Protective Effects of an Ancient Chinese Kidney-Tonifying Formula against H2O2-Induced Oxidative Damage to MES23.5 Cells
Oxidative damage plays a critical role in the etiology of neurodegenerative disorders including Parkinson’s disease (PD). In our study, an ancient Chinese kidney-tonifying formula, which consists of Cistanche, Epimedii, and Polygonatum cirrhifolium, was investigated to protect MES23.5 dopaminergic n...
| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Hindawi Limited
2017-01-01
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| Series: | Parkinson's Disease |
| Online Access: | http://dx.doi.org/10.1155/2017/2879495 |
| _version_ | 1798044108157091840 |
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| author | Yihui Xu Wei Lin Shuifen Ye Huajin Wang Tingting Wang Youyan Su Liangning Wu Yuanwang Wang Qian Xu Chuanshan Xu Jing Cai |
| author_facet | Yihui Xu Wei Lin Shuifen Ye Huajin Wang Tingting Wang Youyan Su Liangning Wu Yuanwang Wang Qian Xu Chuanshan Xu Jing Cai |
| author_sort | Yihui Xu |
| collection | DOAJ |
| description | Oxidative damage plays a critical role in the etiology of neurodegenerative disorders including Parkinson’s disease (PD). In our study, an ancient Chinese kidney-tonifying formula, which consists of Cistanche, Epimedii, and Polygonatum cirrhifolium, was investigated to protect MES23.5 dopaminergic neurons against hydrogen peroxide- (H2O2-) induced oxidative damage. The damage effects of H2O2 on MES23.5 cells and the protective effects of KTF against oxidative stress were evaluated using MTT assay, transmission electron microscopy (TEM), immunocytochemistry (ICC), enzyme-linked immunosorbent assay (ELISA), and immunoblotting. The results showed that cell viability was dramatically decreased after a 12 h exposure to 150 μM H2O2. TEM observation found that the H2O2-treated MES23.5 cells presented cellular organelle damage. However, when cells were incubated with KTF (3.125, 6.25, and 12.5 μg/ml) for 24 h after H2O2 exposure, a significant protective effect against H2O2-induced damage was observed in MES23.5 cells. Using ICC, we found that KTF inhibited the reduction of the tyrosine hydroxylase (TH) induced by H2O2, upregulated the mRNA and protein expression of HO-1, CAT, and GPx-1, and downregulated the expression of caspase 3. These results indicated that KTF may provide neuron protection against H2O2-induced cell damage through ameliorating oxidative stress, and our findings provide a new potential strategy for the prevention and treatment of Parkinson’s disease. |
| first_indexed | 2024-04-11T22:57:57Z |
| format | Article |
| id | doaj.art-77a62596eba748caaf7b4528bbf74b31 |
| institution | Directory Open Access Journal |
| issn | 2090-8083 2042-0080 |
| language | English |
| last_indexed | 2024-04-11T22:57:57Z |
| publishDate | 2017-01-01 |
| publisher | Hindawi Limited |
| record_format | Article |
| series | Parkinson's Disease |
| spelling | doaj.art-77a62596eba748caaf7b4528bbf74b312022-12-22T03:58:18ZengHindawi LimitedParkinson's Disease2090-80832042-00802017-01-01201710.1155/2017/28794952879495Protective Effects of an Ancient Chinese Kidney-Tonifying Formula against H2O2-Induced Oxidative Damage to MES23.5 CellsYihui Xu0Wei Lin1Shuifen Ye2Huajin Wang3Tingting Wang4Youyan Su5Liangning Wu6Yuanwang Wang7Qian Xu8Chuanshan Xu9Jing Cai10Second People’s Hospital, Fujian University of Traditional Chinese Medicine, Fuzhou, Fujian Province 350003, ChinaAcademy of Integrative Medicine, Fujian University of Traditional Chinese Medicine, Fuzhou, Fujian Province 350122, ChinaLongyan First Hospital Affiliated to Fujian Medical University, Longyan, Fujian Province 