Molecular identification of Ralstonia pseudosolanacearum causing bacterial wilt in tomatoes from Da Nang by using Colony PCR
Ralstonia solanacearum species complex (RSSC) is the main pathogen causing bacterial wilt disease in tomatoes. This study applied the colony polymerase chain reaction (PCR) technique to rapidly screen and select RSSC strains from isolated bacteria of diseased tomatoes. This method directly used bac...
| Main Authors: | , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
CAN THO UNIVERSITY PUBLISHING HOUSE
2023-03-01
|
| Series: | Can Tho University Journal of Science |
| Subjects: | |
| Online Access: | https://ctujs.ctu.edu.vn/index.php/ctujs/article/view/580 |
| Summary: | Ralstonia solanacearum species complex (RSSC) is the main pathogen causing bacterial wilt disease in tomatoes. This study applied the colony polymerase chain reaction (PCR) technique to rapidly screen and select RSSC strains from isolated bacteria of diseased tomatoes. This method directly used bacterial colonies on the Petri plate as templates to amplify with RSSC’s specific and multiplex primers. The results showed that the Vietnamese isolates were identified as R. pseudosolanacearum, phylotype I. Phylogenetic analysis of the 16S-23S rDNA sequencing also confirmed these results. Therefore, this is the first report to recognize R. pseudosolanacearum phylotype I as the cause of bacterial wilt disease in tomatoes from Danang.
|
|---|---|
| ISSN: | 2615-9422 2815-5602 |