The Impact of Exercise Serum on Selected Parameters of CD4+ T Cell Metabolism
CD4+ T cells are sensitive to peripheral changes of cytokine levels and metabolic substrates such as glucose and lactate. This study aimed to analyze whether factors released after exercise alter parameters of human T cell metabolism, specifically glycolysis and oxidative phosphorylation. We used pr...
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-06-01
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| Series: | Immuno |
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| Online Access: | https://www.mdpi.com/2673-5601/1/3/8 |
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| author | Jana Palmowski Kristina Gebhardt Thomas Reichel Torsten Frech Robert Ringseis Klaus Eder Kathrin Renner-Sattler Karsten Krüger |
| author_facet | Jana Palmowski Kristina Gebhardt Thomas Reichel Torsten Frech Robert Ringseis Klaus Eder Kathrin Renner-Sattler Karsten Krüger |
| author_sort | Jana Palmowski |
| collection | DOAJ |
| description | CD4+ T cells are sensitive to peripheral changes of cytokine levels and metabolic substrates such as glucose and lactate. This study aimed to analyze whether factors released after exercise alter parameters of human T cell metabolism, specifically glycolysis and oxidative phosphorylation. We used primary human CD4+ T cells activated in the presence of autologous serum, which was collected before (CO) and after a 30-min exercise intervention (EX). In the course of activation, cells and supernatants were analyzed for cell viability and diameter, real-time oxygen consumption by using PreSens Technology, mRNA expression of glycolytic enzymes and complexes of the electron transport chain by real-time PCR, glucose, and lactate levels in supernatants, and in vitro differentiation by flow cytometry. EX did not alter T cell phenotype, viability, or on-blast formation. Similarly, no difference between CO and EX were found for CD4+ T cell activation and cellular oxygen consumption. In contrast, higher levels of glucose were found after 48 h activation in EX conditions. T cells activated in autologous exercise serum expressed lower HK1 mRNA and higher IFN-γ receptor 1. We suggest that the exercise protocol used was not sufficient to destabilize the immune metabolism of T cells. Therefore, more intense and prolonged exercise should be used in future studies. |
| first_indexed | 2024-03-10T07:34:54Z |
| format | Article |
| id | doaj.art-779f59cc197d457084652263dd7395bd |
| institution | Directory Open Access Journal |
| issn | 2673-5601 |
| language | English |
| last_indexed | 2024-03-10T07:34:54Z |
| publishDate | 2021-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Immuno |
| spelling | doaj.art-779f59cc197d457084652263dd7395bd2023-11-22T13:34:29ZengMDPI AGImmuno2673-56012021-06-011311913110.3390/immuno1030008The Impact of Exercise Serum on Selected Parameters of CD4+ T Cell MetabolismJana Palmowski0Kristina Gebhardt1Thomas Reichel2Torsten Frech3Robert Ringseis4Klaus Eder5Kathrin Renner-Sattler6Karsten Krüger7Department of Exercise Physiology and Sports Therapy, Institute of Sports Science, Justus-Liebig University Giessen, 35394 Giessen, GermanyDepartment of Exercise Physiology and Sports Therapy, Institute of Sports Science, Justus-Liebig University Giessen, 35394 Giessen, GermanyDepartment of Exercise Physiology and Sports Therapy, Institute of Sports Science, Justus-Liebig University Giessen, 35394 Giessen, GermanyDepartment of Exercise Physiology and Sports Therapy, Institute of Sports Science, Justus-Liebig University Giessen, 35394 Giessen, GermanyInstitute of Animal Nutrition and Nutrition Physiology, Justus-Liebig University Giessen, 35392 Giessen, GermanyInstitute of Animal Nutrition and Nutrition Physiology, Justus-Liebig University Giessen, 35392 Giessen, GermanyRegensburg Center for Interventional Immunology (RCI), 93053 Regensburg, GermanyDepartment of Exercise Physiology and Sports Therapy, Institute of Sports Science, Justus-Liebig University Giessen, 35394 Giessen, GermanyCD4+ T cells are sensitive to peripheral changes of cytokine levels and metabolic substrates such as glucose and lactate. This study aimed to analyze whether factors released after exercise alter parameters of human T cell metabolism, specifically glycolysis and oxidative phosphorylation. We used primary human CD4+ T cells activated in the presence of autologous serum, which was collected before (CO) and after a 30-min exercise intervention (EX). In the course of activation, cells and supernatants were analyzed for cell viability and diameter, real-time oxygen consumption by using PreSens Technology, mRNA expression of glycolytic enzymes and complexes of the electron transport chain by real-time PCR, glucose, and lactate levels in supernatants, and in vitro differentiation by flow cytometry. EX did not alter T cell phenotype, viability, or on-blast formation. Similarly, no difference between CO and EX were found for CD4+ T cell activation and cellular oxygen consumption. In contrast, higher levels of glucose were found after 48 h activation in EX conditions. T cells activated in autologous exercise serum expressed lower HK1 mRNA and higher IFN-γ receptor 1. We suggest that the exercise protocol used was not sufficient to destabilize the immune metabolism of T cells. Therefore, more intense and prolonged exercise should be used in future studies.https://www.mdpi.com/2673-5601/1/3/8energy metabolismT cellexercise boutglycolysisoxidative phosphorylationT cell receptor |
| spellingShingle | Jana Palmowski Kristina Gebhardt Thomas Reichel Torsten Frech Robert Ringseis Klaus Eder Kathrin Renner-Sattler Karsten Krüger The Impact of Exercise Serum on Selected Parameters of CD4+ T Cell Metabolism Immuno energy metabolism T cell exercise bout glycolysis oxidative phosphorylation T cell receptor |
| title | The Impact of Exercise Serum on Selected Parameters of CD4+ T Cell Metabolism |
| title_full | The Impact of Exercise Serum on Selected Parameters of CD4+ T Cell Metabolism |
| title_fullStr | The Impact of Exercise Serum on Selected Parameters of CD4+ T Cell Metabolism |
| title_full_unstemmed | The Impact of Exercise Serum on Selected Parameters of CD4+ T Cell Metabolism |
| title_short | The Impact of Exercise Serum on Selected Parameters of CD4+ T Cell Metabolism |
| title_sort | impact of exercise serum on selected parameters of cd4 t cell metabolism |
| topic | energy metabolism T cell exercise bout glycolysis oxidative phosphorylation T cell receptor |
| url | https://www.mdpi.com/2673-5601/1/3/8 |
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