364000, ChinaTibet Autonomous Region People’s Hospital, Lhasa, Tibet Autonomous Region 850000, ChinaCollege of Integrative Medicine, Fujian University of Traditional Chinese Medicine, Fuzhou, Fujian Province 350122, ChinaCollege of Integrative Medicine, Fujian University of Traditional Chinese Medicine, Fuzhou, Fujian Province 350122, ChinaGraduate School, Fujian University of Traditional Chinese Medicine, Fuzhou, Fujian Province 350122, ChinaQuanzhou Orthopedic-Traumatological Hospital, Fujian University of Traditional Chinese Medicine, Quanzhou, Fujian Province 362000, ChinaCollege of Integrative Medicine, Fujian University of Traditional Chinese Medicine, Fuzhou, Fujian Province 350122, ChinaSchool of Chinese Medicine, Faculty of Medicine, The Chinese University of Hong Kong, Shatin, Hong KongCollege of Integrative Medicine, Fujian University of Traditional Chinese Medicine, Fuzhou, Fujian Province 350122, ChinaOxidative damage plays a critical role in the etiology of neurodegenerative disorders including Parkinson’s disease (PD). In our study, an ancient Chinese kidney-tonifying formula, which consists of Cistanche, Epimedii, and Polygonatum cirrhifolium, was investigated to protect MES23.5 dopaminergic neurons against hydrogen peroxide- (H2O2-) induced oxidative damage. The damage effects of H2O2 on MES23.5 cells and the protective effects of KTF against oxidative stress were evaluated using MTT assay, transmission electron microscopy (TEM), immunocytochemistry (ICC), enzyme-linked immunosorbent assay (ELISA), and immunoblotting. The results showed that cell viability was dramatically decreased after a 12 h exposure to 150 μM H2O2. TEM observation found that the H2O2-treated MES23.5 cells presented cellular organelle damage. However, when cells were incubated with KTF (3.125, 6.25, and 12.5 μg/ml) for 24 h after H2O2 exposure, a significant protective effect against H2O2-induced damage was observed in MES23.5 cells. Using ICC, we found that KTF inhibited the reduction of the tyrosine hydroxylase (TH) induced by H2O2, upregulated the mRNA and protein expression of HO-1, CAT, and GPx-1, and downregulated the expression of caspase 3. These results indicated that KTF may provide neuron protection against H2O2-induced cell damage through ameliorating oxidative stress, and our findings provide a new potential strategy for the prevention and treatment of Parkinson’s disease.http://dx.doi.org/10.1155/2017/2879495 |
| spellingShingle | Yihui Xu Wei Lin Shuifen Ye Huajin Wang Tingting Wang Youyan Su Liangning Wu Yuanwang Wang Qian Xu Chuanshan Xu Jing Cai Protective Effects of an Ancient Chinese Kidney-Tonifying Formula against H2O2-Induced Oxidative Damage to MES23.5 Cells Parkinson's Disease |
| title | Protective Effects of an Ancient Chinese Kidney-Tonifying Formula against H2O2-Induced Oxidative Damage to MES23.5 Cells |
| title_full | Protective Effects of an Ancient Chinese Kidney-Tonifying Formula against H2O2-Induced Oxidative Damage to MES23.5 Cells |
| title_fullStr | Protective Effects of an Ancient Chinese Kidney-Tonifying Formula against H2O2-Induced Oxidative Damage to MES23.5 Cells |
| title_full_unstemmed | Protective Effects of an Ancient Chinese Kidney-Tonifying Formula against H2O2-Induced Oxidative Damage to MES23.5 Cells |
| title_short | Protective Effects of an Ancient Chinese Kidney-Tonifying Formula against H2O2-Induced Oxidative Damage to MES23.5 Cells |
| title_sort | protective effects of an ancient chinese kidney tonifying formula against h2o2 induced oxidative damage to mes23 5 cells |
| url | http://dx.doi.org/10.1155/2017/2879495 |
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