Upregulation of a novel eukaryotic translation initiation factor 5A (eIF5A) in dengue 2 virus-infected mosquito cells
<p>Abstract</p> <p>Background</p> <p>Dengue virus, a mosquito-borne flavivirus, is the etiological agent of dengue fever, dengue hemorrhagic fever, and dengue shock syndrome. It generally induces apoptosis in mammalian cells, but frequently results in persistent infecti...
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| Format: | Article |
| Language: | English |
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BMC
2010-09-01
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| Series: | Virology Journal |
| Online Access: | http://www.virologyj.com/content/7/1/214 |
| _version_ | 1818327479112171520 |
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| author | Shih Yu-Tzu Yang Chao-Fu Chen Wei-June |
| author_facet | Shih Yu-Tzu Yang Chao-Fu Chen Wei-June |
| author_sort | Shih Yu-Tzu |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Background</p> <p>Dengue virus, a mosquito-borne flavivirus, is the etiological agent of dengue fever, dengue hemorrhagic fever, and dengue shock syndrome. It generally induces apoptosis in mammalian cells, but frequently results in persistent infection in mosquito cells. That mechanism remains to be explored. In turn, a genomic survey through subtractive hybridization (PCR-select cDNA subtraction) was conducted in order to find gene(s) that may play a role in interactions between the virus and its host cells.</p> <p>Results</p> <p>Through this technique, we identified a novel eukaryotic translation initiation factor 5A (eIF5A) which is upregulated in <it>Aedes albopictus</it>-derived C6/36 cells infected by the type 2 dengue (Den-2) virus. The full-length of the identified eIF5A gene consisted of 1498 bp of nucleotides with a 41.39% G+C content, and it possessed a higher similarity and shorter evolutionary distance with insects than with other organisms. Upregulation of eIF5A in response to Den-2 virus infection was validated at both the RNA and protein levels. This phenomenon was also observed by confocal microscopy. In addition, cell death obviously occurred when eIF5A activity was inhibited in C6/36 cells even when they were infected by the virus. However, viral multiplication was not obviously affected in infected C6/36 cells when eIF5A activity was reduced.</p> <p>Conclusions</p> <p>Taken together, we postulated that eIF5A plays a role in preventing mosquito cells from death in response to Den-2 viral infection, thus facilitating continued viral growth and potential persistent infection in mosquito cells. It would be worthwhile to further investigate how its downstream factors or cofactors contribute to this phenomenon of dengue infection.</p> |
| first_indexed | 2024-12-13T12:16:55Z |
| format | Article |
| id | doaj.art-77a17dea3cfc471e8abb6612636e1334 |
| institution | Directory Open Access Journal |
| issn | 1743-422X |
| language | English |
| last_indexed | 2024-12-13T12:16:55Z |
| publishDate | 2010-09-01 |
| publisher | BMC |
| record_format | Article |
| series | Virology Journal |
| spelling | doaj.art-77a17dea3cfc471e8abb6612636e13342022-12-21T23:46:42ZengBMCVirology Journal1743-422X2010-09-017121410.1186/1743-422X-7-214Upregulation of a novel eukaryotic translation initiation factor 5A (eIF5A) in dengue 2 virus-infected mosquito cellsShih Yu-TzuYang Chao-FuChen Wei-June<p>Abstract</p> <p>Background</p> <p>Dengue virus, a mosquito-borne flavivirus, is the etiological agent of dengue fever, dengue hemorrhagic fever, and dengue shock syndrome. It generally induces apoptosis in mammalian cells, but frequently results in persistent infection in mosquito cells. That mechanism remains to be explored. In turn, a genomic survey through subtractive hybridization (PCR-select cDNA subtraction) was conducted in order to find gene(s) that may play a role in interactions between the virus and its host cells.</p> <p>Results</p> <p>Through this technique, we identified a novel eukaryotic translation initiation factor 5A (eIF5A) which is upregulated in <it>Aedes albopictus</it>-derived C6/36 cells infected by the type 2 dengue (Den-2) virus. The full-length of the identified eIF5A gene consisted of 1498 bp of nucleotides with a 41.39% G+C content, and it possessed a higher similarity and shorter evolutionary distance with insects than with other organisms. Upregulation of eIF5A in response to Den-2 virus infection was validated at both the RNA and protein levels. This phenomenon was also observed by confocal microscopy. In addition, cell death obviously occurred when eIF5A activity was inhibited in C6/36 cells even when they were infected by the virus. However, viral multiplication was not obviously affected in infected C6/36 cells when eIF5A activity was reduced.</p> <p>Conclusions</p> <p>Taken together, we postulated that eIF5A plays a role in preventing mosquito cells from death in response to Den-2 viral infection, thus facilitating continued viral growth and potential persistent infection in mosquito cells. It would be worthwhile to further investigate how its downstream factors or cofactors contribute to this phenomenon of dengue infection.</p>http://www.virologyj.com/content/7/1/214 |
| spellingShingle | Shih Yu-Tzu Yang Chao-Fu Chen Wei-June Upregulation of a novel eukaryotic translation initiation factor 5A (eIF5A) in dengue 2 virus-infected mosquito cells Virology Journal |
| title | Upregulation of a novel eukaryotic translation initiation factor 5A (eIF5A) in dengue 2 virus-infected mosquito cells |
| title_full | Upregulation of a novel eukaryotic translation initiation factor 5A (eIF5A) in dengue 2 virus-infected mosquito cells |
| title_fullStr | Upregulation of a novel eukaryotic translation initiation factor 5A (eIF5A) in dengue 2 virus-infected mosquito cells |
| title_full_unstemmed | Upregulation of a novel eukaryotic translation initiation factor 5A (eIF5A) in dengue 2 virus-infected mosquito cells |
| title_short | Upregulation of a novel eukaryotic translation initiation factor 5A (eIF5A) in dengue 2 virus-infected mosquito cells |
| title_sort | upregulation of a novel eukaryotic translation initiation factor 5a eif5a in dengue 2 virus infected mosquito cells |
| url | http://www.virologyj.com/content/7/1/214 |
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