In vitro Effects of Punica granatum Ellagitannins on Adult Worms of Schistosoma mansoni
Khalid H Abozeid, 1 Mohamed F El-Badawy, 2, 3 Soheir Mahmoud, 4 Mohamed M Shohayeb 5 1Department of Microbiology, College of Medicine, Taif University, Taif, Kingdom of Saudi Arabia; 2Department of Microbiology and Immunology, Faculty of Pharmacy, University of Sadat City, Sadat City, Menoufia Gov...
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| Format: | Article |
| Language: | English |
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Dove Medical Press
2020-10-01
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| Series: | Research and Reports in Tropical Medicine |
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| Online Access: | https://www.dovepress.com/in-vitro-effects-of-punica-granatum-ellagitannins-on-adult-worms-of-sc-peer-reviewed-article-RRTM |
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| author | Abozeid KH El-Badawy MF Mahmoud S Shohayeb MM |
| author_facet | Abozeid KH El-Badawy MF Mahmoud S Shohayeb MM |
| author_sort | Abozeid KH |
| collection | DOAJ |
| description | Khalid H Abozeid, 1 Mohamed F El-Badawy, 2, 3 Soheir Mahmoud, 4 Mohamed M Shohayeb 5 1Department of Microbiology, College of Medicine, Taif University, Taif, Kingdom of Saudi Arabia; 2Department of Microbiology and Immunology, Faculty of Pharmacy, University of Sadat City, Sadat City, Menoufia Governorate, Egypt; 3Division of Pharmaceutical Microbiology, Department of Pharmaceutics and Industrial Pharmacy, College of Pharmacy, Taif University, Taif, Kingdom of Saudi Arabia; 4Parasitology Department, Theodor Bilharz Research Institute, Imbaba, Giza, Egypt; 5Department of Microbiology and Biotechnology, Faculty of Pharmacy, Delta University for Science and Technology, Gamasa, EgyptCorrespondence: Khalid H Abozeid; Mohamed F El-Badawy Email Khaled_abozeid@hotmail.com; m.elbadawy@tu.edu.saAbstract: Schistosomiasis ranks second behind malaria in terms of overall morbidity and mortality. We evaluated the lethal effect of Punica granatum ellagitannins, extracted from the fruit rind, placenta and barks of the root and stem, on adult worms of Schistosoma mansoni (S. mansoni). All four ellagitannins were lethal to S. mansoni adult worms. However, while the rind ellagitannins were the most potent, placental ellagitannins were the least. Rind ellagitannins were capable of killing 40% of adult worms at a concentration of 25μg/mL after 5 days. The killing of 100% of the worms was achievable by rind ellagitannins at a concentration of 50μg/mL after 5 days. The LD 50S of the rind ellagitannins after 96h and 120h were 41.25 μg/mL and 28.73 respectively. Ellagitannins-treated worms suffered from erosions, wrinkles, swellings and losses, degenerations of the surface tubercles and tegument. In addition, ellagitannins induced deformation and degradation of oral and ventral suckers and degenerations in the muscles of worms. Ellagitannins also caused a separation of coupled worms and reduction of their motility. Data obtained suggest that ellagitannins of pomegranate could be considered as a cheap candidate for the treatment of schistosomiasis.Keywords: Punica granatum, ellagitannins, Schistosoma mansoni, tegument, praziquantel |
| first_indexed | 2024-12-21T10:24:55Z |
| format | Article |
| id | doaj.art-77a7deb06af64f63bc17c011c870c6fe |
| institution | Directory Open Access Journal |
| issn | 1179-7282 |
| language | English |
| last_indexed | 2024-12-21T10:24:55Z |
| publishDate | 2020-10-01 |
| publisher | Dove Medical Press |
| record_format | Article |
| series | Research and Reports in Tropical Medicine |
