Enhanced Antioxidant Activity in Streptococcus thermophilus by High-Level Expression of Superoxide Dismutase
Superoxide dismutase (SOD) plays an essential role in eliminating oxidative damage of lactic acid bacteria. Streptococcus thermophilus, an important probiotic lactic acid bacterium, often inevitably suffers from various oxidative stress during dairy fermentation. In this study, to confer high-level...
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Frontiers Media S.A.
2020-11-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2020.579804/full |
| _version_ | 1818255952858578944 |
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| author | Linghui Kong Zhiqiang Xiong Xin Song Yongjun Xia Hui Zhang Ying Yang Lianzhong Ai |
| author_facet | Linghui Kong Zhiqiang Xiong Xin Song Yongjun Xia Hui Zhang Ying Yang Lianzhong Ai |
| author_sort | Linghui Kong |
| collection | DOAJ |
| description | Superoxide dismutase (SOD) plays an essential role in eliminating oxidative damage of lactic acid bacteria. Streptococcus thermophilus, an important probiotic lactic acid bacterium, often inevitably suffers from various oxidative stress during dairy fermentation. In this study, to confer high-level oxidative resistance, the sod gene from Lactobacillus casei was heterologous expressed in S. thermophilus S-3 using our previous constructed native constitutive promoter library. The enzyme activity of SOD was significantly enhanced in engineered S. thermophilus by promoter #14 (2070 U/mg). Furthermore, the strategy of multi-copy sod-expressing cassettes was employed to improve SOD activity. The maximum activity (2750 U/mg) was obtained by the two-copy sod recombinant, which was 1.5-fold higher than that of one-copy recombinant. In addition, the survival rate of multi-copy sod recombinants was increased about 97-fold with 3.5 mmol/L H2O2 treatment. To our knowledge, this is the first report of multi-copy sod gene expression in S. thermophilus, which exerts a positive effect on coping with oxidative stress to enhance the potential of industrial application. |
| first_indexed | 2024-12-12T17:20:02Z |
| format | Article |
| id | doaj.art-77a38213cce54c37acfcba5eccebeebf |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-12-12T17:20:02Z |
| publishDate | 2020-11-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-77a38213cce54c37acfcba5eccebeebf2022-12-22T00:17:41ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2020-11-011110.3389/fmicb.2020.579804579804Enhanced Antioxidant Activity in Streptococcus thermophilus by High-Level Expression of Superoxide DismutaseLinghui Kong0Zhiqiang Xiong1Xin Song2Yongjun Xia3Hui Zhang4Ying Yang5Lianzhong Ai6Shanghai Engineering Research Center of Food Microbiology, School of Medical Instrument and Food Engineering, University of Shanghai for Science and Technology, Shanghai, ChinaShanghai Engineering Research Center of Food Microbiology, School of Medical Instrument and Food Engineering, University of Shanghai for Science and Technology, Shanghai, ChinaShanghai Engineering Research Center of Food Microbiology, School of Medical Instrument and Food Engineering, University of Shanghai for Science and Technology, Shanghai, ChinaShanghai Engineering Research Center of Food Microbiology, School of Medical Instrument and Food Engineering, University of Shanghai for Science and Technology, Shanghai, ChinaShanghai Engineering Research Center of Food Microbiology, School of Medical Instrument and Food Engineering, University of Shanghai for Science and Technology, Shanghai, ChinaInstitute of Food Science, Zhejiang Academy of Agricultural Sciences, Hangzhou, ChinaShanghai Engineering Research Center of Food Microbiology, School of Medical Instrument and Food Engineering, University of Shanghai for Science and Technology, Shanghai, ChinaSuperoxide dismutase (SOD) plays an essential role in eliminating oxidative damage of lactic acid bacteria. Streptococcus thermophilus, an important probiotic lactic acid bacterium, often inevitably suffers from various oxidative stress during dairy fermentation. In this study, to confer high-level oxidative resistance, the sod gene from Lactobacillus casei was heterologous expressed in S. thermophilus S-3 using our previous constructed native constitutive promoter library. The enzyme activity of SOD was significantly enhanced in engineered S. thermophilus by promoter #14 (2070 U/mg). Furthermore, the strategy of multi-copy sod-expressing cassettes was employed to improve SOD activity. The maximum activity (2750 U/mg) was obtained by the two-copy sod recombinant, which was 1.5-fold higher than that of one-copy recombinant. In addition, the survival rate of multi-copy sod recombinants was increased about 97-fold with 3.5 mmol/L H2O2 treatment. To our knowledge, this is the first report of multi-copy sod gene expression in S. thermophilus, which exerts a positive effect on coping with oxidative stress to enhance the potential of industrial application.https://www.frontiersin.org/articles/10.3389/fmicb.2020.579804/fullStreptococcus thermophilussuperoxide dismutasenative constitutive promotermulticopy gene expressionoxidative stress |
| spellingShingle | Linghui Kong Zhiqiang Xiong Xin Song Yongjun Xia Hui Zhang Ying Yang Lianzhong Ai Enhanced Antioxidant Activity in Streptococcus thermophilus by High-Level Expression of Superoxide Dismutase Frontiers in Microbiology Streptococcus thermophilus superoxide dismutase native constitutive promoter multicopy gene expression oxidative stress |
| title | Enhanced Antioxidant Activity in Streptococcus thermophilus by High-Level Expression of Superoxide Dismutase |
| title_full | Enhanced Antioxidant Activity in Streptococcus thermophilus by High-Level Expression of Superoxide Dismutase |
| title_fullStr | Enhanced Antioxidant Activity in Streptococcus thermophilus by High-Level Expression of Superoxide Dismutase |
| title_full_unstemmed | Enhanced Antioxidant Activity in Streptococcus thermophilus by High-Level Expression of Superoxide Dismutase |
| title_short | Enhanced Antioxidant Activity in Streptococcus thermophilus by High-Level Expression of Superoxide Dismutase |
| title_sort | enhanced antioxidant activity in streptococcus thermophilus by high level expression of superoxide dismutase |
| topic | Streptococcus thermophilus superoxide dismutase native constitutive promoter multicopy gene expression oxidative stress |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2020.579804/full |
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