Ex Vitro Rooting and Simultaneous Micrografting of the Walnut Hybrid Rootstock ‘Paradox’ (<i>Juglans hindsi × Juglans regia</i>) cl. ‘Vlach’
In vitro micropropagation is already a current multiplication tool for walnut self-rooted cultivars and rootstocks, but walnut grafting is still performed in the field or in greenhouses, mainly using seedlings as rootstocks. The present work describes a new approach to obtain clonal walnut-grafted p...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2022-02-01
|
| Series: | Agronomy |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2073-4395/12/3/595 |
| _version_ | 1827650242046066688 |
|---|---|
| author | Hugo Ribeiro Augusto Ribeiro Rita Pires João Cruz Hélia Cardoso João Mota Barroso Augusto Peixe |
| author_facet | Hugo Ribeiro Augusto Ribeiro Rita Pires João Cruz Hélia Cardoso João Mota Barroso Augusto Peixe |
| author_sort | Hugo Ribeiro |
| collection | DOAJ |
| description | In vitro micropropagation is already a current multiplication tool for walnut self-rooted cultivars and rootstocks, but walnut grafting is still performed in the field or in greenhouses, mainly using seedlings as rootstocks. The present work describes a new approach to obtain clonal walnut-grafted plants, involving in vitro shoot production of ‘Paradox’ (<i>Juglans hindsi × Juglans regia</i>) cl. ’Vlach’, to be used as rootstock, and <i>J. regia</i> cv. ‘Chandler’, to be used as scion. After completing the in vitro multiplication phase and a seven-day root induction treatment, ‘Vlach’ explants are transferred to ex vitro conditions for root expression while being simultaneously grafted using the in vitro produced ‘Chandler’ scions. The importance of the presence of leaves on both the scion and the rootstock for the success rate of the technique was evaluated. Under optimal conditions, average success rates of 82% for rootstock rooting, 72% for micrografting survival, and 84% for grafted plant acclimatization were achieved. This rooting/grafting combination technique seems able to compete with the traditional techniques of nursery grafting, allowing obtaining high-quality walnut-grafted plants independently of the external weather conditions in a significantly shorter time. |
| first_indexed | 2024-03-09T20:13:38Z |
| format | Article |
| id | doaj.art-77a475ed95954ba89c15b53fc068b0d7 |
| institution | Directory Open Access Journal |
| issn | 2073-4395 |
| language | English |
| last_indexed | 2024-03-09T20:13:38Z |
| publishDate | 2022-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Agronomy |
| spelling | doaj.art-77a475ed95954ba89c15b53fc068b0d72023-11-24T00:07:27ZengMDPI AGAgronomy2073-43952022-02-0112359510.3390/agronomy12030595Ex Vitro Rooting and Simultaneous Micrografting of the Walnut Hybrid Rootstock ‘Paradox’ (<i>Juglans hindsi × Juglans regia</i>) cl. ‘Vlach’Hugo Ribeiro0Augusto Ribeiro1Rita Pires2João Cruz3Hélia Cardoso4João Mota Barroso5Augusto Peixe6DespertaFolia Lda., University of Évora, Pólo da Mitra, Ap. 94, 7006-554 Évora, PortugalDespertaFolia Lda., University of Évora, Pólo da Mitra, Ap. 94, 7006-554 Évora, PortugalMED—Mediterranean Institute for Agriculture, Environment and Development, Institute for Advanced Studies and Research, University of Évora, Pólo da Mitra, Ap. 94, 7006-554 Évora, PortugalDespertaFolia Lda., University of Évora, Pólo da Mitra, Ap. 94, 7006-554 Évora, PortugalMED—Mediterranean Institute for Agriculture, Environment and Development, Institute for Advanced Studies and Research, University of Évora, Pólo da Mitra, Ap. 94, 7006-554 Évora, PortugalMED—Mediterranean Institute for Agriculture, Environment and Development, Departament of Plant Science, School of Science and Technology, University of Évora, Pólo da Mitra, Ap. 94, 7006-554 Évora, PortugalMED—Mediterranean Institute for Agriculture, Environment and