Diagnosis of Parkinson’s Disease by A Metabolomics-Based Laboratory-Developed Test (LDT)
A laboratory-developed test (LDT) is a type of in vitro diagnostic test that is designed, manufactured and used in the same laboratory (i.e., an in-house test). In this study, a metabolomics-based LDT was developed. This test involves a blood plasma preparation, direct-infusion mass spectrometry ana...
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| Format: | Article |
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MDPI AG
2020-05-01
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| Series: | Diagnostics |
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| Online Access: | https://www.mdpi.com/2075-4418/10/5/332 |
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| author | Petr G. Lokhov Oxana P. Trifonova Dmitry L. Maslov Steven Lichtenberg Elena E. Balashova |
| author_facet | Petr G. Lokhov Oxana P. Trifonova Dmitry L. Maslov Steven Lichtenberg Elena E. Balashova |
| author_sort | Petr G. Lokhov |
| collection | DOAJ |
| description | A laboratory-developed test (LDT) is a type of in vitro diagnostic test that is designed, manufactured and used in the same laboratory (i.e., an in-house test). In this study, a metabolomics-based LDT was developed. This test involves a blood plasma preparation, direct-infusion mass spectrometry analysis with a high-resolution mass spectrometer, alignment and normalization of mass peaks using original algorithms, metabolite annotation by a biochemical context-driven algorithm, detection of overrepresented metabolic pathways and results in a visualization in the form of a pathway names cloud. The LDT was applied to detect early stage Parkinson’s disease (PD)—the diagnosis of which currently requires great effort due to the lack of available laboratory tests. In a case–control study (<i>n</i> = 56), the LDT revealed a statistically sound pattern in the PD-relevant pathways. Usage of the LDT for individuals confirmed its ability to reveal this pattern and thus diagnose PD at the early-stage (1–2.5 stages, according to Hoehn and Yahr scale). The detection of this pattern by LDT could diagnose PD with a specificity of 64%, sensitivity of 86% and an accuracy of 75%. Thus, this LDT can be used for further widespread testing. |
| first_indexed | 2024-03-10T19:41:48Z |
| format | Article |
| id | doaj.art-77a3ff95971344c1aba8e58eda3a6439 |
| institution | Directory Open Access Journal |
| issn | 2075-4418 |
| language | English |
| last_indexed | 2024-03-10T19:41:48Z |
| publishDate | 2020-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Diagnostics |
| spelling | doaj.art-77a3ff95971344c1aba8e58eda3a64392023-11-20T01:13:12ZengMDPI AGDiagnostics2075-44182020-05-0110533210.3390/diagnostics10050332Diagnosis of Parkinson’s Disease by A Metabolomics-Based Laboratory-Developed Test (LDT)Petr G. Lokhov0Oxana P. Trifonova1Dmitry L. Maslov2Steven Lichtenberg3Elena E. Balashova4Institute of Biomedical Chemistry, 10 building 8, Pogodinskaya Street, 119121 Moscow, RussiaInstitute of Biomedical Chemistry, 10 building 8, Pogodinskaya Street, 119121 Moscow, RussiaInstitute of Biomedical Chemistry, 10 building 8, Pogodinskaya Street, 119121 Moscow, RussiaMetabometrics, Inc., 651 N Broad St., Suite 205 #1370, Middletown, DE 19709, USAInstitute of Biomedical Chemistry, 10 building 8, Pogodinskaya Street, 119121 Moscow, RussiaA laboratory-developed test (LDT) is a type of in vitro diagnostic test that is designed, manufactured and used in the same laboratory (i.e., an in-house test). In this study, a metabolomics-based LDT was developed. This test involves a blood plasma preparation, direct-infusion mass spectrometry analysis with a high-resolution mass spectrometer, alignment and normalization of mass peaks using original algorithms, metabolite annotation by a biochemical context-driven algorithm, detection of overrepresented metabolic pathways and results in a visualization in the form of a pathway names cloud. The LDT was applied to detect early stage Parkinson’s disease (PD)—the diagnosis of which currently requires great effort due to the lack of available laboratory tests. In a case–control study (<i>n</i> = 56), the LDT revealed a statistically sound pattern in the PD-relevant pathways. Usage of the LDT for individuals confirmed its ability to reveal this pattern and thus diagnose PD at the early-stage (1–2.5 stages, according to Hoehn and Yahr scale). The detection of this pattern by LDT could diagnose PD with a specificity of 64%, sensitivity of 86% and an accuracy of 75%. Thus, this LDT can be used for further widespread testing.https://www.mdpi.com/2075-4418/10/5/332metabolomicsParkinson’s diseasediagnosticslaboratory-developed testmass spectrometryblood plasma |
| spellingShingle | Petr G. Lokhov Oxana P. Trifonova Dmitry L. Maslov Steven Lichtenberg Elena E. Balashova Diagnosis of Parkinson’s Disease by A Metabolomics-Based Laboratory-Developed Test (LDT) Diagnostics metabolomics Parkinson’s disease diagnostics laboratory-developed test mass spectrometry blood plasma |
| title | Diagnosis of Parkinson’s Disease by A Metabolomics-Based Laboratory-Developed Test (LDT) |
| title_full | Diagnosis of Parkinson’s Disease by A Metabolomics-Based Laboratory-Developed Test (LDT) |
| title_fullStr | Diagnosis of Parkinson’s Disease by A Metabolomics-Based Laboratory-Developed Test (LDT) |
| title_full_unstemmed | Diagnosis of Parkinson’s Disease by A Metabolomics-Based Laboratory-Developed Test (LDT) |
| title_short | Diagnosis of Parkinson’s Disease by A Metabolomics-Based Laboratory-Developed Test (LDT) |
| title_sort | diagnosis of parkinson s disease by a metabolomics based laboratory developed test ldt |
| topic | metabolomics Parkinson’s disease diagnostics laboratory-developed test mass spectrometry blood plasma |
| url | https://www.mdpi.com/2075-4418/10/5/332 |
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