| spelling | doaj.art-77a7deb06af64f63bc17c011c870c6fe2022-12-21T19:07:21ZengDove Medical PressResearch and Reports in Tropical Medicine1179-72822020-10-01Volume 11738057705In vitro Effects of Punica granatum Ellagitannins on Adult Worms of Schistosoma mansoniAbozeid KHEl-Badawy MFMahmoud SShohayeb MMKhalid H Abozeid, 1 Mohamed F El-Badawy, 2, 3 Soheir Mahmoud, 4 Mohamed M Shohayeb 5 1Department of Microbiology, College of Medicine, Taif University, Taif, Kingdom of Saudi Arabia; 2Department of Microbiology and Immunology, Faculty of Pharmacy, University of Sadat City, Sadat City, Menoufia Governorate, Egypt; 3Division of Pharmaceutical Microbiology, Department of Pharmaceutics and Industrial Pharmacy, College of Pharmacy, Taif University, Taif, Kingdom of Saudi Arabia; 4Parasitology Department, Theodor Bilharz Research Institute, Imbaba, Giza, Egypt; 5Department of Microbiology and Biotechnology, Faculty of Pharmacy, Delta University for Science and Technology, Gamasa, EgyptCorrespondence: Khalid H Abozeid; Mohamed F El-Badawy Email Khaled_abozeid@hotmail.com; m.elbadawy@tu.edu.saAbstract: Schistosomiasis ranks second behind malaria in terms of overall morbidity and mortality. We evaluated the lethal effect of Punica granatum ellagitannins, extracted from the fruit rind, placenta and barks of the root and stem, on adult worms of Schistosoma mansoni (S. mansoni). All four ellagitannins were lethal to S. mansoni adult worms. However, while the rind ellagitannins were the most potent, placental ellagitannins were the least. Rind ellagitannins were capable of killing 40% of adult worms at a concentration of 25μg/mL after 5 days. The killing of 100% of the worms was achievable by rind ellagitannins at a concentration of 50μg/mL after 5 days. The LD 50S of the rind ellagitannins after 96h and 120h were 41.25 μg/mL and 28.73 respectively. Ellagitannins-treated worms suffered from erosions, wrinkles, swellings and losses, degenerations of the surface tubercles and tegument. In addition, ellagitannins induced deformation and degradation of oral and ventral suckers and degenerations in the muscles of worms. Ellagitannins also caused a separation of coupled worms and reduction of their motility. Data obtained suggest that ellagitannins of pomegranate could be considered as a cheap candidate for the treatment of schistosomiasis.Keywords: Punica granatum, ellagitannins, Schistosoma mansoni, tegument, praziquantelhttps://www.dovepress.com/in-vitro-effects-of-punica-granatum-ellagitannins-on-adult-worms-of-sc-peer-reviewed-article-RRTMpunica granatumellagitanninsschistosoma mansonitegumentpraziquantel |
| spellingShingle | Abozeid KH El-Badawy MF Mahmoud S Shohayeb MM In vitro Effects of Punica granatum Ellagitannins on Adult Worms of Schistosoma mansoni Research and Reports in Tropical Medicine punica granatum ellagitannins schistosoma mansoni tegument praziquantel |
| title | In vitro Effects of Punica granatum Ellagitannins on Adult Worms of Schistosoma mansoni |
| title_full | In vitro Effects of Punica granatum Ellagitannins on Adult Worms of Schistosoma mansoni |
| title_fullStr | In vitro Effects of Punica granatum Ellagitannins on Adult Worms of Schistosoma mansoni |
| title_full_unstemmed | In vitro Effects of Punica granatum Ellagitannins on Adult Worms of Schistosoma mansoni |
| title_short | In vitro Effects of Punica granatum Ellagitannins on Adult Worms of Schistosoma mansoni |
| title_sort | in vitro effects of punica granatum ellagitannins on adult worms of schistosoma mansoni |
| topic | punica granatum ellagitannins schistosoma mansoni tegument praziquantel |
| url | https://www.dovepress.com/in-vitro-effects-of-punica-granatum-ellagitannins-on-adult-worms-of-sc-peer-reviewed-article-RRTM |
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