Development, Departament of Plant Science, School of Science and Technology, University of Évora, Pólo da Mitra, Ap. 94, 7006-554 Évora, PortugalIn vitro micropropagation is already a current multiplication tool for walnut self-rooted cultivars and rootstocks, but walnut grafting is still performed in the field or in greenhouses, mainly using seedlings as rootstocks. The present work describes a new approach to obtain clonal walnut-grafted plants, involving in vitro shoot production of ‘Paradox’ (<i>Juglans hindsi × Juglans regia</i>) cl. ’Vlach’, to be used as rootstock, and <i>J. regia</i> cv. ‘Chandler’, to be used as scion. After completing the in vitro multiplication phase and a seven-day root induction treatment, ‘Vlach’ explants are transferred to ex vitro conditions for root expression while being simultaneously grafted using the in vitro produced ‘Chandler’ scions. The importance of the presence of leaves on both the scion and the rootstock for the success rate of the technique was evaluated. Under optimal conditions, average success rates of 82% for rootstock rooting, 72% for micrografting survival, and 84% for grafted plant acclimatization were achieved. This rooting/grafting combination technique seems able to compete with the traditional techniques of nursery grafting, allowing obtaining high-quality walnut-grafted plants independently of the external weather conditions in a significantly shorter time.https://www.mdpi.com/2073-4395/12/3/595Chandlergraftingin vitro<i>Juglans</i>micropropagationpropagation |
| spellingShingle | Hugo Ribeiro Augusto Ribeiro Rita Pires João Cruz Hélia Cardoso João Mota Barroso Augusto Peixe Ex Vitro Rooting and Simultaneous Micrografting of the Walnut Hybrid Rootstock ‘Paradox’ (<i>Juglans hindsi × Juglans regia</i>) cl. ‘Vlach’ Agronomy Chandler grafting in vitro <i>Juglans</i> micropropagation propagation |
| title | Ex Vitro Rooting and Simultaneous Micrografting of the Walnut Hybrid Rootstock ‘Paradox’ (<i>Juglans hindsi × Juglans regia</i>) cl. ‘Vlach’ |
| title_full | Ex Vitro Rooting and Simultaneous Micrografting of the Walnut Hybrid Rootstock ‘Paradox’ (<i>Juglans hindsi × Juglans regia</i>) cl. ‘Vlach’ |
| title_fullStr | Ex Vitro Rooting and Simultaneous Micrografting of the Walnut Hybrid Rootstock ‘Paradox’ (<i>Juglans hindsi × Juglans regia</i>) cl. ‘Vlach’ |
| title_full_unstemmed | Ex Vitro Rooting and Simultaneous Micrografting of the Walnut Hybrid Rootstock ‘Paradox’ (<i>Juglans hindsi × Juglans regia</i>) cl. ‘Vlach’ |
| title_short | Ex Vitro Rooting and Simultaneous Micrografting of the Walnut Hybrid Rootstock ‘Paradox’ (<i>Juglans hindsi × Juglans regia</i>) cl. ‘Vlach’ |
| title_sort | ex vitro rooting and simultaneous micrografting of the walnut hybrid rootstock paradox i juglans hindsi juglans regia i cl vlach |
| topic | Chandler grafting in vitro <i>Juglans</i> micropropagation propagation |
| url | https://www.mdpi.com/2073-4395/12/3/595 |
| work_keys_str_mv | AT hugoribeiro exvitrorootingandsimultaneousmicrograftingofthewalnuthybridrootstockparadoxijuglanshindsijuglansregiaiclvlach AT augustoribeiro exvitrorootingandsimultaneousmicrograftingofthewalnuthybridrootstockparadoxijuglanshindsijuglansregiaiclvlach AT ritapires exvitrorootingandsimultaneousmicrograftingofthewalnuthybridrootstockparadoxijuglanshindsijuglansregiaiclvlach AT joaocruz exvitrorootingandsimultaneousmicrograftingofthewalnuthybridrootstockparadoxijuglanshindsijuglansregiaiclvlach AT heliacardoso exvitrorootingandsimultaneousmicrograftingofthewalnuthybridrootstockparadoxijuglanshindsijuglansregiaiclvlach AT joaomotabarroso exvitrorootingandsimultaneousmicrograftingofthewalnuthybridrootstockparadoxijuglanshindsijuglansregiaiclvlach AT augustopeixe exvitrorootingandsimultaneousmicrograftingofthewalnuthybridrootstockparadoxijuglanshindsijuglansregiaiclvlach |