of the rates are two times higher in the case if yeasts were treated by 50, 100 μg/mL of MW-CNTs containing solution, comparing to that treated by 0 and 2 μg/mL c of MW-CNTs containing solution. It was determined that the MW-CNTs have some observable effect upon the incubation of the yeast cells. The viability of yeast has decreased together with MW-CNTs concentration only after 5 h of the treatment. Therefore, we predict that the MW-CNTs can be applied for the modification of yeast cells in order to improve electrical charge transfer through the yeast cell membrane and/or the cell wall. |
| first_indexed | 2024-03-10T19:46:42Z |
| format | Article |
| id | doaj.art-779c01eecb254e48bd81da86ea61a068 |
| institution | Directory Open Access Journal |
| issn | 2079-4991 |
| language | English |
| last_indexed | 2024-03-10T19:46:42Z |
| publishDate | 2020-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Nanomaterials |
| spelling | doaj.art-779c01eecb254e48bd81da86ea61a0682023-11-20T00:46:44ZengMDPI AGNanomaterials2079-49912020-05-0110595410.3390/nano10050954Towards Microorganism-Based Biofuel Cells: The Viability of <i>Saccharomyces</i> <i>cerevisiae</i> Modified by Multiwalled Carbon NanotubesIngrida Bruzaite0Juste Rozene1Inga Morkvenaite-Vilkonciene2Arunas Ramanavicius3Department of Chemistry and Bioengineering, Faculty of Fundamental Sciences, Vilnius Gediminas Technical University, 10223 Vilnius, LithuaniaDepartment of Mechatronics, Robotics, and Digital Manufacturing, Faculty of Mechanics, Vilnius Gediminas Technical University, 03109 Vilnius, LithuaniaDepartment of Mechatronics, Robotics, and Digital Manufacturing, Faculty of Mechanics, Vilnius Gediminas Technical University, 03109 Vilnius, LithuaniaDepartment of Physical Chemistry, Faculty of Chemistry and Geosciences, Vilnius University, 03225 Vilnius, LithuaniaThis research aimed to evaluate the toxic effect of multi-walled carbon nanotubes (MW-CNTs) on yeast cells in order to apply MW-CNTs for possible improvement of the efficiency of microbial biofuel cells. The SEM and XRD analysis suggested that here used MW-CNTs are in the range of 10–25 nm in diameter and their structure was confirmed by Raman spectroscopy. In this study, we evaluated the viability of the yeast <i>Saccharomyces cerevisiae</i> cells, affected by MW-CNTs, by cell count, culture optical density and atomic force microscopy. The yeast cells were exposed towards MW-CNTs (of 2, 50, 100 μg/mL concentrations in water-based solution) for 24 h. A mathematical model was applied for the evaluation of relative growth and relative death rates of yeast cells. We calculated that both of the rates are two times higher in the case if yeasts were treated by 50, 100 μg/mL of MW-CNTs containing solution, comparing to that treated by 0 and 2 μg/mL c of MW-CNTs containing solution. It was determined that the MW-CNTs have some observable effect upon the incubation of the yeast cells. The viability of yeast has decreased together with MW-CNTs concentration only after 5 h of the treatment. Therefore, we predict that the MW-CNTs can be applied for the modification of yeast cells in order to improve electrical charge transfer through the yeast cell membrane and/or the cell wall.https://www.mdpi.com/2079-4991/10/5/954biocompatibilitycell viabilitycarbon nanotubes<i>Saccharomyces cerevisiae</i>X-ray diffraction |
| spellingShingle | Ingrida Bruzaite Juste Rozene Inga Morkvenaite-Vilkonciene Arunas Ramanavicius Towards Microorganism-Based Biofuel Cells: The Viability of <i>Saccharomyces</i> <i>cerevisiae</i> Modified by Multiwalled Carbon Nanotubes Nanomaterials biocompatibility cell viability carbon nanotubes <i>Saccharomyces cerevisiae</i> X-ray diffraction |
| title | Towards Microorganism-Based Biofuel Cells: The Viability of <i>Saccharomyces</i> <i>cerevisiae</i> Modified by Multiwalled Carbon Nanotubes |
| title_full | Towards Microorganism-Based Biofuel Cells: The Viability of <i>Saccharomyces</i> <i>cerevisiae</i> Modified by Multiwalled Carbon Nanotubes |
| title_fullStr | Towards Microorganism-Based Biofuel Cells: The Viability of <i>Saccharomyces</i> <i>cerevisiae</i> Modified by Multiwalled Carbon Nanotubes |
| title_full_unstemmed | Towards Microorganism-Based Biofuel Cells: The Viability of <i>Saccharomyces</i> <i>cerevisiae</i> Modified by Multiwalled Carbon Nanotubes |
| title_short | Towards Microorganism-Based Biofuel Cells: The Viability of <i>Saccharomyces</i> <i>cerevisiae</i> Modified by Multiwalled Carbon Nanotubes |
| title_sort | towards microorganism based biofuel cells the viability of i saccharomyces i i cerevisiae i modified by multiwalled carbon nanotubes |
| topic | biocompatibility cell viability carbon nanotubes <i>Saccharomyces cerevisiae</i> X-ray diffraction |
| url | https://www.mdpi.com/2079-4991/10/5/954 |
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